Mehmet Tekin

Phytochemicals, antioxidant, and antityrosinase activities of Achillea sivasica Çelik and Akpulat

ABSTRACT The present study is the first report on essential oil (EO) composition, phytochemicals, and biological potential of Achillea sivasica tested against free radicals, oxidative damage, and tyrosinase enzyme. Gas-Chromatography Flame Ionization Detector (GC-FID) and gas chromatography/mass spectrometry (GC/MS) analyses revealed that β-pinene (11.5%, 9.3%, and 6.7%), β-pinene (7.0%, 3.0%, and 6.9%), 1,8-cineole (18.0%, 22.1%, and 6.7%), and camphor (7.6%, 4.1%, and 9.0%) were the major constituents in the EOs from the herb, flower, and leaves, respectively. Liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis of the extracts revealed the presence of caffeoylquinic acid derivatives, luteolin, apigenin, patuletin, isorhamnetin, cirsimaritin, and santin. The leaf extracts demonstrated strongest free radical scavenging, cupric reducing, lipid peroxidation inhibition, and antityrosinase activities.

Evaluation of hepatoprotective and antidiabetic activity of Alchemilla mollis

Alchemilla mollis (Buser) Rothm aerial part and root methanolic-water extracts were evaluated for their hepatoprotective activity on carbon tetrachloride induced hepatotoxicity and hypoglycemic activity on alloxan-induced diabetic mice. None of the tested extracts exhibited effects on blood glucose levels. However hepatoprotective activity results have revealed that serum ALT levels were significantly lowered by both the aerial part and root extracts at doses of 100mg/kg and 200mg/kg. Histopathological examination showed that A. mollis aerial parts and roots induced significant recovery from cellular damage; when compared to the carbon tetrachloride group, the most significant activity was observed with A. mollis aerial part extracts at a dose of 200mg/kg. There is evidence of a hepatoprotective activity of A. mollis on the phenolic content of the plant, especially in the case of flavonoids, which have potent antioxidant properties.

Morphology, anatomy and karyology of endangered Turkish endemic Physoptychis haussknechtii Bornm. (Brassicaceae) from Central Anatolia

Abstract In this study, the morphology, anatomy, pollen morphology, leaf and seed micromorphology and karyology of the Turkish endangered endemic Physoptychis haussknechtii were investigated in detail for the first time. The species was studied in terms of detailed morphology. Basal leaves are narrowly spatulate and cauline leaves are linear to oblanceolate; both leaves are covered with flattened stalked five-armed trichomes. Raceme length was found to be up to 6 cm and 9 cm in the flowering and fruiting stages, respectively. Sepals are linear-oblong, rarely elliptic with obtuse to subacute apex. Petals are spatulate with emarginate apex. Some morphological characteristics such as dimensions of cauline leaves and ovary and length of pistil were measured for the first time. In an anatomical study, root, stem, basal leaf and cauline leaf were examined. The anatomical results showed that the plants have secondary growth in roots, primary growth in stems, amphistomatic and equifacial basal and cauline leaves with anisocytic stomata. Pollen grains are radial symmetrical, isopolar and tricolpate. The ratio of polar axis to equatorial diameter is 1.16, and pollen shape is subprolate. Exine sculpturing is reticulate under light microscope and scanning electron microscope. Seeds are obovatesuborbicular, compressed and pale brown. The chromosome number of P. haussknechtii was defined to be 2n=16. The karyotype formula of P. haussknechtii consists of five metacentric chromosome pairs and three submetacentric chromosome pairs.

Türkiye’den Pimpinella flabellifolia (Boiss.) Benth. et Hook. ex Drude (Apiaceae) türünün karyotipi

Pimpinella flabellifolia (Boiss.) Benth. et Hook. ex Drude Apiaceae familyasina ait bir turdur. Bu calismada, turun kromozom sayisi ve morfolojisi belirlendi. Somatik kromozom sayisi 2n=20 olarak sayildi. Turun karyotipi dokuz metasentrik ve bir submetasentrik kromozom ciftlerinden olusmaktadir. Kromozom boylari 1.72-2.67 µm arasinda degismektedir. Idiyogrami sentromerik indeksi esas alinarak hazirlanmistir ve boyutu azalan sirasina gore duzenlendi.