Melissa Infusino

Interface of Physics and Biology: Engineering Virus-Based Nanoparticles for Biophotonics

Virus-based nanoparticles (VNPs) have been used for a wide range of applications, spanning basic materials science and translational medicine. Their propensity to self-assemble into precise structures that offer a three-dimensional scaffold for functionalization has led to their use as optical contrast agents and related biophotonics applications. A number of fluorescently labeled platforms have been developed and their utility in optical imaging demonstrated, yet their optical properties have not been investigated in detail. In this study, two VNPs of varying architectures were compared side-by-side to determine the impact of dye density, dye localization, conjugation chemistry, and microenvironment on the optical properties of the probes. Dyes were attached to icosahedral cowpea mosaic virus (CPMV) and rod-shaped tobacco mosaic virus (TMV) through a range of chemistries to target particular side chains displayed at specific locations around the virus. The fluorescence intensity and lifetime of the particles were determined, first using photochemical experiments on the benchtop, and second in imaging experiments using tissue culture experiments. The virus-based optical probes were found to be extraordinarily robust under ultrashort, pulsed laser light conditions with a significant amount of excitation energy, maintaining structural and chemical stability. The most effective fluorescence output was achieved through dye placement at optimized densities coupled to the exterior surface avoiding conjugated ring systems. Lifetime measurements indicate that fluorescence output depends not only on spacing the fluorophores, but also on dimer stacking and configurational changes leading to radiationless relaxation—and these processes are related to the conjugation chemistry and nanoparticle shape. For biological applications, the particles were also examined in tissue culture, from which it was found that the optical properties differed from those found on the benchtop due to effects from cellular processes and uptake kinetics. Data indicate that fluorescent cargos are released in the endolysosomal compartment of the cell targeted by the virus-based optical probes. These studies provide insight into the optical properties and fates of fluorescent proteinaceous imaging probes. The cellular release of cargo has implications not only for virus-based optical probes, but also for drug delivery and release systems.

Periodic and aperiodic liquid crystal-polymer composite structures realized via spatial light modulator direct holography

In this work we present the first realization and characterization of two-dimensional periodic and aperiodic POLICRYPS (Polymer Liquid Crystal Polymer Slices) structures, obtained by means of a single-beam holographic technique exploiting a high resolution spatial light modulator (SLM). A first investigation shows that the gratings, operating in the Raman Nath regime, exhibit a morphology and a electro-optical behavior that are typical of the POLICRYPS gratings realized by two-beam interference holography.

Optical and electrical characterization of a gold nanoparticle dispersion in a chiral liquid crystal matrix

We report on the effect of gold nanoparticle (Au NP) dispersion in a chiral nematic liquid crystal (LC). Polarized optical microscopy and X-ray diffraction measurements evidence the insurgence of an order change in the LC host. Moreover, a comparative analysis based on dielectric and voltammetric spectroscopies performed on pure LC and on Au NP-doped LC shows that Au NP’s presence besides affecting LC order influences its electric properties: ion conductivity results importantly reduced, and beyond a threshold value of the applied field electrophoresis phenomena are induced.

Plasmon mediated super-absorber flexible nanocomposites for metamaterials

A flexible host has been selected to achieve, for the first time, functional nanocomposites based on CdSe@ZnS core-shell type quantum dots (QDs) and Au nanoparticles (NPs), simultaneously dispersed in a polymer matrix. Coherent interactions between QDs and plasmonic Au NPs embedded in PDMS films have been demonstrated to lead to a relevant enhancement of the absorption cross-section of the QDs, remarkably modifying the optical response of the entire system. Optical and time resolved spectroscopy studies revealed an active gain-plasmon feedback behind the super-absorbing overall effect.

Effects of Gold Nanoparticle Dispersion in a Chiral Liquid Crystal Matrix

In this article, we report experimental studies on the effect of gold nanoparticle dispersion in a cholesteric liquid crystal matrix. Besides deeply affecting liquid crystal structural order upon inducing phase transition toward unexpected smectic-like phases, the presence of nanoparticles causes important changes in the liquid crystal electric properties, increasing liquid crystal conductivity and influencing ions diffusion.

POLYCRYPS visible curing for spatial light modulator based holography

Realization of highly efficient holographic structures can be obtained by combining the polymer liquid crystal polymer slices (POLICRYPS) technique and the use of a spatial light modulator. To achieve this result, a new prepolymer mixture is necessary that is sensitive to visible light and fulfills all requirements of the POLICRYPS technique. In this paper, we report on our efforts to realize this new mixture and on the first attempts made for fabricating one-dimensional POLICRYPS gratings. Newly obtained diffractive structures have been compared with standard POLICRYPS showing negligible differences.

Plasmon-Exciton Resonant Energy Transfer: Across Scales Hybrid Systems

The presence of an excitonic element in close proximity of a plasmonic nanostructure, under certain conditions, may lead to a nonradiative resonant energy transfer known as Exciton Plasmon Resonant Energy Transfer (EPRET) process. The exciton-plasmon coupling and dynamics have been intensely studied in the last decade; still many relevant aspects need more in-depth studies. Understanding such phenomenon is not only important from fundamental viewpoint, but also essential to unlock many promising applications. In this review we investigate the plasmon-exciton resonant energy transfer in different hybrid systems at the nano- and mesoscales, in order to gain further understanding of such processes across scales and pave the way towards active plasmonic devices.

Liquid Crystals as an Active Medium:Novel Possibilities in Plasmonics

Abstract The peculiar properties of Liquid Crystals (LCs) foster new possibilities in plasmonics. The combination of the intrinsic tunability of LCs with the plasmonic properties of metallic nanoparticles (NPs) provides novel and intriguing features of systems commonly identified as active plasmonics. Being LCs, one of the media whose refractive index can be controlled through the application of external stimuli, they represent a convenient host for enabling plasmonic tunability. On the other hand, the localized plasmonic resonance, typical of NPs, can strongly influence and control the behaviour of LCs. In this paper, we overview several systems of NPs combined with LCs arranged in different configurations. The properties of the resulting systems suggest novel, intriguing outcomes in both fundamental and applied research.

Model for light scattering and lasing in dye-doped nematic liquid crystals

Phenomena associated to lasing in random or quasi-random media are of growing interest both from theoretical and experimental points of view. Recent studies show that nematic liquid crystals are good candidates as scattering host for these lasing systems. We present theoretical analysis and Monte Carlo simulations for different modellizations of scattering in nematics coupled with a two-level system which describes the dye action. We also show that a simple description of light diffusion in nematics is inadequate to describe the localization process that is at the basis of the laser action; therefore, different physical phenomena have to be taken into account.

Optical vortices generated by edge dislocations in electro-convective instability arrays of nematic liquid crystals

We demonstrated by means of interferometry that optical vortices can be generated by diffraction of a laser beam from a birefringent nematic liquid crystal that spontaneously creates a periodic array of electro-convective domains and edge dislocations under an applied electric field. The diffracted beam of order m produced by an elementary dislocation comprises a number |m| of distinct optical vortices, each with unit topological charge. Birefringent liquid crystal arrays provide a fast, convenient, and promising way of generating and studying optical vortices. The used materials are inexpensive, fabrication processes are simple, and both input polarization and applied field can be used as external controls to switch the optical vortices on and off.