Melvyn Rosenblatt

A NEW METHOD OF ACTIVE IMMUNISATION TO AUTOLOGOUS HUMAN TUMOUR TISSUE

Abstract Fourteen volunteers with far-advanced malignant tumours were treated by a method in which an attempt was made to immunise them against their own tumours. The method consisted of chemically coupling the tumour cell to a highly antigenic foreign protein (rabbit gamma-globulin) with bisdiazobenzidine and reinjecting this complex back into the tumour donor. Of the fourteen patients treated, two are alive and have been tumour-free for about 4 years. Biopsy of their tumours revealed necrotic changes. Three patients have shown stabilisation or slow progression of tumour; nine patients have died. Antibodies in high titres directed toward the tumours, were demonstrated by various means in thirteen of these patients.

Production of active immunity to malignant neoplastic tissue. Chemical coupling to an antigenic protein carrier

C3H mice bearing spontaneous mammary adenocarcinomas and methylcholanthrene induced squamous cell carcinomas were immunized actively to their own tumors when their tumor cells were altered, thereby rendering them antigenic. The tumor cells were altered by coupling of human gamma globulin to a tumor cell suspension utilizing bis‐diazobenzidine as a coupling agent. The altered cells then were re‐injected into the animal from which they were taken with complete Freunds adjuvant. An altered host‐tumor relationship was evidenced in the study animals by retardation of tumor growth grossly and increased amounts of necrosis, lymphocytic infiltration, hydropic degeneration and fibrosis on histologic section. Antibodies to tumor cells were detected in the study group following immunization by microprecipitin, Ouchterlony and immunofluorescent techniques. Antibodies were not detectable in sera of control animals immunized with unaltered tumor cells alone in complete Freunds adjuvant.

The Selective Irradiation Of Canine Lymph Nodes By Means Of Intralymphatic Injection Of 32p

Intralymphatic injection of radioactive chromic phosphate produced a severe destruction of a majority of lymph nodes, and subsequent marked lymphopenia in dogs. Selectivity of radiation was evidenced by histological study of other major organs and blood counts. Antibody production of these animals against human serum albumin was significantly inhibited; however, the reactivity to the allografted heart was not altered.

Einfluß einer durch Autoimmunreaktion hervorgerufenen Lymphopenie auf die Transplantatabstoßung beim Hund

ZusammenfassungEine auf einer Autoimmunreaktion beruhende Methode zur Erzielung einer experimentellen Lymphopenie wird geschildert. Dabei wurde keine Granulopenie beobachtet. Nach Auftreten der deutlichen Lymphopenie wurden an Herzen bei Hunden heterotop transplantiert. Die Überlebenszeit der Transplantate war nicht signifikant verlängert.SummaryThis report describes an autoimmune method for inducing experimental lymphopenia in dogs. The lymphopenia was not accompanied by granulocytopenia. Following the appearance of lymphopenia hearts were transplanted heterotopically. The survival time of the transplants was not significantly prolonged.

Serum Electrophoretic Changes Following Heart Allotransplantation.

Summary Serum electrophoretic changes in animals receiving allotransplantation of the heart were studied. Two major changes were observed: an early elevation in alpha-2 globulin and a late (post rejection) increase in gamma globulin fraction. The rise of alpha-2 globulins could not be correlated with cytotoxic changes leading to rejection. It is therefore felt that the initial rise in alpha-2 globulin represented a non-cytotoxic graft specific antibody. The change in gamma globulin seemed to be closely related to rejection and possibly represented cytotoxic graft specific antibody.

Production of Enhanced Immunity to Autologous Material by Means of Coupling to an Antigenic Protein Carrier.

Excerpt The possibility was explored of establishing a state of active immunity by means of coupling components of autologous body material to an antigenic protein carrier and injecting this comple...