Mi Jin Choi

Malvidin Protects WI-38 Human Fibroblast Cells Against Stress-induced Premature Senescence.

Background: Malvidin is one of the most abundant components in red wines and black rice. The effects of malvidin on aging and lifespan under oxidative stress have not been fully understood. This study focused on the anti-aging effect of malvidin on stress-induced premature senescence (SIPS) in WI-38 human lung-derived diploid fibroblasts. Methods: In order to determine the viability of WI-38 cells, MTT assay was conducted, and malondialdehyde level was determined using thiobarbituric acid-reactive substance assay. Protein expression of inflammation-related factors was also evaluated by Western blot analysis. Results: Acute and chronic oxidative stress via hydrogen peroxide (H2O2) treatment led to SIPS in WI-38 cells, which showed decreased cell viability, increased lipid peroxidation, and a shortened lifespan in comparison with non-H2O2-treated WI-38 cells. However, malvidin treatment significantly attenuated H2O2-induced oxidative stress by inhibiting lipid peroxidation and increasing cell viability. Furthermore, the lifespan of WI-38 cells was prolonged by malvidin treatment. In addition, malvidin downregulated the expression of oxidative stress-related proteins, including NF-κB, COX-2, and inducible nitric oxide synthase. Furthermore, protein expression levels of p53, p21, and Bax were also regulated by malvidin treatment in WI-38 cells undergoing SIPS. Conclusions: Malvidin may potentially inhibit the aging process by controlling oxidative stress.

Antioxidant activity of the methanolic extract of the newly generated vegetable, baemuchae (xBrassicoraphanus).

We investigated the radical scavenging activity and protective effects of the methanol extract (ME) and ferulic acid (FA) from baemuchae (xBrassicoraphanus) against oxidative stress in vitro and on a cellular system using LLC-PK1 renal epithelial cells. In vitro, ME and FA showed dose-dependent radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals. LLC-PK1 cells displayed decreased viability in response to nitric oxide-induced oxidative stress. However, ME and FA significantly increased LLC-PK1 cell viability in a dose-dependent manner. Based on HPLC/UV analysis, the FA content was higher in the shoot of baemuchae than in the root. The results of the present study suggest that baemuchae may be a promising source of antioxidants against oxidative stress induced by free radicals.