Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Michal Grulich is active.

Publication


Featured researches published by Michal Grulich.


Biotechnology Advances | 2013

Perspectives and industrial potential of PGA selectivity and promiscuity

Michal Grulich; Václav Štěpánek

Penicillin G acylases (PGAs) are robust industrial catalysts used for biotransformation of β-lactams into key intermediates for chemical production of semi-synthetic β-lactam antibiotics by hydrolysis of natural penicillins. They are used also in reverse, kinetically controlled synthetic reactions for large-scale productions of these antibiotics from corresponding beta-lactam nuclei and activated acyl donors. Further biocatalytic applications of PGAs have recently been described: catalysis of peptide syntheses and the resolutions of racemic mixtures for the production of enantiopure active pharmaceutical ingredients that are based on enantioselective acylation or chiral hydrolysis. Moreover, PGAs rank among promiscuous enzymes because they also catalyze reactions such as trans-esterification, Markovnikov addition or Henry reaction. This particular biocatalytic versatility represents a driving force for the discovery of novel members of this enzyme family and further research into the catalytic potential of PGAs. This review deals with biocatalytic applications exploiting enantioselectivity and promiscuity of prokaryotic PGAs that have been recently reported. Biocatalytic applications are discussed and presented with reaction substrates converted into active compounds useful for the pharmaceutical industry.


PLOS ONE | 2018

The crystal structure of XdpB, the bacterial old yellow enzyme, in an FMN-free form

Jiří Zahradník; Petr Kolenko; Andrea Palyzová; Jiří Černý; Lucie Kolářová; Eva Kyslíková; Helena Marešová; Michal Grulich; Jaroslav Nunvar; Miroslav Šulc; Bohdan Schneider

Old Yellow Enzymes (OYEs) are NAD(P)H dehydrogenases of not fully resolved physiological roles that are widespread among bacteria, plants, and fungi and have a great potential for biotechnological applications. We determined the apo form crystal structure of a member of the OYE class, glycerol trinitrate reductase XdpB, from Agrobacterium bohemicum R89-1 at 2.1 Å resolution. In agreement with the structures of the related bacterial OYEs, the structure revealed the TIM barrel fold with an N-terminal β-hairpin lid, but surprisingly, the structure did not contain its cofactor FMN. Its putative binding site was occupied by a pentapeptide TTSDN from the C-terminus of a symmetry related molecule. Biochemical experiments confirmed a specific concentration-dependent oligomerization and a low FMN content. The blocking of the FMN binding site can exist in vivo and regulates enzyme activity. Our bioinformatic analysis indicated that a similar self-inhibition could be expected in more OYEs which we designated as subgroup OYE C1. This subgroup is widespread among G-bacteria and can be recognized by the conserved sequence GxxDYP in proximity of the C termini. In proteobacteria, the C1 subgroup OYEs are typically coded in one operon with short-chain dehydrogenase. This operon is controlled by the tetR-like transcriptional regulator. OYEs coded in these operons are unlikely to be involved in the oxidative stress response as the other known members of the OYE family because no upregulation of XdpB was observed after exposing A. bohemicum R89-1 to oxidative stress.


Journal of Molecular Catalysis B-enzymatic | 2010

Access to enantiopure aromatic epoxides and diols using epoxide hydrolases derived from total biofilter DNA

Michael Kotik; Václav Štěpánek; Michal Grulich; Alain Archelas


Applied Microbiology and Biotechnology | 2014

Current state and perspectives of penicillin G acylase-based biocatalyses

Helena Marešová; Martina Plačková; Michal Grulich


Applied Microbiology and Biotechnology | 2014

Penicillin G acylase from Achromobacter sp. CCM 4824

Stanislav Bečka; Václav Štěpánek; Rajasekar W. Vyasarayani; Michal Grulich; Jaroslav Maršálek; Kamila Plháčková; Marie Dobišová; Helena Marešová; Martina Plačková; Renáta Valešová; Andrea Palyzová; Anupama Datla; Trupti K. Ashar


Journal of Molecular Catalysis B-enzymatic | 2015

Resolution of α/β-amino acids by enantioselective penicillin G acylase from Achromobacter sp.

Michal Grulich; Jan Brezovský; Václav ŠtĿpánek; Andrea Palyzová; Eva Kyslíková


Process Biochemistry | 2011

Production, enrichment and immobilization of a metagenome-derived epoxide hydrolase

Michal Grulich; Jaroslav Maršálek; Václav Štěpánek; Michael Kotik


Folia Microbiologica | 2017

Potential of the strain Raoultella sp. KDF8 for removal of analgesics

Andrea Palyzová; Jiří Zahradník; Helena Marešová; Lucie Sokolova; Eva Kyslíková; Michal Grulich; Václav Štěpánek; Tomáš Řezanka


Applied Microbiology and Biotechnology | 2014

Erratum to: Penicillin G acylase from Achromobacter sp. CCM 4824

Stanislav Bečka; Václav Štěpánek; Rajasekar W. Vyasarayani; Michal Grulich; Jaroslav Maršálek; Kamila Plháčková; Marie Dobišová; Helena Marešová; Martina Plačková; Renáta Valešová; Andrea Palyzová; Anupama Datla; Trupti K. Ashar


Folia Microbiologica | 2017

Potential of Pichia pastoris for the production of industrial penicillin G acylase

Helena Marešová; Andrea Palyzová; Martina Plačková; Michal Grulich; Vyasa Williams Rajasekar; Václav Štěpánek; Eva Kyslíková

Collaboration


Dive into the Michal Grulich's collaboration.

Top Co-Authors

Avatar

Václav Štěpánek

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Andrea Palyzová

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Helena Marešová

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Eva Kyslíková

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Martina Plačková

Charles University in Prague

View shared research outputs
Top Co-Authors

Avatar

Jaroslav Maršálek

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Jiří Zahradník

Charles University in Prague

View shared research outputs
Top Co-Authors

Avatar

Kamila Plháčková

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Marie Dobišová

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Michael Kotik

Academy of Sciences of the Czech Republic

View shared research outputs
Researchain Logo
Decentralizing Knowledge