Michal Grulich
Academy of Sciences of the Czech Republic
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Featured researches published by Michal Grulich.
Biotechnology Advances | 2013
Michal Grulich; Václav Štěpánek
Penicillin G acylases (PGAs) are robust industrial catalysts used for biotransformation of β-lactams into key intermediates for chemical production of semi-synthetic β-lactam antibiotics by hydrolysis of natural penicillins. They are used also in reverse, kinetically controlled synthetic reactions for large-scale productions of these antibiotics from corresponding beta-lactam nuclei and activated acyl donors. Further biocatalytic applications of PGAs have recently been described: catalysis of peptide syntheses and the resolutions of racemic mixtures for the production of enantiopure active pharmaceutical ingredients that are based on enantioselective acylation or chiral hydrolysis. Moreover, PGAs rank among promiscuous enzymes because they also catalyze reactions such as trans-esterification, Markovnikov addition or Henry reaction. This particular biocatalytic versatility represents a driving force for the discovery of novel members of this enzyme family and further research into the catalytic potential of PGAs. This review deals with biocatalytic applications exploiting enantioselectivity and promiscuity of prokaryotic PGAs that have been recently reported. Biocatalytic applications are discussed and presented with reaction substrates converted into active compounds useful for the pharmaceutical industry.
PLOS ONE | 2018
Jiří Zahradník; Petr Kolenko; Andrea Palyzová; Jiří Černý; Lucie Kolářová; Eva Kyslíková; Helena Marešová; Michal Grulich; Jaroslav Nunvar; Miroslav Šulc; Bohdan Schneider
Old Yellow Enzymes (OYEs) are NAD(P)H dehydrogenases of not fully resolved physiological roles that are widespread among bacteria, plants, and fungi and have a great potential for biotechnological applications. We determined the apo form crystal structure of a member of the OYE class, glycerol trinitrate reductase XdpB, from Agrobacterium bohemicum R89-1 at 2.1 Å resolution. In agreement with the structures of the related bacterial OYEs, the structure revealed the TIM barrel fold with an N-terminal β-hairpin lid, but surprisingly, the structure did not contain its cofactor FMN. Its putative binding site was occupied by a pentapeptide TTSDN from the C-terminus of a symmetry related molecule. Biochemical experiments confirmed a specific concentration-dependent oligomerization and a low FMN content. The blocking of the FMN binding site can exist in vivo and regulates enzyme activity. Our bioinformatic analysis indicated that a similar self-inhibition could be expected in more OYEs which we designated as subgroup OYE C1. This subgroup is widespread among G-bacteria and can be recognized by the conserved sequence GxxDYP in proximity of the C termini. In proteobacteria, the C1 subgroup OYEs are typically coded in one operon with short-chain dehydrogenase. This operon is controlled by the tetR-like transcriptional regulator. OYEs coded in these operons are unlikely to be involved in the oxidative stress response as the other known members of the OYE family because no upregulation of XdpB was observed after exposing A. bohemicum R89-1 to oxidative stress.
Journal of Molecular Catalysis B-enzymatic | 2010
Michael Kotik; Václav Štěpánek; Michal Grulich; Alain Archelas
Applied Microbiology and Biotechnology | 2014
Helena Marešová; Martina Plačková; Michal Grulich
Applied Microbiology and Biotechnology | 2014
Stanislav Bečka; Václav Štěpánek; Rajasekar W. Vyasarayani; Michal Grulich; Jaroslav Maršálek; Kamila Plháčková; Marie Dobišová; Helena Marešová; Martina Plačková; Renáta Valešová; Andrea Palyzová; Anupama Datla; Trupti K. Ashar
Journal of Molecular Catalysis B-enzymatic | 2015
Michal Grulich; Jan Brezovský; Václav ŠtĿpánek; Andrea Palyzová; Eva Kyslíková
Process Biochemistry | 2011
Michal Grulich; Jaroslav Maršálek; Václav Štěpánek; Michael Kotik
Folia Microbiologica | 2017
Andrea Palyzová; Jiří Zahradník; Helena Marešová; Lucie Sokolova; Eva Kyslíková; Michal Grulich; Václav Štěpánek; Tomáš Řezanka
Applied Microbiology and Biotechnology | 2014
Stanislav Bečka; Václav Štěpánek; Rajasekar W. Vyasarayani; Michal Grulich; Jaroslav Maršálek; Kamila Plháčková; Marie Dobišová; Helena Marešová; Martina Plačková; Renáta Valešová; Andrea Palyzová; Anupama Datla; Trupti K. Ashar
Folia Microbiologica | 2017
Helena Marešová; Andrea Palyzová; Martina Plačková; Michal Grulich; Vyasa Williams Rajasekar; Václav Štěpánek; Eva Kyslíková