Michel Lhermitte
Lille University of Science and Technology
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Featured researches published by Michel Lhermitte.
Brain Research | 2008
Mihaela Enache; Vincent Van Waes; Elisabeth Vinner; Michel Lhermitte; Stefania Maccari; Muriel Darnaudéry
Prenatal restraint stress (PRS) in rats is associated with hippocampal dysfunctions and several behavioural and endocrine disorders related to this brain area. Recently, we have reported that the PRS modifies the hypothalamic-pituitary-adrenal (HPA) response to an ethanol challenge in adolescent animals. Since hippocampus is particularly sensitive to the deleterious effects of ethanol during adolescence, we investigated in this study the combined effects of PRS and ethanol administration on the oxidative status in the hippocampus of 28-day-old male rats. Thirty minutes after an intraperitoneal (i.p.) injection of ethanol (1.5 g/kg), the activities of several antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) but also non-enzymatic antioxidant (reduced glutathione) were assayed. Thiobarbituric acid reactive substances (TBARS) levels were also measured as a marker of lipid peroxidation. Ethanol enhanced superoxide dismutase activity in control rats but not in PRS rats. At basal level, catalase activity was lower in PRS rats than in control rats, indicating a potentially higher sensitivity to oxidative damages after this early stress. However, the hippocampal TBARS levels were not significantly affected by the ethanol administration, showing that an acute ethanol exposure does not induce oxidative damage in adolescent male rats. In conclusion, our data suggest that PRS affects both basal antioxidant status in the hippocampus and antioxidant response after an acute ethanol exposure. These findings extend previous works showing that PRS leads to hippocampal dysfunctions and raise the question of the potential increase of the hippocampal oxidative damage in PRS rats after repeated exposure to ethanol.
Forensic Science International | 2003
Michel Imbenotte; Nathalie Azaroual; B. Cartigny; Gaston Vermeersch; Michel Lhermitte
In order to analyse a wide range of xenobiotics and their metabolites present in biological fluids, NMR spectroscopy can be used. A large variety of xenobiotics (therapeutic agents, pesticides, solvents, alcohols) can be characterized and quantitated directly, without sample preparation. NMR investigations were applied to acute poisoning cases, involving drugs such as salicylates and valproic acid (VPA). In a salicylate poisoning case, the three major metabolites of acetylsalicylic acid have been detected in crude urine, and rapid identification of lysine revealed the origin of the intoxication, namely lysine acetylsalicylate (Aspegic). Valproic acid as its glucuronide was identified in urine samples from two poisoned patients. 1H NMR was also used to identify and quantitate paraquat (Gramoxone) in urine owing to its two aromatic signals at 8.49 and 9.02 ppm, in two acutely poisoned patients (183 and 93 mg/l). An intentional poisoning case with tetrahydrofuran (THF) was also investigated. Serum and urine samples were collected. THF was characterized by its resonances at 1.90 and 3.76 ppm, and quantified at 813 and 850 mg/l in the two biological fluids, respectively. Moreover, two other compounds were detected: lactate and gamma-hydroxybutyric acid (GHB). 1H NMR spectroscopic analysis of serum samples from three poisoned patients revealed methanol in one case and ethylene glycol in the two others. Moreover, in the same spectrum, the corresponding metabolites formate and glycolate were found. Compared with the reference chromatographic or spectrophotometric methods, requiring time-consuming extraction and/or derivatization steps, NMR spectroscopy allows the determination of many exogenous and endogenous compounds, without any pre-selection of the analytes.
NMR in Biomedicine | 1997
Susanna Maschke; Nathalie Azaroual; Jean-Michel Wieruszeski; Guy Lippens; Michel Imbenotte; Daniel Mathieu; Gaston Vermeersch; Michel Lhermitte
Analysis of biological fluids by proton nuclear magnetic resonance spectroscopy (1H NMR) is a promising tool in clinical biology. We have used this method for a rapid toxicological screening in the case of a suicide attempt. A urine sample was analysed at 300 and 600 MHz by 1D and 2D sequences (J‐resolved and TOCSY) in a short experimental time. Quantification was realized by peak integration of the 1D spectrum. The results showed the presence of chloroquine and its major metabolite monodesethylchloroquine at concentrations of 462 and 140 mg/L, respectively. Ethanol was also detected in the spectrum. It can be concluded that 1H NMR provides many advantages as a tool for clinical diagnosis in a case of acute intoxication.
Forensic Science International | 2004
B. Cartigny; Nathalie Azaroual; Michel Imbenotte; D Mathieu; Gaston Vermeersch; J.P Goullé; Michel Lhermitte
Forensic Science International | 2004
Laurence Labat; V Dumestre-Toulet; Jean-Pierre Goullé; Michel Lhermitte
Talanta | 2008
Bernard Cartigny; Nathalie Azaroual; Michel Imbenotte; Daniel Mathieu; Erika Parmentier; Gaston Vermeersch; Michel Lhermitte
Chemical & Pharmaceutical Bulletin | 2006
Frédérique Klupsch; Raymond Houssin; Luc Humbert; Michel Imbenotte; Jean-Pierre Hénichart; Michel Lhermitte
NMR in Biomedicine | 1995
Susanna Maschke; Michel Lhermitte; Nathalie Azaroual; Gaston Vermeersch; Michel Imbenotte; F. Leclerc
Annales De Toxicologie Analytique | 2005
Jean-Pierre Anger; Laurence Labat; Michel Lhermitte
Toxicologie Analytique et Clinique | 2015
Hassina Khaldoun-Oularbi; D. Allorge; Camille Richeval; Michel Lhermitte; Nadia Djenas