Michèle LaVigne

Evolutionary change during experimental ocean acidification

Rising atmospheric carbon dioxide (CO2) conditions are driving unprecedented changes in seawater chemistry, resulting in reduced pH and carbonate ion concentrations in the Earth’s oceans. This ocean acidification has negative but variable impacts on individual performance in many marine species. However, little is known about the adaptive capacity of species to respond to an acidified ocean, and, as a result, predictions regarding future ecosystem responses remain incomplete. Here we demonstrate that ocean acidification generates striking patterns of genome-wide selection in purple sea urchins (Strongylocentrotus purpuratus) cultured under different CO2 levels. We examined genetic change at 19,493 loci in larvae from seven adult populations cultured under realistic future CO2 levels. Although larval development and morphology showed little response to elevated CO2, we found substantial allelic change in 40 functional classes of proteins involving hundreds of loci. Pronounced genetic changes, including excess amino acid replacements, were detected in all populations and occurred in genes for biomineralization, lipid metabolism, and ion homeostasis—gene classes that build skeletons and interact in pH regulation. Such genetic change represents a neglected and important impact of ocean acidification that may influence populations that show few outward signs of response to acidification. Our results demonstrate the capacity for rapid evolution in the face of ocean acidification and show that standing genetic variation could be a reservoir of resilience to climate change in this coastal upwelling ecosystem. However, effective response to strong natural selection demands large population sizes and may be limited in species impacted by other environmental stressors.

Skeletal P/Ca tracks upwelling in Gulf of Panamá coral: Evidence for a new seawater phosphate proxy

The supply of limiting nutrients to the low latitude ocean is controlled by physical processes linked to climate variations, but methods for reconstructing past nutrient concentrations in the surface ocean are few and indirect. Here, we present laser ablation mass spectrometry results that reveal annual cycles of P/Ca in a 4-year record from the scleractinian coral Pavona gigantea (mean P/Ca = 118 mmol mol?1). The P/Ca cycles track variations in past seawater phosphate concentration synchronously with skeletal Sr/Ca-derived temperature variations associated with seasonal upwelling in the Gulf of Panama´. Skeletal P/Ca varies seasonally by 2–3 fold, reflecting the timing and magnitude of dissolved phosphate variations. Solution cleaning experiments on drilled coral powders show that over 60% of skeletal P occurs in intracrystalline organic phases. Coral skeleton P/Ca holds promise as a proxy record of nutrient availability on time scales of decades to millennia.

Temperature and vital effect controls on bamboo coral (Isididae) isotope geochemistry: A test of the “lines method”

Deep-sea bamboo corals hold promise as long-term climatic archives, yet little information exists linking bamboo coral geochemistry to measured environmental parameters. This study focuses on a suite of 10 bamboo corals collected from the Pacific and Atlantic basins (250–2136 m water depth) to investigate coral longevity, growth rates, and isotopic signatures. Calcite samples for stable isotopes and radiocarbon were collected from the base the corals, where the entire history of growth is recorded. In three of the coral specimens, samples were also taken from an upper branch for comparison. Radiocarbon and growth band width analyses indicate that the skeletal calcite precipitates from ambient dissolved inorganic carbon and that the corals live for 150–300 years, with extension rates of 9–128 μm/yr. A linear relationship between coral calcite δ18O and δ13C indicates that the isotopic composition is influenced by vital effects (δ18O:δ13C slope of 0.17–0.47). As with scleractinian deep-sea corals, the intercept from a linear regression of δ18O versus δ13C is a function of temperature, such that a reliable paleotemperature proxy can be obtained, using the “lines method.” Although the coral calcite δ18O:δ13C slope is maintained throughout the coral base ontogeny, the branches and central cores of the bases exhibit δ18O:δ13C values that are shifted far from equilibrium. We find that a reliable intercept value can be derived from the δ18O:δ13C regression of multiple samples distributed throughout one specimen or from multiple samples within individual growth bands.