Miki Shiramizu

Multilocular Cystic Nephroma in An Adult: Immunohistochemical Study

We report a case of multilocular cystic nephroma (cysts of the kidney). The tumor was enucleated surgically. It was well encapsulated, and composed of numerous cysts and cellular stroma. Microscopically, the stromal cells resembled blastematous cells seen in Wilms tumor. Immunohistochemical studies disclosed that the stromal cells resembled leiomyocytes, since vimentin and desmin were positive, and myoglobin was negative. An ultrastructural study supported these findings. The characteristics of these cells were compatible with those of congenital mesoblastic nephroma, which also is considered a metanephric tumor.

Autoradiographic and cytochemical localization of androgen in human prostatic cancer cell lines.

For basic studies of receptor dynamics in androgen-responsive tissues and cells, the autoradiographic and cytochemical procedures were applied to cultured tumor cells (DU-145 and PC-3). Uptake and retention of 3H-R1881, a potent synthetic androgen, were observed in DU-145 cells. The radioactive labelling was intense, and solely confined to the nuclei of DU-145 cells. Radioactivity over PC-3 cells was minimal. For assessing binding specificity, DU-145 cells were incubated with 3H-R1881 in the presence or absence of either unlabelled R1881, testosterone, progesterone, estradiol-17 beta, or corticosterone. The displacement of 3H-R1881 with R1881 and testosterone was significant, while no displacement was observed with other steroids. Nuclear localization of cytochemical staining of the dihydrotestosterone-peroxidase conjugate was evident in DU-145 cells. Our results indicate that androgen receptor may reside primarily in target cell nuclei of androgen-responsive tissues and tumors.

Squamous cell carcinoma of ureter extending into renal vein Positive immunohistochemical staining for carcinoembryonic Antigen

A case of squamous cell carcinoma of the ureter with extension into the renal vein is presented. Immunoperoxidase method revealed positive staining for carcinoembryonic antigen. The literature on ureteral tumors is reviewed with special reference to renal vein involvement and carcinoembryonic antigen.