Mineo Yoshino

Pralidoxime Iodide (2-PAM) Penetrates Across the Blood-Brain Barrier

The in vivo rat brain microdialysis technique with HPLC/UV was used to determine the blood-brain barrier (BBB) penetration of pralidoxime iodide (2-PAM), which is a component of the current nerve agent antidote therapy. After intravenous dosage of 2-PAM (10, 50, 100 mg/kg), 2-PAM appeared dose-dependently in the dialysate; the striatal extracellular/blood concentration ratio at 1 h after 50 mg/kg dosage was 0.093 ± 0.053 (mean ± SEM). This finding offered conclusive evidence of the BBB penetration of 2-PAM. We also examined whether the BBB penetration of 2-PAM was mediated by a certain specific transporter, such as a neutral or basic amino acid transport system. Although it was unclear, the neural uptake of 2-PAM was Na+ dependent. The mean BBB penetration by 2-PAM was approximately 10%, indicating the intravenous administration of 2-PAM might be to a degree effective to reactivation of the blocked cholinesterase in the brain.

Sexual dimorphism in the humerus : A comparative analysis of Chinese, Japanese and Thais

Determination of sex from the skeleton is vital to medicolegal investigations. There is no longer any question that populations differ in size and proportions and these differences affect the metric assessment of sex. The extent of variation in sexual dimorphism among Asian Mongoloids within and between regions has not been quantified by discriminant function analysis, nor have standards for most groups been introduced for the humerus. Therefore, the purpose of this research is to establish metric standards for sex determination from the humerus of Chinese, Japanese and Thais, as well as to compare size and sexual dimorphism in these Asian Mongoloid populations. The database for this study consisted of documented skeletal samples from China (N = 87), Japan (N = 90), and Thailand (N = 104). Six standard dimensions, including maximum length, vertical head diameter, minimum midshaft diameter, maximum midshaft diameter, midshaft circumference, and epicondylar breadth were taken and subjected to stepwise and direct discriminant function analysis. Of dimensions selected by the stepwise function, vertical head diameter and epicondylar breadth were the only elements common to all three groups. Overall, mean accuracies were highest using formulae produced by the stepwise procedure and ranged from 86.8% in the Chinese to 92.4% in the Japanese to 97.1% in the Thais. Group comparisons also revealed that while the Chinese had the largest measurements, they were the least dimorphic. The reverse was true for the Thais and the Japanese were intermediate on both counts. In cross validation tests, classification accuracy decreased in all cases where a formula from one group was applied to another. It was therefore concluded even though all individuals were Asian Mongoloids, these regionally diverse populations exhibited significant metric differences that affect sex determination from the skeleton. These findings confirm those of previous studies that there is a need for group specific metric standards of assessment.

Classification system of frontal sinus patterns by radiography. Its application to identification of unknown skeletal remains

Frontal sinuses of Japanese skulls were radiographically examined from the view point of identifying individuals. The system of classification of sinus was proposed, basing on the area size, the bilateral asymmetry, the superiority of side, the outline of upper borders, the partial septa and the supraorbital cells. Frontal sinus patterns could be divided into above 20,000 possible combinations by combining the class numbers in each classification item described above. The frontal sinus pattern of a given person was formulated as a code number which was determined by arranging the class numbers in each classification item as serial numbers. This identification system by the frontal sinus pattern should allow one to characterize each person. The application of this identification system to an actual criminal case was also described.

Sex Determination from the Tibia: Standards for Contemporary Japan

It is vital that skeletal biologists and forensic anthropologists observe populations over time so that changes can be detected and monitored. The purpose of this study is to determine if temporal changes are manifest in the skeleton and, if so, develop appropriate standards to determine sex from the tibia in the contemporary inhabitants of Japan. Osteometric data were obtained from 84 recent Japanese skeletons located at Jikei Medical University, Tokyo. The collection was assembled from the anatomy dissecting room between 1960-1970. With a mean age of about 56 years for males and 51 for females, this sample represents individuals who lived through WWII. Seven tibial measurements were taken and subjected to SPSS-X discriminant function analysis. Results indicated that proximal and distal breadth measurements were selected by the stepwise procedure as the most discriminating. In addition, a number of combinations of measurements were used to develop formulae that would be suitable for fragmentary bones. Average prediction accuracy ranged from 80% from minimum shaft circumference to 89% with proximal epiphyseal breadth. Classification accuracy was higher in males (96%) than in females (79%). Compared with earlier studies of the Japanese, Jikei tibiae are longer, especially in females and thus exhibit less sexual dimorphism. The present research provides new standards that better reflect the Japanese people of today. Furthermore, unlike earlier length based formulae, these results allow sexing from fragmentary bones.

A new database of mitochondrial DNA hypervariable regions I and II sequences from 162 Japanese individuals

A database of mitochondrial DNA (mtDNA) hypervariable region 1 (HV1) and region 2 (HV2) sequences of the mtDNA control region was established from 162 unrelated Japanese individuals. The random match probability and the genetic diversity for this database were 0.96% and 0.997, respectively. Length heteroplasmy in the C-stretch regions located around position 16189 in HV1 and 310 in HV2 was observed in 37% and 38% of the samples, respectively. A strategy using internal sequencing primers was devised to obtain confirmed sequences in these length heteroplasmic individuals. This database, combined with other mtDNA sequence databases from the Japanese population, will permit the significance of mtDNA match results to be properly reported in mtDNA typing casework in Japan.

Microscopical study on estimation of time since death in skeletal remains

For the purpose of estimating time since death in skeletal remains, postmortem changes in human compact bones were examined by microradiography and electron microscopy. The UV-fluorescence of the peripheral zone of compact bone was also examined by microscopic spectrophotometry. Microradiographic examination revealed no morphological changes in bones left in the open air for long periods, except one of 15 years since death. In bones left in the soil, vacuoles of 5-10 microns diameter, which contained a honeycomb-like structure formed by small vacuoles of 0.5-1 microns diameter, were found in the peripheral zone of the substantia compacta approximately 5 years since death, and in bones of 6 years or more, this change extended to the mid-zone. In bones left in the sea for 4-5 years, vacuoles of 5-10 microns diameter were observed in the outer peripheral zone of the substantia compacta. The relative intensity of UV-fluorescence in bones dwindled with time since death and the correlation coefficient was considerably high.

Computer-assisted facial image identification system using a 3-D physiognomic range finder

This system consists of a 3-D physiognomic range finder and a computer-assisted facial image superimposition unit. The 3-D range finder is composed of a detector for measuring facial surface and its control computer. The detector has two sinusoidal grating projection devices and two CCD cameras. The computer-assisted facial image superimposition unit consists of a host computer including a proprietary software, a flat surface color display and a color image scanner for inputting 2-D facial images of a criminal. The 3-D facial shape and texture of a suspect is obtained by using the range finder. To make the comparison between the 3-D facial image and the 2-D facial image, the 3-D facial image is first reproduced on a display of the host computer from a MO disk and then the 2-D facial image is taken with the color image scanner and reproduced on the display. The 3-D facial image is exactly adjusted to match the orientation and size of the 2-D facial image under the fine framework mode, and then the fine framework mode of 3-D facial image is converted to the fine texture image. The shape and positional relationships of facial components between the 3-D and 2-D facial images are examined by the fade-out or wipe image mode. The distance between the selected two points and angle among the selected three points on the 3-D and 2-D facial images are automatically measured for the assessment of anthropometrical data between both images. For evaluating the fit between the anthropometrical points on the 3-D and 2-D facial images, the reciprocal point-to-point difference between both images is compared.

Evaluation of mRNA-based approach for identification of saliva and semen

Multiplex mRNA profiling by a reverse transcription-polymerase chain reaction (RT-PCR) has been reported in the last few years as a new approach for the identification of body fluids. We have also demonstrated the feasibility of identifying body fluids by using a real-time RT-PCR assay. Statherin (STATH) and histatin (HTN3), the selected genes for saliva, and protamin 2 (PRM2) and semenogelin 1 (SEMG1), those selected for semen, showed high specificity to these body fluids. Thus, the sensitivity and specificity of target genes were examined in body fluid stains. All target genes were detected in 0.1 microL 6-day-old stains, and showed high specificity in 7-day-old 30 microL stains. Furthermore, the stability of HTN3 in saliva stains was examined under various environmental conditions over time. The results showed that the degradation of mRNA in the stains was highly affected by wet conditions, and that light was also an important factor. However, mRNA was detectable in an older saliva stain (6 years old) and in an older semen stain (3.5 years old), both of which had been kept under dry and dark conditions. The stability of mRNA beyond our supposition may play an important role in developing new techniques for body fluid identification.

3-D imaging and quantitative comparison of human dentitions and simulated bite marks

This study presents a technique developed for 3-D imaging and quantitative comparison of human dentitions and simulated bite marks. A sample of 42 study models and the corresponding bites, made by the same subjects in acrylic dental wax, were digitised by laser scanning. This technique allows image comparison of a 3-D dentition with a 3-D bite mark, eliminating distortion due to perspective as experienced in conventional photography. Cartesian co-ordinates of a series of landmarks were used to describe the dentitions and bite marks, and a matrix was created to compare all possible combinations of matches and non-matches using cross-validation techniques. An algorithm, which estimated the probability of a dentition matching its corresponding bite mark, was developed. A receiver operating characteristic graph illustrated the relationship between values for specificity and sensitivity. This graph also showed for this sample that 15% of non-matches could not be distinguished from the true match, translating to a 15% probability of falsely convicting an innocent person.

Detecting hemifacial asymmetries in emotional expression with three-dimensional computerized image analysis.

Emotions are expressed more clearly on the left side of the face than the right: an asymmetry that probably stems from right hemisphere dominance for emotional expression (right hemisphere model). More controversially, it has been suggested that the left hemiface bias is stronger for negative emotions and weaker or reversed for positive emotions (valence model). We examined the veracity of the right hemisphere and valence models by measuring asymmetries in: (i) movement of the face; and (ii) observers rating of emotionality. The study uses a precise three–dimensional (3D) imaging technique to measure facial movement and to provide images that simultaneously capture the left or right hemifaces. Models (n = 16) with happy, sad and neutral expressions were digitally captured and manipulated. Comparison of the neutral and happy or sad images revealed greater movement of the left hemiface, regardless of the valence of the emotion, supporting the right hemisphere model. There was a trend, however, for left–sided movement to be more pronounced for negative than positive emotions. Participants (n = 357) reported that portraits rotated so that the left hemiface was featured, were more expressive of negative emotions whereas right hemiface portraits were more expressive for positive emotions, supporting the valence model. The effect of valence was moderated when the images were mirror–reversed. The data demonstrate that relatively small rotations of the head have a dramatic effect on the expression of positive and negative emotions. The fact that the effect of valence was not captured by the movement analysis demonstrates that subtle movements can have a strong effect on the expression of emotion.