Ming Tse Kao

Self-contained, biomolecular motor-driven protein sorting and concentrating in an ultrasensitive microfluidic chip.

We developed a molecular sorter that operates without external power or control by integrating the microtubule-based, biological motor kinesin into a microfluidic channel network to sort, transport, and concentrate molecules. In our devices, functionalized microtubules that capture analyte molecules are steered along kinesin-coated microchannel tracks toward a collector structure, concentrated, and trapped. Using fluorescent analyte molecules and nanoliter sample volumes, we demonstrated 14 fM sensitivity, even in the presence of high concentrations of other proteins.

Biomolecular motor-driven molecular sorter

We have developed a novel, microfabricated, stand-alone microfluidic device that can efficiently sort and concentrate (bio-)analyte molecules by using kinesin motors and microtubules as a chemo-mechanical transduction machine. The device removes hundreds of targeted molecules per second from an analyte stream by translocating functionalized microtubules with kinesin across the stream and concentrating them at a horseshoe-shaped collector. Target biomolecule concentrations increase up to three orders of magnitude within one hour of operation.

Biomolecular motor-driven microtubule translocation in the presence of shear flow: Analysis of redirection behaviours

We suggest a concept for powering microfluidic devices with biomolecular motors and microtubules to meet the demands for highly efficient microfluidic devices. However, to successfully implement such devices, we require methods for active control over the direction of microtubule translocation. While most previous work has employed largely microfabricated passive mechanical patterns designed to guide the direction of microtubules, in this paper we demonstrate that hydrodynamic shear flow can be used to align microtubules translocating on a kinesin-coated surface in a direction parallel to the fluid flow. Our evidence supports the hypothesis that the mechanism of microtubule redirection is simply that drag force induced by viscous shear bends the leading end of a microtubule, which may be cantilevered beyond its kinesin supports. This cantilevered end deflects towards the flow direction, until it is subsequently bound to additional kinesins; as translocation continues, the process repeats until the microtubule is largely aligned with the flow, to a limit determined by random fluctuations created by thermal energy. We present statistics on the rate of microtubule alignment versus various strengths of shear flow as well as concentrations of kinesin, and also investigate the effects of shear flow on the motility.

Surface landing of microtubule nanotracks influenced by lithographically patterned channels

Microtubules, which serve as cellular structural components in nature, can be placed within a lithographically patterned channel as engineered nanoscale tracks for bionanotechnology applications. We study the landing behavior of microtubules upon their diffusion onto a kinesin-coated glass surface in the presence of the channel. The influence of channel geometry on the landing rate of microtubules is experimentally characterized using channels with varying width. Additionally, we develop a theoretical model to quantitatively analyze our data by accounting for geometrical constraints due to both the width and height of the channels against the diffusion of the landing microtubules.