Mirela Noro

The use of eugenol against Aeromonas hydrophila and its effect on hematological and immunological parameters in silver catfish (Rhamdia quelen)

The aim of this study was to evaluate the activity of eugenol against the fish pathogen Aeromonas hydrophila and eugenols effect on hematological and natural immune parameters in silver catfish (Rhamdia quelen). In vitro, eugenol showed weak activity against A. hydrophila, but in vivo, at a subinhibitory concentration (10 mg L(-1)), it promoted survival in infected silver catfish. Eugenol (50 μg mL(-1)) reduced the hemolytic activity of A. hydrophila supernatant in vitro in fish erythrocytes. Subjecting catfish to eugenol baths (5 and 10 mg L(-1)) for five days did not alter the hematological and immunological parameters studied in this work. Based on these results, eugenol can be used to treat or prevent bacterial diseases in fish.

Hematologic values and blood biochemistry of anesthetized pudu (Pudu puda) in a semi-captive population

Southern pudu (Pudu puda) is one of the smallest deer in the world, categorized as vulnerable by the IUCN. Establishment of reference values for hematologic and biochemical guidelines is fundamental for monitoring health and physiological status of animals. The aim of this study was to describe ranges for hematological and plasma biochemical guidelines in pudu. Blood samples were collected from 29 anesthetized pudus (21 female and 8 males; 5 juveniles and 24 adults) from a semi-captive population. Hematologic parameters, plasma concentrations of energy, protein and mineral guidelines, as well activity of enzymes markers of hepatic and muscular damage were determined. Mean, median, and reference interval hematologic and biochemistry guidelines were presented. No significant differences between genders for any parameter were observed, except plasma calcium concentrations. The plasma concentrations of total protein, globulin, triacylglyceride, and chlorine were higher in adult than young pudus. Values obtained in this study will be employed to build a database for establishing reference limits for this species.