Mohamad Osman

Factors influencing malaysian public attitudes to agro-biotechnology

Despite considerable research in advanced countries on public perceptions of and attitudes to modern biotechnology, limited effort has been geared towards developing a structural model of public attitudes to modern biotechnology. The purpose of this paper is to identify the relevant factors influencing public attitudes towards genetically modified (GM) soybean, and to analyze the relationship between all the attitudinal factors. A survey was carried out on 1,017 respondents from various stakeholder groups in the Klang Valley region. Results of the survey have confirmed that attitudes towards complex issues such as biotechnology should be seen as a multifaceted process. The most important factors predicting support for GM soybean are the specific application-linked perceptions about the benefits, acceptance of risk and moral concern while risk and familiarity are significant predictors of benefit and risk acceptance. Attitudes towards GM soybean are also predicted by several general classes of attitude.

Screening of wild oil palm (Elaeis guineensis) germplasm for lipase activity

Palm oil comprises mainly triacylglycerols (TAG) that account for about 0·95 of its composition. Lipolysis of TAG releases free fatty acids that are then oxidized, impairing oil quality. Lower endogenous lipase activity is therefore desirable for better quality oil. Identifying genotypes for this should allow production of oils with improved shelf-life. A novel non-radioactive cold-activation titration assay was used to assay for lipase activity in 49 randomly selected Elaeis guineensis palms using the novel method as well as the traditional radioactive method. The results confirmed significant correlation between the radioactive and novel non-radioactive methods. A total of 148 E. guineensis palms originating from Angola, Cameroon, Guinea Conarky, Nigeria, Senagal, Sierra Leone, Tanzania and Zaire were then screened for lipase activity using the novel non-radioactive method. The analysis revealed genotypes with significantly different lipase activities, indicating that the activity is genotype-dependent. Quantitative polymerase chain reaction indicated similarity in pattern between gene expression and lipase activity determined by the novel assay. Several low and high lipase genotypes were identified. The findings pave the way for selection and breeding for low lipase genotypes. They are also relevant to genetic marker development for oil quality. Evidence is presented for the presence of at least two different lipases that determine oil quality in the oil palm mesocarp.

Improving plant growth and yield of jatropha (Jatropha curcas L.) through apical bud pinching

A field experiment was conducted to study the effect of the time of apical pinching and genotype on plant growth, flowering time, and yield of Jatropha curcas L. at the Universiti Kebangsaan Malaysia during 2008–2009. Three pinching treatments were used: no pinching or control (P0), pinching during transplanting (P1), and pinching at 30 days after transplanting (P2). Highly significant effect of time of pinching and genotype on plant growth and seed yield was observed. The plant height was significantly reduced by pinching the plant at 30 days after transplanting (72.4 cm) compared to pinching during transplanting (80.0 cm) and no pinching (95.5 cm). The maximum number of primary branches per plant (3.1) with more leaves per plant (113.2) and less plant height (72.2 cm) at first flowering was recorded with pinching at 30 days after transplanting. Among the treatments, unpinched plants produced flowers earlier than the pinched one. The highest number of fruits and seeds per plant along with higher seed yield was recorded in plants pinching at 30 days after transplanting. The maximum number of flowering branches was produced by genotype JC-14 followed by JC-18. The genotypes JC-13, JC-14, JC-17, JC-18, and JC-20 were found to be suitable in respect of reduced plant height; larger number of primary branches per plant; early flowering; and number of fruits, seeds, and seed yield per plant upon pinching.