Mohan Prasath Mani

Manufacturing and Characterization of Novel Electrospun Composite Comprising Polyurethane and Mustard Oil Scaffold with Enhanced Blood Compatibility

The objective of this work is to characterize and investigate the blood compatibility of polyurethane (PU)/mustard oil composites fabricated using electrospinning technique. The fabricated scaffold was characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), thermogravimetric analysis (TGA) and contact angle measurements. The activated partial thromboplastin time (APPT), prothrombin time (PT) and the hemolytic assay were done to investigate the blood compatibility of the developed composites. The SEM results revealed that the fiber diameter of the composites (761 ± 123 nm) was reduced compared to pristine PU control. The interaction between PU and mustard oil was confirmed by FTIR as evident through the shifting of peaks. The fabricated composites depicted hydrophobic behavior as insinuated by the increase in contact angle measurements. PU/mustard composites displayed improved crystallinity as confirmed by TGA. Atomic force micrographs suggested that developed PU/mustard oil composites showed an increase in the surface roughness (Ra) compared to pure PU. The Ra of pure PU was observed to be 723 nm but for the fabricated PU/mustard oil composite the Ra was found to be 1298 nm (Ra). The hemolytic index value for pure PU and fabricated composites was observed to be 2.73% and 1.15% indicating that developed composites showed a non-hemolytic behavior signifying the safety of the composites with red blood cells. Hence the newly developed composites with improved physicochemical and blood compatibility properties may be considered as a potential candidate for fabricating cardiac patches and grafts.

Appraisal of electrospun textile scaffold comprising polyurethane decorated with ginger nanofibers for wound healing applications

Nowadays, the synthetic biodegradable polymeric scaffolds were widely utilized for skin tissue engineering applications. The usage of nanofibrous scaffold manufactured through textile technologies has gained a widespread cynosure in wound healing applications. In this study, an electrospun textile scaffold based on polyurethane polymer incorporated with ginger extracts is hypothesized to possess unique properties facilitating wound healing. The fabricated electrospun membranes were characterized through scanning electron microscopy, Fourier transform infrared, thermogravimetric analysis, atomic force microscopy, and contact angle measurements. Coagulation assays such as activated prothrombin time, partial thromboplastin time, and hemolysis assay were performed for the electrospun membranes to determine their antithrombogenicity nature. Further, the cytocompatibility studies were evaluated using human dermal fibroblast cells to determine the toxicity of the electrospun polyurethane and their blends. Scanning electron microscopy revealed the reduced fiber (615 ± 154.31 nm) and pore (706 ± 90.07 nm) than the control (fiber diameter 1159 ± 147.48 nm and pore diameter 1087 ± 62.51 nm). Upon ginger incorporation, the wettability (80.33° ± 4.51) and surface roughness (389 nm) of the polyurethane was improved revealed in the contact angle and atomic force microscopy analysis. Infrared spectroscopy and thermogravimetric analysis study revealed the interaction of ginger with polyurethane by showing hydrogen bond formation and leaving an excess residue. The fabricated nanocomposites exhibited faster blood clotting time indicated in the activated prothrombin time and partial thromboplastin time. Further, hemolytic index and cytocompatibility studies revealed that the fabricated nanocomposites (Hemolytic index—0.96% and proliferation rate—159 ± 5.57%) showed nontoxic nature to the red blood cells and enhanced proliferation rate of human dermal fibroblast cells compared to the pristine polyurethane (hemolytic index—2.56% and proliferation rate—132 ± 3.61%). Our findings showed that polyurethane-based fibrous scaffolds comprising ginger exhibited desirable characteristics suitable for skin tissue engineering applications.

Single-stage electrospun innovative combination of polyurethane and neem oil: Synthesis, characterization and appraisal of blood compatibility

Wound healing is a complex process and it requires proper scaffolding for regeneration. An ideal scaffold should provide optimal environmental conditions in order to assist cellular attachment, proliferation and differentiation. In this work, a new composite based on polyurethane and neem oil was fabricated using one-step electrospinning technique. Fabricated composite patch along with the pristine polyurethane was characterized through scanning electron microscopy, Fourier transform and infrared spectroscopy, thermogravimetric analysis, contact angle measurement and atomic force microscopy. Moreover, the blood compatibility was evaluated using activated partial thromboplastin time, partial thromboplastin time and haemolysis assay. Scanning electron microscopy studies of composites revealed the existence of fibres with a smaller diameter (635  ± 105 nm) compared to the pristine polyurethane (969 ± 217 nm). Fourier transform and infrared analysis revealed the formation of hydrogen bond and peak shifting characteristics confirming the interaction of the neem oil with the polyurethane. Contact angle analysis showed the decrease in contact angle indicating the hydrophilic nature of the fabricated patch compared to pristine polyurethane. Thermal gravimetric analysis depicted the better thermal stability of the novel composite patch due to the existence of neem oil in the pristine polyurethane. The presence of neem oil in polyurethane matrix also resulted in an increase in the surface roughness as observed in the AFM analysis. The novel composite patch showed an ability to reduce the thrombogenicity and promoting the anticoagulant nature signified by blood compatibility assays like activated partial thromboplastin time and partial thromboplastin time. Finally, the haemolytic percentage of the fabricated composite (1%) was found to be reduced compared to control (2.733%) indicating better blood compatibility and safety with the red blood cells. Following the results, the fabricated patches offered enhanced physicochemical and blood compatible nature making it as a promising candidate for wound healing application.

Development and blood compatibility assessment of electrospun polyvinyl alcohol blended with metallocene polyethylene and plectranthus amboinicus (PVA/mPE/PA) for bone tissue engineering

Introduction Currently, the design of extracellular matrix (ECM) with nanoscale properties in bone tissue engineering is challenging. For bone tissue engineering, the ECM must have certain properties such as being nontoxic, highly porous, and should not cause foreign body reactions. Materials and methods In this study, the hybrid scaffold based on polyvinyl alcohol (PVA) blended with metallocene polyethylene (mPE) and plectranthus amboinicus (PA) was fabricated for bone tissue engineering via electrospinning. The fabricated hybrid nanocomposites were characterized by scanning electron microscopy (SEM), Fourier transform and infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), contact angle measurement, and atomic force microscopy (AFM). Furthermore, activated partial thromboplastin time (APTT), prothrombin time (PT), and hemolytic assays were used to investigate the blood compatibility of the prepared hybrid nanocomposites. Results The prepared hybrid nanocomposites showed reduced fiber diameter (238±45 nm) and also increased porosity (87%) with decreased pore diameter (340±86 nm) compared with pure PVA. The interactions between PVA, mPE, and PA were identified by the formation of the additional peaks as revealed in FTIR. Furthermore, the prepared hybrid nanocomposites showed a decreased contact angle of 51°±1.32° indicating a hydrophilic nature and exhibited lower thermal stability compared to pristine PVA. Moreover, the mechanical results revealed that the electrospun scaffold showed an improved tensile strength of 3.55±0.29 MPa compared with the pristine PVA (1.8±0.52 MPa). The prepared hybrid nanocomposites showed delayed blood clotting as noted in APTT and PT assays indicating better blood compatibility. Moreover, the hemolysis assay revealed that the hybrid nanocomposites exhibited a low hemolytic index of 0.6% compared with pure PVA, which was 1.6% suggesting the safety of the developed nanocomposite to red blood cells (RBCs). Conclusion The prepared nanocomposites exhibited better physico-chemical properties, sufficient porosity, mechanical strength, and blood compatibility, which favors it as a valuable candidate in bone tissue engineering for repairing the bone defects.

Surface, thermal and hemocompatible properties of novel single stage electrospun nanocomposites comprising polyurethane blended with bio oilTM

In this work, the physicochemical and blood compatibility properties of prepared PU/Bio oil nanocomposites were investigated. Scanning electron microscope (SEM) studies revealed the reduction of mean fiber diameter (709 ± 211 nm) compared to the pristine PU (969 nm ± 217 nm). Fourier transform infrared spectroscopy (FTIR) analysis exposed the characteristic peaks of pristine PU. Composite peak intensities were decreased insinuating the interaction of the bio oilTM with the PU. Contact angle analysis portrayed the hydrophobic nature of the fabricated patch compared to pristine PU. Thermal gravimetric analysis (TGA) depicted the better thermal stability of the novel nanocomposite patch and its different thermal behavior in contrast with the pristine PU. Atomic force microscopy (AFM) analysis revealed the increase in the surface roughness of the composite patch. Activated partial thromboplastin time (APTT) and prothrombin time (PT) signified the novel nanocomposite patch ability in reducing the thrombogenicity and promoting the anticoagulant nature. Finally the hemolytic percentage of the fabricated composite was in the acceptable range revealing its safety and compatibility with the red blood cells. To reinstate, the fabricated patch renders promising physicochemical and blood compatible nature making it a new putative candidate for wound healing application.