Morio Yamamoto
Kōchi University
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Publication
Featured researches published by Morio Yamamoto.
European Journal of Vascular and Endovascular Surgery | 2012
Morio Yamamoto; Kazumasa Orihashi; Hideaki Nishimori; Seiichiro Wariishi; Takashi Fukutomi; Nobuo Kondo; Kazuki Kihara; Takayuki Sato; Shiro Sasaguri
OBJECTIVES Indocyanine green (ICG) angiography is used for the intra-operative assessment of the graft vessel in coronary artery bypass grafting to enable immediate revision if necessary. We report the feasibility and implications of an ICG colour imaging system, HyperEye Medical System (HEMS), in surgeries for arteriosclerosis obliterans (ASO) and abdominal aortic aneurysm (AAA) which carry risk of mesenteric ischaemia. METHODS HEMS ICG angiography was used for the intra-operative assessment of 12 ASO patients and 10 AAA patients. RESULTS In the ASO patients, HEMS angiography enabled visualisation of the graft and native artery. The fluorescent lucent region in the artery distal to the anastomosis was shown in 1 of 12 ASO patients. There was a 3-s time lag in the increase of intensity between the proximal artery and distal stenotic region. In AAA patients, HEMS angiography clearly showed the perfusion in the mesenteric arteries and intestinal wall as opaque. One AAA patient had segmental ischaemia due to thromboembolism and another one had diffuse ischaemia due to systemic malperfusion. The ischaemic region of the intestine was visualised as a fluorescent lucent area by HEMS angiography. CONCLUSION HEMS angiography can accurately assess peripheral arterial perfusion in surgical cases with ASO and AAA.
Cell Transplantation | 2008
Nobuyuki Hirose; Hironori Maeda; Morio Yamamoto; Yoshihiro Hayashi; Gang-Hong Lee; Liyan Chen; Geethalakshmi Radhakrishnan; Parijatha Rao; Shiro Sasaguri
Macrophages play a pivotal role in the development of newly formed vascular networks, in addition to their normal immunological functions. This research focuses on peritoneal macrophages as a novel source in cell implantation therapy for ischemic diseases. In this study, production of angiogenic growth factors by peritoneal macrophages and its in vivo effect of neovascularization were evaluated. Mononuclear cells from the peritoneal cavity (P-MNCs) enriched with macrophages were isolated and stimulated with hypoxia and interleukin-1β (IL-1β) to mimic an ischemic tissue environment in vitro. Expression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) of mRNA in P-MNCs was apparently enhanced by hypoxic stimulation, and the production of VEGF protein was also augmented by hypoxia and IL-1β. A rat ischemic hind limb model was created and P-MNCs (8 × 106/limb) were injected into the ischemic muscles. The blood flow, which was assessed using the colored microsphere method, showed that the percentage blood flow was significantly increased by P-MNCs injection 4 weeks after surgery (48.3 ± 16.8% in noninjected ischemic limb vs. 84.3 ± 13.0% in the P-MNCs-injected limb). A histological analysis revealed that the number of capillaries detected by alkaline phosphatase staining was increased in the P-MNCs group 4 weeks after injection. Furthermore, the number of α-smooth muscle actin-positive vessels also showed a significant increase following P-MNC injection. The injected P-MNCs labeled with fluorescence were detected in the interstitial space of ischemic muscles, and VEGF protein expression of the implanted cells was confirmed by immunohistochemistry. These results indicate that peritoneal macrophages stimulate capillary formation and arteriogenesis in the ischemic limbs, possibly through the production of angiogenic growth factors. These findings suggest that the physiological angiogenic property of peritoneal macrophages could therefore be utilized for neovascularization in cell implantation therapy.
Biochemical and Biophysical Research Communications | 2009
Geethalakshmi Radhakrishnan; Morio Yamamoto; Hironori Maeda; Aimi Nakagawa; Rajesh KatareGopalrao; Hironobu Okada; Hideaki Nishimori; Seiichiro Wariishi; Eiji Toda; Hiroshi Ogawa; Shiro Sasaguri
Dissolved organic matter (DOM) in seawater can be defined as the fraction of organic matter that passes through a filter of sub micron pore size. In this study, we have examined the effect of DOM of deep seawater (DSW) from Pacific Ocean on platelet aggregation and atherosclerosis progression. DSW was passed through a series of filters and then through an Octadecyl C18 filter; the retained substance in ethanol was designated as C18 extractable DOM (C18-DOM). Our studies showed that C18-DOM treatment inhibited platelet aggregation, P-selectin expression and activity of COX-1 significantly. C18-DOM increased the expression of anti-atherogenic molecule namely heme oxygenase-1 in endothelial cells and all these data showed that C18-DOM is exhibiting aspirin-like effects. Moreover our in vivo studies showed that C18-DOM feeding slowed remarkably the progression of atherosclerosis. Our study demonstrated a novel biological effect of oceanic DOM, which has several important implications, including a possible therapeutic strategy for atherosclerosis.
American Journal of Transplantation | 2007
Morio Yamamoto; Hironori Maeda; Nobuyuki Hirose; Geethalakshmi Radhakrishnan; Rajesh Katare; Yoshihiro Hayashi; Parijatha Rao; Gang-Hong Lee; Tokio Yamaguchi; Shiro Sasaguri
Bilirubin, a strong intrinsic antioxidant, quenches free radicals produced under inflammatory conditions. The oxidized bilirubin metabolites, i.e. biopyrrins, are immediately excreted into urine and can indicate the intensity of oxidation in vivo. Our preliminary studies suggested the involvement of reactive nitrogen species (RNS) in generation of biopyrrins. However, little is known about biological significance of bilirubin oxidation by RNS. Here, we analyzed the correlation between bilirubin oxidation and nitric oxide (NO) radicals during rat acute cardiac allograft rejection. In allograft recipients, urinary biopyrrins steeply increased on day 3 prior to the increase in myocardial tissue damage marker, serum troponin‐T. In contrast, no significant changes in urinary biopyrrins were evident in recipients of isografts or cyclosporine‐A treated allografts. Urinary nitrotyrosine, a marker of oxidation by NO radicals also increased on day 3, while administration of a NO synthase inhibitor, NG‐monomethyl‐L‐arginine apparently diminished the elevation of urinary biopyrrins as well as nitrotyrosine. Immunohistochemistry revealed enhanced local expression of heme oxygenase‐1, biopyrrins and nitrotyrosine in allografts in accordance with the cellular infiltrates, suggesting that changes in urinary biopyrrins reflect the bilirubin oxidation in grafts undergoing rejection. These results indicate that locally evoked bilirubin oxidation by NO radicals can predict the progression of rejection.
Gastrointestinal Endoscopy | 2003
Shunji Mizobuchi; Kenshi Kuge; Hironori Maeda; Yasuhisa Matsumoto; Morio Yamamoto; Shiro Sasaguri
Transplantation Proceedings | 2005
Parijatha Rao; Hironori Maeda; X. Yutong; Morio Yamamoto; Nobuyuki Hirose; Shiro Sasaguri
Anticancer Research | 2005
Ryoko Suzuki; Rajesh Katare Gopalrao; Hironori Maeda; Parijatha Rao; Morio Yamamoto; Yutong Xing; Shunji Mizobuchi; Shiro Sasaguri
Japanese Circulation Journal-english Edition | 2008
Geethalakshmi Radhakrishnan; Ryoko Suzuki; Hironori Maeda; Morio Yamamoto; Nobuyuki Hirose; Rajesh Katare Gopalrao; Gang-Hang Lee; Yoshihiro Hayashi; Parijatha Rao; Shiro Sasaguri
American Surgeon | 2014
Tsutomu Namikawa; Uemura S; Nobuo Kondo; Morio Yamamoto; Hironori Maeda; Hideaki Nishimori; Takayuki Sato; Kazumasa Orihashi; Michiya Kobayashi; Kazuhiro Hanazaki
Circulation | 2008
Masaki Yamamoto; Hironori Maeda; Nobuyuki Hirose; Morio Yamamoto; Aimi Nakagawa; Geethalakshmi Radhakrishnan; Rajesh Katare Gopalrao; Takayuki Sato; Tokio Yamaguchi; Shiro Sasaguri