Motohiko Taki

Diazipine, a novel photoaffinity probe for dihydropyridine receptors of calcium channels

A new 1,4‐dihydropyridine photoaffinity ligand, [3H]diazipine, has been assessed by binding and photolabeling, and compared with a currently used [3H]Diazipine reversibly binds to skeletal muscle Ca2+ channels with a similar affinity of [3H]azidopine, but [3H]diazipine labels the channels two times more efficiently and no release of the incorporated amount is observed after dithiothreitol treatment.

Synthesis and Properties of Diazipine, a Novel Photoaffinity Probe for Calcium Channel-Linked Dihydropyridine Receptors

Photoaffinity labeling techniques using reagents with ligand structures have versatile roles in picking up and identifying receptor molecules of the ligands in crude preparations, and even in locating or identifying the binding sites of the ligands in the receptor molecules.