Muhammad Anjum Aqueel

Virulence of Verticillium lecanii (Z.) against cereal aphids; does timing of infection affect the performance of parasitoids and predators?

BACKGROUND Entomopathogenic fungi such as Verticillium lecanii (Z.) (Mycotal(®)) are used for pest control as an alternative to chemical control. In this study, the effect of V. lecanii on cereal aphids is assessed. In addition, an investigation is carried out to determine whether the use of V. lecanii affects the performance of two natural enemies of aphids, the predator Harmonia axyridis (P.) and the parasitoid Aphidius colemani (V.), in no-choice experiments under laboratory conditions. RESULTS The number of Rhopalosiphum padi (L.) and Sitobion avenae (F.) killed was increased by increasing the concentration of V. lecanii. The timing of application of fungus to aphids affected the efficacy of other biocontrol agents, a parasitoid and a predator. Parasitation by A. colemani (V.) in both cereal aphids (S. avenae and R. padi) was not affected by V. lecanii when aphids were first treated with V. lecanii and then exposed to A. colemani. The emergence of adults from parasitised mummies was, however, lower in infected aphids than in uninfected aphids when the aphids were first exposed to the parasitoids and then treated with fungus. The female sex ratio in the emerging adults was lower in V. lecanii-treated aphids in both species. When aphids were first treated with V. lecanii, 72 h before predation, fewer aphids of both species were consumed by H. axyridis (P.). CONCLUSION Use of entomopathogenic fungus as a biological control agent could be a complementary strategy in an integrated pest management programme against cereal aphids, but it can reduce the efficiency of other biocontrol agents (parasitoids and predators) when applied simultaneously.

Tritrophic interactions between parasitoids and cereal aphids are mediated by nitrogen fertilizer

Host plant nutritional quality can directly and indirectly affect the third trophic levels. The aphid–parasitoid relationship provides an ideal system to investigate tritrophic interactions (as the parasitoids are completely dependent for their development upon their hosts) and assess the bottom up forces operating at different concentrations of nitrogen applications. The effects of varying nitrogen fertilizer on the performance of Aphidius colemani (V.) reared on Sitobion avenae (F.) and Aphidius rhopalosiphi (D.) reared on Rhopalosiphum padi (L.) were measured. Parasitism and percent emergence of parasitoids were positively affected by nitrogen fertilizer treatments while developmental duration (egg, larval, and pupal stages) was not affected by increasing nitrogen inputs. In males and females of both parasitoid species, adult longevity increased with the increasing nitrogen fertilizer. Hind tibia length and mummy weight of both parasitoid species increased with nitrogen fertilizer concentrations, as a result of larger aphids. This study showed that nitrogen application to the soil can have important consequences for aboveground multitrophic interactions.

Biochemical studies on the amylase of Mango Mealybug (Drosicha stebbingi Green)

Summary Mealybug (Drosicha stebbingi Green) is voracious pest of the mango orchards. It causes heavy quality losses and low production of mango fruit (Luhanga & Gwinner 1993). The research was conducted to control the pest. Isolation, purification and characterization of amylase from mango mealybug were performed. Amylolytic activity was 365U/ml in crude homogenate of D. stebbingi whereas in acetone precipitation sample activity was 680 U/ml. The optimum pH and temperature for amylase activity was 6.4 and 50-60 oC, respectively. However, amylase activity was maximum at 3% of substrate (starch) concentration. Enzymatic activity was inhibited by Pb