Muhammad Nawaz Chaudhry

Application of ozone in cotton bleaching with multiple reuse of a water bath

This paper discusses the effect of ozone bleaching on the quality of cotton fabric in terms of whiteness, dye pick-up, copper number and degree of polymerization (DP). The process parameters (pH, ozone dose and treatment time) were optimized, and the best whiteness (60–62) and quality of bleached fabric was obtained at pH 5, 45 min treatment time and room temperature. A strongly acidic environment and longer ozonation time decreased the quality of fabric by producing a higher copper number (0.18) and DP loss (51.5%). Statistical analysis of the experimental data using analysis of variance confirmed that process parameters significantly affected the bleaching efficiency. The process was then applied to bleach 20 different samples of cotton fabric re-using the same water bath, and the whiteness of ozone-bleached samples did not decrease, even after the 20th reuse of the water bath.

Mutations of the human interferon alpha-2b (hIFN-α2b) gene in occupationally protracted low dose radiation exposed personnel

Ionizing radiations impact human tissues by affecting the DNA bases which constitute genes. Human interferon alpha 2b gene synthesizes a protein which is an important anticancerous, immunomodulatory, anti-proliferative and antiviral protein. This study was aimed to identify interferon alpha-2b mutations as a consequence of the use of occupational chronic low dose radiation by hospital radiation exposed workers. A molecular analysis was done in which DNAs were extracted from blood samples from radiology, radiotherapy and nuclear medicine workers. The gene was amplified through polymerase chain reaction and further genetic data from sequencing results analyzed by bioinformatics tools in order to determine as to how mutations in interferon alpha 2b sequences will lead to changes in human interferon alpha-2b protein. A total of 41% gene mutations was detected among all radiation exposed workers in which higher percentage (5.4%) of base insertion mutations and 14% frameshift mutations were found in radiology workers. The chronic use of low dose of radiations by occupational workers has a significant correlation with mutational effects on interferon alpha 2b gene, further evident by depressed interferon alpha levels in serum. This can lead to depressed immunity in radiation exposed workers. Hematological profiling of this group also showed hyperimmune response in the form of lymphocytosis.

Synthesis, characterization and photocatalytic activity of Al2O3-TiO2 based composites

The synthesis, characterization and photocatalytic performance of non-traditional Al2O3-TiO2-based photocatalysts is reported. Al2O3-TiO2 support was loaded with various fractions of CuO and ZrO2. A sound agreement was observed between the bandgaps of synthesized powders measured by UV-Visible diffuse reflectance spectroscopy (DRS) in the solid phase and UV-Visible spectroscopy in the aqueous medium. X-ray diffraction (XRD) analysis revealed the composite nature of the catalysts with the retention of individual identity of each component. The average crystallite size of the individual component was found to be in the range of 20 to 40 nm. Scanning electron microscopy (SEM) analysis authenticated the presence of CuO and ZrO2 at the surface of Al2O3-TiO2 support, while Rutherford Back Scattering Spectroscopy (RBS) confirmed the quantity of the modifiers as per theoretical calculations. The composites showed an enhanced photocatalytic activity in sunlight compared to Al2O3-TiO2 for the degradation of dyes. Efforts were made to elucidate the enhanced sunlight response of the synthesized composite catalysts compared to Al2O3-TiO2. As monitored by ion chromatography (IC), the synthesized photocatalysts completely mineralized the dyes leaving behind inorganic ions in solution. The kinetics of photocatalytic degradation of dyes was evaluated for optimum correlation with the existing models. The stability of the photocatalysts against the photo-corrosion was monitored by analyzing the samples for respective metals in solution after sunlight exposure. Supplemental materials are available for this article. Go to the publishers online edition of the Journal of Environmental Science and Health, Part A, to view the supplemental file.

A Pilot-Scale Application of Ozone to Bleach Raw Cotton Fabric Using Various Additives

The influence of various additives on the efficiency of optimized ozone bleaching process at pilot scale has been discussed in this study. The results reveal that the best whiteness (63.79) and strength of bleached fabric is achieved with the addition of surfactant (2 g/L) at an ozone dose of 50 g/h, pH 5, and ozone treatment time of 45 min at room temperature. The dyeing quality of ozone-bleached and hydrogen peroxide-bleached fabric samples is almost identical. The analysis of variance of the experimental data validates that the process parameters have significantly affected the efficiency of ozone bleaching process.

Assessment of key parameters in municipal solid waste management: a prerequisite for sustainability

Municipal solid waste management was studied for 1 year in a representative urban area of Lahore city for environmental sustainability. The effects of financial status of the household and the seasonal variations on generation rate and compositions of municipal solid waste (MSW) were determined. MSW generation rate and the economic status of the households were positively correlated (p < 0.05). Seasonal variations observed were significant only for organics (p = 0.001), plastics (p = 0.008) and food waste fractions (p = 0.009) in MSW. Response surface regression model developed and analysed by Minitab-15® showed that the interaction of season and different economic zones of the town on the MSW generation rate was non-significant (p = 0.334). Elemental and heating value analyses of the mixed organic fractions in kitchen waste had carbon 47.93%, hydrogen 6.20%, nitrogen 2.24%, sulphur 0.23%, oxygen 39.01%, and C and N ratio 27.78. Findings concluded that food waste was 56% of total MSW with 71.03% moisture content and a modest heating value of 5566 J/g. Existing temporary storage capacity of MSW is 51% of the total MSW generated considering the weighted MSW generation rate of 0.57 kg/person/day calculated in this study. Composting could be a possible final disposal option due to high moisture and organic content and can be studied in future research. Development of a transfer station, introduction of home composting programmes and awareness towards proper segregation and reduction of waste at the household level is suggested to attain sustainability in the MSW management system.

Sludge granulation and efficiency of phase separator in UASB reactor treating combined industrial effluent

Sludge granulation and the effect of gas-liquid-solid separator (GLSS) design on the efficiency of upflow anaerobic sludge blanket (UASB) and upflow anaerobic sludge filter (UASF) reactors, operating at HRTs ranging from 3 to 12 h were investigated. VSS/TS ratio gradually increased in both the reactors with increasing sludge age (from 0.5 to more than 0.7 for UASB reactor and 0.012 to 0.043 for UASF reactor). X-Ray diffraction analysis of the UASF sludge showed the presence of expanding clays revealing its additional absorption capability. Fuoraphyllite and albite precipitation related to excellular polymers of the microbial shell structure, showed the extended growth of microorganisms during sludge granulation. A gradual decrease (82%-69%) in COD removal with decreasing HRT was apparent in UASF reactor. In case of UASB reactor, this decrease was marginal because addition of GLSS device significantly improved (14%-20%) the overall efficiency of the UASB reactor. GLSS enhanced the efficiency of the UASB reactor by increasing the settleability of suspended particles and accelerating the coagulation of colloidal particles due to the velocity gradient.

Estimation of residual antibiotics in soil and underground water of areas affected by pharmaceutical wastewater in Lahore

Drinking water is most essential thing for life on earth. Due to environmental pollution day by day the quality and quantity of drinking water is lowering. Ground water tables are becoming contaminated due industrial pollution. Contamination level of antibiotics namely ofloxacin (OFL), ciprofloxacin (CIP), levofloxacin (LEV), oxytetracycline (OTC), doxycycline (DOX) were quantified in soil and underground water of surrounding areas of pharmaceutical industry in Lahore. HPLC with DAD detector, C-18 column and solid phase cartridges were used to analyze antibiotic residues. In groundwater DOX has highest mean level of contamination and OFL was determined as highest contaminating pollutant in soil. Highest detected value in groundwater of OFL was 0.50, CIP and LEV 0.20, DOX 0.80, OTC 0.40 ng/L. Ground water have residual level of CIP 0.01–0.20 ng/L, LEV 0.02–0.20 ng/L, OFL 0.01–0.50 ng/L, OTC 0.02–0.40 ng/L, and DOX 0.04–0.80 ng/L. Ground water was contaminated by these residues is very important as it is source of accumulation of antibiotics to human and animal body as well as to plants.

Studying bio-thermal effects at and around MSW dumps using Satellite Remote Sensing and GIS

Estimating negative impacts of MSW dumps on its surrounding environment is the key requirement for any remedial measures. This study has been undertaken to map bio-thermal effects of MSW dumping at and around dumping facilities (non-engineered) using satellite imagery for Faisalabad, Pakistan. Thirty images of Landsat 8 have been selected after validation for the accuracy of their observational details from April 2013 to October 2015. Land Surface Temperature (LST), NDVI, SAVI and MSAVI have been derived from these images through Digital Image Processing (DIP) and have been subjected to spatio-temporal analysis in GIS environment. MSW dump has been found with average temperature elevation of 4.3K and 2.78K from nearby agriculture land and urban settlement respectively. Vegetation health has been used as the bio-indicator of MSW effects and is implemented through NDVI, SAVI, MSAVI. Spatial analyses have been used to mark boundary of bio-thermally affected zone around dumped MSW and measure 700m. Seasonal fluctuations of elevated temperatures and boundary of the bio-thermally affected zones have also been discussed. Based on the direct relation found between vegetation vigor and the level of deterioration within the bio-thermally affected region, use of crops with heavy vigor is recommended to study MSW hazard influence using bio-indicators of vegetation health.

Mutations of the human interferon alpha-2b (hIFNα-2b) gene in low-dose natural terrestrial ionizing radiation exposed dwellers.

Natural terrestrial ionizing radiations emerge from uranium deposits and can impact human tissues by affecting DNA bases which constitute genes. Human interferon alpha-2b (hIFNα-2b) gene synthesizes a protein which exhibits anticancerous, immunomodulatory, anti-proliferative and antiviral properties. This research aimed to find out hIFNα-2b gene mutations for those residents who were chronically exposed to low-dose natural terrestrial ionizing radiations. The gene amplifications was done through PCR technique and gene mutations were identified by bioinformatics in order to conclude as to how mutations identified in hIFNα-2b gene sequences will lead to alterations in the hIFNα-2b protein in radiation exposed residents. The range of radiation dose exposure was 0.4383-4.55832 (mSv/y) for the selected radiation exposed locations which were having uranium mineralization. Mutations (24%) in hIFNα-2b gene shows that some of the radiation exposed inhabitants were having a modulated immune response. The CBC (Complete Blood Count) parameters: WBC (White Blood Cells), MCH (Mean Corpuscular Hemoglobin), MCHC (MCH Concentration) and PLT (Platelets) on average were below the normal range in 24% radiation exposed subjects who were having hIFNα-2b gene mutations. Immunomodulation is observed by the mixed trend of either lymphocytosis or lymphopenia and neutropenia or neutrophilia in the exposed population. Thus, a radioactive exposure from uranium can affect the immune system and can induce mutations.

Sequence analysis of the rifampicin resistance determining region (RRDR) of rpoB gene in multidrug resistance confirmed and newly diagnosed tuberculosis patients of Punjab, Pakistan

Molecular screening of new patients suspected for TB could help in the effective control of TB in Pakistan as it is a high TB burden country. It will be informative to understand the prevalence of multi drug resistance for a better drug regimen management in this geographical area. The Rifampicin resistance determining region (RRDR) sequencing was used to identify mutations associated with drug resistance in DNA extracts from 130 known multidrug resistant (MDR) cultured strains and compared with mutations observed in DNA extracts directly from 86 sputum samples from consecutive newly diagnosed cases in Lahore, Pakistan. These newly diagnosed samples were positive for smear microscopy, chest X-ray and presumed sensitive to first line drugs. In the known MDR group the most frequent mutations conferring resistance were found in rpoB531 (n = 51, 39.2%). In the newly diagnosed tuberculosis group with no history of MDR, mutations in rpoB531 were seen in 10 of the samples (11.6%). Collectively, all mutations in the RRDR region studied were observed in 80 (61.5%) of known MDR cases and in 14 (16.3%) of the newly diagnosed cases. Using the RRDR as a surrogate marker for MDR, sequences for the newly diagnosed (presumed sensitive) group indicate much higher levels of MDR than the 3.9% WHO 2015 global estimate and suggests that molecular screening directly from sputum is urgently required to effectively address the detection and treatment gaps to combat MDR in this high burden country.