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Featured researches published by Muqing Zhang.


PLOS ONE | 2014

Species-specific detection and identification of fusarium species complex, the causal agent of sugarcane pokkah boeng in China.

Zhenyue Lin; Shiqiang Xu; Youxiong Que; Jihua Wang; Jack C. Comstock; Jinjin Wei; Per H. McCord; Baoshan Chen; Rukai Chen; Muqing Zhang

Background Pokkah boeng disease caused by the Fusarium species complex results in significant yield losses in sugarcane. Thus, the rapid and accurate detection and identification of the pathogen is urgently required to manage and prevent the spreading of sugarcane pokkah boeng. Methods A total of 101 isolates were recovered from the pokkah boeng samples collected from five major sugarcane production areas in China throughout 2012 and 2013. The causal pathogen was identified by morphological observation, pathogenicity test, and phylogenetic analysis based on the fungus-conserved rDNA-ITS. Species-specific TaqMan real-time PCR and conventional PCR methods were developed for rapid and accurate detection of the causal agent of sugarcane pokkah boeng. The specificity and sensitivity of PCR assay were also evaluated on a total of 84 isolates of Fusarium from China and several isolates from other fungal pathogens of Sporisorium scitamineum and Phoma sp. and sugarcane endophyte of Acremonium sp. Result Two Fusarium species (F. verticillioides and F. proliferatum) that caused sugarcane pokahh boeng were identified by morphological observation, pathogenicity test, and phylogenetic analysis. Species-specific TaqMan PCR and conventional PCR were designed and optimized to target their rDNA-ITS regions. The sensitivity of the TaqMan PCR was approximately 10 pg of fungal DNA input, which was 1,000-fold over conventional PCR, and successfully detected pokkah boeng in the field-grown sugarcane. Conclusions/Significance This study was the first to identify two species, F. verticillioides and F. proliferatum, that were causal pathogens of sugarcane pokkah boeng in China. It also described the development of a species-specific PCR assay to detect and confirm these pathogens in sugarcane plants from mainland China. This method will be very useful for a broad range of research endeavors as well as the regulatory response and management of sugarcane pokkah boeng.


Sugar Tech | 2008

Cloning and identification of promoter Prd29A and its application in sugarcane drought resistance

Y. Wu; H. Zhou; Youxiong Que; Rukai Chen; Muqing Zhang

The 420 bp upstream regulatory region of rd29A gene from Arabidopsis thaliana genome was amplified by PCR. Sequence analysis showed that it shared 100% identity with the reported rd29A promoter and contained several cis-acting elements including dehydration responsive elements (DRE), ABA responsive elements (ABRE) and so on. The plant expression vector prd29A-S65T was constructed with this fragment linked up with green fluorescent protein (GFP) gene controlled by rd29A promoter, which was transferred to sugarcane callus by bombardment. The expressions of gfp in the transformed sugarcane callus and leaves treated with 6g/L PEG and 40% PEG, respectively, were detected by fluorescent microscope. The results showed that the expression vector had been transferred into sugarcane callus and GFP gene could be induced by PEG treatment. Through PCR analysis, 5 plants were identified to be the transgenic. At the same time, plant expression vector rd29a-dreb-hyg was constructed with this fragment linked up with DREB2B gene controlled by inducible promoter rd29A, which will lay the foundation for droughtresistant transgenic breeding in sugarcane. Molecular analysis showed that the rd29a-dreb-hyg has been successfully integrated into corresponding sugarcane variety.


PLOS ONE | 2015

Characterization of Chromosome Inheritance of the Intergeneric BC2 and BC3 Progeny between Saccharum spp. and Erianthus arundinaceus.

Yongji Huang; Jiayun Wu; Ping Wang; Yanquan Lin; Cheng Fu; Zuhu Deng; Qinnan Wang; Qiwei Li; Rukai Chen; Muqing Zhang

Erianthus arundinaceus (E. arundinaceus) has many desirable agronomic traits for sugarcane improvement, such as high biomass, vigor, rationing ability, tolerance to drought, and water logging, as well as resistance to pests and disease. To investigate the introgression of the E. arundinaceus genome into sugarcane in the higher generations, intergeneric BC2 and BC3 progeny generated between Saccharum spp. and E. arundinaceus were studied using the genomic in situ hybridization (GISH) technique. The results showed that the BC2 and BC3 generations resulted from n + n chromosome transmission. Furthermore, chromosome translocation occurred at terminal fragments from the E. arundinaceus chromosome in some progeny of Saccharum spp. and E. arundinaceus. Notably, the translocated chromosomes could be stably transmitted to their progeny. This study illustrates the characterization of chromosome inheritance of the intergeneric BC2 and BC3 progeny between Saccharum spp. and E. arundinaceus. This work could provide more useful molecular cytogenetic information for the germplasm resources of E. arundinaceus, and may promote further understanding of the germplasm resources of E. arundinaceus for sugarcane breeders to accelerate its progress in sugarcane commercial breeding.


PLOS ONE | 2014

Unexpected Inheritance Pattern of Erianthus arundinaceus Chromosomes in the Intergeneric Progeny between Saccharum spp. and Erianthus arundinaceus

Jiayun Wu; Yongji Huang; Yanquan Lin; Cheng Fu; Shaomou Liu; Zuhu Deng; Qiwei Li; Zhongxing Huang; Rukai Chen; Muqing Zhang

Erianthus arundinaceus is a valuable source of agronomic traits for sugarcane improvement such as ratoonability, biomass, vigor, tolerance to drought and water logging, as well as resistance to pests and disease. To investigate the introgression of the E. arundinaceus genome into sugarcane, five intergeneric F1 hybrids between S. officinarum and E. arundinaceus and 13 of their BC1 progeny were studied using the genomic in situ hybridization (GISH) technique. In doing so, we assessed the chromosome composition and chromosome transmission in these plants. All F1 hybrids were aneuploidy, containing either 28 or 29 E. arundinaceus chromosomes. The number of E. arundinaceus chromosomes in nine of the BC1 progeny was less than or equal to 29. Unexpectedly, the number of E. arundinaceus chromosomes in the other four BC1 progeny was above 29, which was more than in their F1 female parents. This is the first cytogenetic evidence for an unexpected inheritance pattern of E. arundinaceus chromosomes in sugarcane. We pointed to several mechanisms that may be involved in generating more than 2n gametes in the BC1 progeny. Furthermore, the implication of these results for sugarcane breeding programs was discussed.


Sugar Tech | 2008

Establishment of genetic transformation system and obtaining transgenic sugarcane (var. badila) transformed with RS gene

Liping Xu; Youxiong Que; Jingsheng Xu; S. R. Fang; Muqing Zhang; Yun Chen; Rukai Chen

Resveratrol Synthase (RS) is one of the key enzymes in resveratrol biosynthesis, which catalyzes one molecule of coumaroyl CoA and three molecules of malonyl CoA to form one molecule of resveratrol. In this study, the genetic transformation system for badila, a chewing cane variety, was established. The optimized media for regeneration were as follows: induction medium was MS+2,4-D 2mg.L−1; differentiation medium was MS+NAA 0.2 mg.L−1+BA 2 mg.L−1; rooting medium was 1/2 MS+NAA 2 mg.L−1. The addition of 0.5 % activated carbon in the differentiation and rooting medium could decrease the phenol poison and promote rooting. At the same time, the optimal concentration of G418 in subculture and differentiation period was determined as: 30 mgL−1 for differentiation and 25 mgL−1 for rooting. RS gene was transferred into badila via gene gun bombardment through the constructed plant expression vector pBIL-RS. PCR detection and southern blot analysis showed that seven putative transgenic plants had been obtained. Therefore, the genetic transformation system of badila had been elementarily established and proved to be efficient.


In Vitro Cellular & Developmental Biology – Plant | 2015

Effective selection and regeneration of transgenic sugarcane plants using positive selection system

Muqing Zhang; Xiaolei Zhuo; Jihua Wang; Yang Wu; Wei Yao; Rukai Chen

We compared the transformation frequency of a positive selection system, which employs an Escherichia coli-derived pmi (phosphomannose isomerase) gene as the selectable marker and mannose as the selective agent, to commonly used negative selection systems of bar (phosphinothricin acetyltransferase) /phosphinothricin (PPT), npt II (neomycin phosphotransferase) /G418 (geneticin), and hpt (hygromycin phosphotransferase) /Hygromycin. Transgenic sugarcane plants were obtained following biolistic transformation of callus with four different selectable marker genes. Under different selective agents, transformed callus was selectively proliferated on MS medium containing 13.57xa0μM of 2,4-D, regenerated on MS medium with 4.44xa0μM of BA, 9.29xa0μM of KT, and 0.89xa0μM of NAA, and rooted on half of MS medium with 13.38xa0μM of NAA and 0.44xa0μM of 6-BA. The results demonstrated that the transformation frequency of 4.2% scored in the pmi/mannose positive system was significantly higher than those in the negative selection systems (0.47% for bar/PPT, 1.38% for npt II/G418, and 0.63% for hpt/Hygromycin, respectively). Transgenic sugarcane plants using the pmi/mannose system were confirmed by polymerase chain reaction (PCR) analysis and chlorophenol red (CPR) assay. The CPR assay was found to efficiently and qualitatively detect the transgenic sugarcane from the nontransformed plants because medium color changes in the CPR assay were highly responsive to pmi gene expression and enzyme activity. Thus, a pmi/mannose positive selection system for sugarcane transformation was developed to obviate the use of negative selection systems based on herbicides and antibiotics.


Plant Disease | 2014

First report of Phoma sp. causing twisting and curling of crown leaves of sugarcane in mainland of China.

Zhenyue Lin; Youxiong Que; Zuhu Deng; Shiqiang Xu; G. P. Rao; Muqing Zhang

Sugarcane is a major sugar and the leading energy crop worldwide and Guangxi is the largest sugarcane production area in China (2011 Sugar Annual China, www.gain.fas.usda.gov). During survey of sugarcane crops in September 2012 and June 2013, ~5 to 10% of sugarcane (cvs. FN-40 and ROC22) planted in Chongzuo and Laibing, Guangxi Zhuang Autonomous Region, P. R. China, had twisted and curling symptoms of crown leaves similar to sugarcane Pokkah boeng disease (caused by Fusarium moniliforme Sheldon). The symptoms started appearing as yellowing on midribs and leaf margins that spread further to the entire leaf, along with twisting and curling of crown leaves. The symptomatic leaf tissues (5 × 5 mm) were surface-sterilized by 0.1% HgCl2 solution for 30 s, followed by rinsing three times in sterile water, placed on potato dextrose agar (PDA), and then incubated in darkness at 28°C. After 3 days of incubation, the isolated fungal colony appeared as white villous, spherical, radial, and dense colorless mycelium from the top, while it was reddish-brown at the bottom and later became grayish. Chlamydospores were also observed with a diameter of 5 to 10 μm and were dark brown, unicellular, intercalary, and smooth. The binucleate hyphae were colorless and transparent. Pycnidia appeared on the colonies after 20 days, and were dark brown, subglobose, and 150 to 230 μm in diameter, and the conidia were ~3 to 7 × 2.5 to 6 μm, unicellular, colorless, and ovoid to oval. The fungal isolates from the symptomatic leaves were obtained and pathogenicity was evaluated. Conidial suspensions (107 CFU/ml) of the single isolate from FN-40 were micro-injected into 20 sugarcane seedlings of cultivar FN-40. Another 20 seedlings were injected with water without conidia as control. The inoculated plants were grown in a growth chamber at 28°C with a 16-h photoperiod. Twisted and curly symptoms similar to the field appeared on the inoculated leaves at 10 days after inoculation, while the control leaves remained asymptomatic. The fungus was re-isolated and identified. Genomic DNA from the cultured fungal isolate was extracted with a modified Fungal DNA Midi Kit (Omega Bio-Tek, Inc., Norcross, GA), and amplified using fungus-conserved primer sequences (ITS1: 5-TCCGTAGGTGAACCTGCGG-3 and ITS4: 5-TCCTCCGCTTATTGATATGC-3). The consensus rDNA-internal transcribed spacer sequence (GenBank Accession No. KC524502) was 100% identical with 97% coverage to the ITS sequence from Phoma sp. 3. TMS-2011 (HQ631000.1) in GenBank (3). The fungus Phoma sp. was identified on the basis of morphological characteristics (2,4) and the ITS sequence of rDNA (1,3). Disease caused by Phoma sp. has been reported earlier on sugarcane from Pakistan, Hawaii, and Taiwan, causing leaf blight and curling (2,4). However, to the best of our knowledge, this is the first report of Phoma sp. causing twisting and curling of crown leaves of sugarcane in mainland of China. References: (1) M. M. Aveskamp et al. Stud. Mycol. 65:1, 2010. (2) A. Sanguino and H. Tokeski. ISSCT Proc. 17:1555, 1980. (3) P. Shrestha et al. Appl. Environ. Microbiol. 77:5490, 2011. (4) Z. N. Wang. Rep. Taiwan Sugar Res. Inst. 129:1, 1980.


Agricultural Sciences in China | 2010

Analysis of Disequilibrium Hybridization in Hybrid and Backcross Progenies of Saccharum officinarum × Erianthus arundinaceus

Zuhu Deng; Muqing Zhang; Wei-le Lin; Fu Cheng; Chui-ming Zhang; Yu-chang Li; Li-ping Lai; Yanquan Lin; Rukai Chen

Erianthus arundinaceus is an important, closely related genus of Saccharum officinarum L. It is therefore important to understand how the chromosomes are transmitted when it hybridizes with sugarcane. The hybrids and backcross progenies of S. officinarum and E. arundinaceus and their parents were used for Karyotype analysis and to study the law of chromosome transmission. The results showed that the somatic chromosome number of both of the E. arundinaceus Hainan92-105 and Hainan92-77 were 2n = 60 = 60sm, belonging to type 1A, and the BC1 YC01-21 was 2n = 104 = 100m + 4sm, belonging to type 2C. The other six tested clones belonged to type 2B. The both F1s YC96-66 and YC96-40 that originated from Badila (2n = 80 = 70m + 10sm) with E. Arundinaceus were 2n = 70 = 68m + 2sm, which suggests an n + n transmission. The cross between YC96-66 (female parent) and CP84-1198 (male parent, 2n = 120 = 114m + 6sm) also followed the same genetic law and the somatic chromosome number of their progeny, YC01-3 (2n = 105 = 95m + 10sm). The cross derived from YC96-40 (female) and CP84-1198 (male), YC01-21 had 2n = 104 = 100m + 4sm chromosomes, following the same genetic law of n + n. However, YC01-36 had 2n = 132 = 130m + 2sm chromosomes, which suggests a 2n + n chromosome transmission. It can be inferred that the inheritance of chromosomes was very complex in the BC1. The difference in chromosome number between clones was as high as 28. This could be explained by the 2n + n transmission of chromosomes. In addition, as there was not be a regular number of haploids, this phenomenon is termed as disequilibrium hybridization.


Archive | 2008

Method for quickly acquiring transgenic sugarcane by using pmi gene

Muqing Zhang; Yang Wu; Rukai Chen; Xiaolei Zhuo


Plant Disease | 2017

First Report of Sugarcane Leaf Chlorotic Streak Disease Caused by Xanthomonas sacchari in Guangxi, China

H. J. Sun; J. J. Wei; Y. S. Li; Y. X. Bao; Y. P. Cui; Y. Z. Huang; H. Zhou; R. Z. Yang; G. P. Rao; Muqing Zhang

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Rukai Chen

Fujian Agriculture and Forestry University

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Youxiong Que

Fujian Agriculture and Forestry University

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Zhenyue Lin

Fujian Agriculture and Forestry University

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Yang Wu

Fujian Agriculture and Forestry University

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Yanquan Lin

Fujian Agriculture and Forestry University

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Zuhu Deng

Fujian Agriculture and Forestry University

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Jiayun Wu

Fujian Agriculture and Forestry University

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