Mutsuo Murata

Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction

Bone morphogenetic protein (BMP) was extracted from bone matrix, dentin matrix, and wound tissue after tooth extraction in rabbits, and purified. These purified fractions were shown to be homogeneous by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis (SDS-PAGE), and induced new bone in situ in 3 weeks when implanted into the calf muscles of Wistar rats. The dentin matrix-derived BMP was different from the other two types in molecular weight and the properties revealed in the process of purification. However, each tissue-derived BMP was shown to induce new bone growth in a bioassay of xenogenic implantation. For this reason, BMP is thought to have subunits with certain commonalities in different tissue.

Purification of bone morphogenetic protein derived from bovine bone matrix.

Bone morphogenetic protein (BMP) was extracted from the bovine bone matrix and purified by liquid chromatography. The molecular weight of the BMP was 18 kDa by SDS-PAGE, and its pI value was 4.9. Amino acid analysis suggested that the BMP is a polypeptide containing 163 amino acids. In the present study, telopeptide-free type I collagen was used as a carrier of BMP.

Destructive calcium pyrophosphate dihydrate temporo-mandibular arthropathy (pseudogout)

A case of a 57-year-old man with destructive calcium pyrophosphate dihydrate (CPPD) arthropathy (pseudogout) together with pseudotumor formation in the temporomandibular joint (TMJ).

Marked gingival hyperplasia induced by nifedipine

Abstract An unusual case is reported of gingival hyperplasia associated with the use of nifedipine.

Aggressive infantile fibromatosis of the submandibular region

Aggressive infantile fibromatosis is an unusual, tumor-like, proliferative lesion which grows infiltratively. The clinical and histological features of aggressive infantile fibromatosis in the submandibular region of a 3-year-old girl are reported.

Monostotic fibrous dysplasia with involvement of the mandibular canal

A 72-year-old woman sought treatment with a rare monostotic fibrous dysplasia occurring in the mandible and involving the mandibular canal. Her chief complaint was swelling, which had appeared about 2 years previously, had enlarged gradually, and was associated with spontaneous pain. X-ray film examination revealed a ground-glass opaque image of blurred demarcation, and 99mTc-methylene diphosphonate bone scintigraphy disclosed an area of marked radioisotope uptake. Histopathologic examination revealed the area of bone marrow and spongy bone to be replaced by fibrous tissue, irregular beam-shaped woven bone, and lamellar bone. Contouring of the expanded portion of the bone and surgical decompression of the mandibular canal were performed with good results.

A case of malignant fibrous histiocytoma of the right maxilla - 2. The observation of electron microscopic studies.

A case of malignant fibrous histiocytoma was encountered which developed a tumor (5.0×4.5×4.0 cm) extending from the right maxillary region to the maxillary sinus region of a 60-year-old woman. By electron-microscopic observation, this tumor was composed of fibrouslike cells, histiocyte-like cells and multi-nuclear giant cells in varying propotions according to the excised regions, some parts of which were bleeding. In this tumor, xanthoma-like cells, which have been observed by many research workers, were not evident, as far as we could see. But it is noteworthy that unknown multi nuclear giant cells with many granules and vacuoles were recognized.