Toshirou Tagawa

Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction

Bone morphogenetic protein (BMP) was extracted from bone matrix, dentin matrix, and wound tissue after tooth extraction in rabbits, and purified. These purified fractions were shown to be homogeneous by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis (SDS-PAGE), and induced new bone in situ in 3 weeks when implanted into the calf muscles of Wistar rats. The dentin matrix-derived BMP was different from the other two types in molecular weight and the properties revealed in the process of purification. However, each tissue-derived BMP was shown to induce new bone growth in a bioassay of xenogenic implantation. For this reason, BMP is thought to have subunits with certain commonalities in different tissue.

Purification of bone morphogenetic protein derived from bovine bone matrix.

Bone morphogenetic protein (BMP) was extracted from the bovine bone matrix and purified by liquid chromatography. The molecular weight of the BMP was 18 kDa by SDS-PAGE, and its pI value was 4.9. Amino acid analysis suggested that the BMP is a polypeptide containing 163 amino acids. In the present study, telopeptide-free type I collagen was used as a carrier of BMP.

Destructive calcium pyrophosphate dihydrate temporo-mandibular arthropathy (pseudogout)

A case of a 57-year-old man with destructive calcium pyrophosphate dihydrate (CPPD) arthropathy (pseudogout) together with pseudotumor formation in the temporomandibular joint (TMJ).

Acanthosis nigricans with oral lesions and a malignant visceral tumor: A case report

Abstract Acanthosis nigricans (AN) is a disease characterized by papillary proliferation of the mucosa and skin, hyperkeratosis, and pigmentation. It is classified as malignant, benign, or pseudo types.1−5 In the malignant type, mucosal and dermal signs precede or accompany malignant visceral tumors.2,4,5 Thus, recognition of AN may possibly facilitate the diagnosis of the malignancy. However, AN is rarely reported in the oral region. This article reports a case of AN with oral papillomatous lesions and a ma ant visceral tumor.

Characterization of four cell lines derived from a human malignant fibrous histiocytoma of the maxillary sinus.

We have established four cell lines from a human malignant fibrous histiocytoma. Each cell line had human aneuploid karyotype and DNA aneuploidy. Cells in all lines expressed CD13, CD68 and vimentin but lacked CD11, CD14, CD15, CD16, CD45, HLA class II and other mesenchymal and epithelial markers such as desmin, alpha-smooth muscle, myoglobin, S-100 protein, and cytokeratin. None of the cells expressed surface IgG or C3 receptor, nor did any of them phagocytose latex particles. The cells reacted with an antibody for prolyl-4-hydroxylase, but no collagen (types I, II, III, or IV) was detected in any of the cell lines. The homogenates of all cell lines had cyclic nucleotide phosphodiesterase 3 activity. Two cell lines produced granulocyte colony-stimulating factor (G-CSF), interleukin (IL)-1alpha, IL-6 and tumor necrosis factor alpha, another line produced G-CSF, IL-1alpha, IL-6 and platelet-derived growth factor (PDGF)-AB, and the remaining cell line produced only PDGF-AB. None of the cells produced transforming growth factor-alpha. The results indicated that the cell lines were immunophenotypically similar to primitive mesenchymal cells.

Monostotic fibrous dysplasia with involvement of the mandibular canal

A 72-year-old woman sought treatment with a rare monostotic fibrous dysplasia occurring in the mandible and involving the mandibular canal. Her chief complaint was swelling, which had appeared about 2 years previously, had enlarged gradually, and was associated with spontaneous pain. X-ray film examination revealed a ground-glass opaque image of blurred demarcation, and 99mTc-methylene diphosphonate bone scintigraphy disclosed an area of marked radioisotope uptake. Histopathologic examination revealed the area of bone marrow and spongy bone to be replaced by fibrous tissue, irregular beam-shaped woven bone, and lamellar bone. Contouring of the expanded portion of the bone and surgical decompression of the mandibular canal were performed with good results.