Myoung-Hoon Jeong

Enhancement of Anticancer Activity of Acer mono by High Pressure Extraction Process

We investigated a method to improve anticancer activities of Acer mono by ultra high pressure extraction process. The extract yields by ultra high pressure were 9.49% and 9.87% for 5 min and 15 min processing time, respectively, which were relatively higher than 34% of conventional extraction processes due to their resid bark structure. The extract for 15 minutes extraction (HPE15) showed higher potent scavenging effect as 94.56% than the control, BHA as 93.24%. On SOD-like test, HPE15 also showed the highest activity as 38.6% at 1.0 mg/mL concentration. The cytotoxicity of HPE15 on normal human lung and kidney cell were below 23.54% in adding 1.0 mg/mL. Generally, human cancer cell growth stomach adenocarcinoma (AGS), lung adenocarcinoma (A549), breast adenocarcinoma (MCF-7), colon adenocarcinoma (Caco-2) and liver adenocarcinoma (Hep3B) were inhibited up to 75% with higher selectivity of above 4.0. High antioxidant activity of HPE15 resulted in high anticancer activity, and its activity was also due to higher yields of Acer mono by ultra high pressure extraction process. It was also proved by HPLC comparison analysis.

Enhancement of Whitening Effects of Lithospermum erythrorhizon Extracts by Ultra High Pressure

In this study, whitening activity of Lithospermum erythrorhizon extracts were investigated according to several extraction processes: water extraction at (WE100) and (WE60), 70% ethyl alcohol extraction (EE) and ultra high pressure extraction (HPE) at 500 MPa for 30 minutes at . The extracts from ultra high pressure extraction showed the highest tyrosinase inhibition and melanogenesis inhibition activities as 52% and 79.5%, respectively, in adding than others extraction processes. HPE extracts also showed the strong reducing power as 3.19 that absorbance at 450 mm. The contents of polyphenol in WE100, we measured as in adding . Extracts have a high total flavonoid contents by HPE as at . We can conclude that better whitening activity of extracts from high pressure extraction was due to high antioxidant activities which could be extracted by higher polyphenol and flavonoid contents in HPE than others.