N. Reis

Shear Stress, Temperature, and Inoculation Concentration Influence the Adhesion of Water-Stressed Helicobacter pylori to Stainless Steel 304 and Polypropylene

ABSTRACT Although molecular techniques have identified Helicobacter pylori in drinking water-associated biofilms, there is a lack of studies reporting what factors affect the attachment of the bacterium to plumbing materials. Therefore, the adhesion of H. pylori suspended in distilled water to stainless steel 304 (SS304) coupons placed on tissue culture plates subjected to different environmental conditions was monitored. The extent of adhesion was evaluated for different water exposure times, using epifluorescence microscopy to count total cell numbers. High shear stresses—estimated through computational fluid dynamics—negatively influenced the adhesion of H. pylori to the substrata (P < 0.001), a result that was confirmed in similar experiments with polypropylene (P < 0.05). However, the temperature and inoculation concentration appeared to have no effect on adhesion (P > 0.05). After 2 hours, H. pylori cells appeared to be isolated on the surface of SS304 and were able to form small aggregates with longer exposure times. However, the formation of a three-dimensional structure was only very rarely observed. This study suggests that the detection of the pathogen in well water described by other authors can be related to the increased ability of H. pylori to integrate into biofilms under conditions of low shear stress. It will also allow a more rational selection of locations to perform molecular or plate culture analysis for the detection of H. pylori in drinking water-associated biofilms.

Application of a novel oscillatory flow micro-bioreactor to the production of γ-decalactone in a two immiscible liquid phase medium

A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of γ-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred tank bioreactor or shake flask). A three-fold increase in γ-decalactone productivity was obtained by increasing oscillatory mixing intensity from Reo ~482 to Reo ~1447. This was presumably related to the effective contribution of the reactor geometry to enhanced mass transfer rates between the two immiscible liquid phases involved in the process by increasing the interfacial area.

CFD simulations of RTD of a strawberry pulp in a continuous ohmic heater

Abstract A pilot ohmic heater is to be tested for the continuous aseptic processing of strawberry pulps and jams. The hydrodynamics and the fluid residence time distribution (RTD) have been experimentally investigated for Newtonian and non-Newtonian fluids (water and an industrial strawberry pulp, respectively), for several inlet flow rates. The results were obtained using Computational Fluid Dynamics (CFD) with a user-defined function (UDF) description of the fluid phase (pulp). For all of the conditions tested the fluid phase is described using the laminar flow model. The results show that the RTD is affected by the inlet flow rate but not so significantly by the process temperature. Some shortcuts and dead zones were detected in the ohmic heater specially for Newtonian fluids. The heater behaves like a piston flow with longitudinal mixing.