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Dive into the research topics where N. V. Shishkanova is active.

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Featured researches published by N. V. Shishkanova.


Fems Yeast Research | 2003

Oxygen requirements for growth and citric acid production of Yarrowia lipolytica

Svetlana V. Kamzolova; N. V. Shishkanova; Igor G. Morgunov; T. V. Finogenova

During continuous cultivation of Yarrowia lipolytica N 1, oxygen requirements for growth and citric acid synthesis were found to depend on the iron concentration in the medium. A coupled effect of oxygen and iron concentrations on the functioning of the mitochondrial electron transport chain in Y. lipolytica N 1 was established. Based on the results obtained in continuous culture, conditions for citric acid production in a batch culture of Y. lipolytica N 1 were proposed. At relatively low pO(2) value and a high iron concentration, citric acid accumulation was as high as 120 g l(-1); the specific rate of citric acid synthesis reached 120 mg citric acid (g cells h)(-1). The mass yield coefficient was 0.87 and the energy yield coefficient was 0.31.


Microbiology | 2003

Biochemical characterization of the yeast Yarrowia lipolytica overproducing carboxylic acids from ethanol. Nitrogen metabolism enzymes

A. P. Il'chenko; Cherniavskaia Og; N. V. Shishkanova; T. V. Finogenova

A comparative assay of nitrogen metabolism enzymes in the Yarrowia lipolytica mutant N1 grown under conditions promoting the overproduction of either α-ketoglutaric acid (KGA) or citric acid showed that the overproduction of KGA correlates with an increase in the activities of the NAD- and NADP-linked glutamate dehydrogenase, glutamic–pyruvic transaminase, and glutamic–oxaloacetic transaminase reactions. These reactions are likely to be responsible for the overproduction of KGA by this mutant. In contrast, the overproduction of citric acid correlated with a decline in the activities of the NAD- and NADP-linked glutamate dehydrogenases and with an increase in the activities of glutamine synthetase and glutamate synthase.


Bulletin of Experimental Biology and Medicine | 1996

Effect of ethanol concentration on the maximal specific growth rate and biomass composition ofYarrowia lipolytica mutant strain No. 1

Svetlana V. Kamzolova; T. I. Chistyakova; E. G. Dedyukhina; N. V. Shishkanova; T. V. Finogenova

Abstractthe effect of ethanol concentration on the maximal specific growth rate and biomass composition ofYarrowia lipolytica No. 1 was studied during culturing in the pH-auxostat mode. Growth inhibition set in starting from a 2.64 g/liter residual concentration of ethanol. The constant of ethanol inhibition was 11.0 g/liter. Growth inhibition with ethanol was associated with changes of the fatty-acid composition of lipids and a resultant reduction of lipid unsaturation.


Microbiology | 2003

The Induction of Cytochrome P-450 and Ethanol Oxidation in Yarrowia lipolytica Cells

A. P. Il'chenko; O. G. Chernyavskaya; N. V. Shishkanova; T. V. Finogenova

The study of the effect of different ethanol concentrations in the medium on the growth and activity of enzymatic systems involved in ethanol oxidation in Yarrowia lipolytica showed that the cultivation of yeast cells on 1 and 2% ethanol caused their rapid growth and a drastic increase in cell respiration and sensitivity to cyanide already in the first hours of cultivation. At the same time, during cultivation on 3, 4, and 5% ethanol, the growth and respiration of yeast cells were considerably suppressed. All of the ethanol concentrations studied induced the synthesis of cytochrome P-450, its dynamics in cells being dependent on the initial concentration of ethanol in the medium. When the initial concentration of ethanol was 1 and 2%, the content of cytochrome P-450 in cells steeply decreased after a short period of induction. However, when the initial concentration of ethanol in the medium was 4 to 5%, the content of cytochrome P-450 in cells was high throughout the cultivation period. The induction of cytochrome P-450 in cells preceded the induction of the NAD-dependent enzymes alcohol dehydrogenase and catalase, which, like cytochrome P-450, are also involved in ethanol oxidation by yeasts. The activity of catalase was higher in the yeast cells grown in the presence of 3 to 5% ethanol than in the cells grown in the presence of 1 and 2% ethanol. The roles played by cytochrome P-450, alcohol dehydrogenase, and catalase in ethanol oxidation by yeast cells are discussed.


Food Technology and Biotechnology | 2005

Lipase Secretion and Citric Acid Production in Yarrowia lipolytica Yeast Grown on Animal and Vegetable Fat

Svetlana V. Kamzolova; Igor G. Morgunov; Andreas Aurich; Oksana A. Perevoznikova; N. V. Shishkanova; Ulrich Stottmeister; Tatiana V. Finogenova


Process Biochemistry | 2004

Pyruvic acid production by a thiamine auxotroph of Yarrowia lipolytica

Igor G. Morgunov; Svetlana V. Kamzolova; Oksana A. Perevoznikova; N. V. Shishkanova; Tatiana V. Finogenova


Applied Microbiology and Biotechnology | 2002

Biosynthesis of citric and isocitric acids from ethanol by mutant Yarrowia lipolytica N 1 under continuous cultivation

T. V. Finogenova; Svetlana V. Kamzolova; E. G. Dedyukhina; N. V. Shishkanova; A. P. Il'chenko; Igor G. Morgunov; O. G. Chernyavskaya; Sokolov Ap


Microbiology | 1998

Oxygen concentration as a factor controlling central metabolism and citric acid biosynthesis in the yeast Yarrowia lipolytica grown on ethanol

A. P. Il'chenko; N. V. Shishkanova; O. G. Chernyavskaya; T. V. Finogenova


Microbiology | 1996

Effects of temperature, pH, and ethanol concentration on the maximal specific growth rate and biomass composition of Yarrowia lipolytica, mutant strain no. 1

Svetlana V. Kamzolova; T. I. Chistyakova; E. G. Dedyukhina; N. V. Shishkanova; T. V. Finogenova


Microbiology | 2001

[Metabolic characteristics of the Yarrowia lipolytica strain producing alpha-ketoglutaric and citric acids from ethanol and the effect of [NH4+] and [O2] on yeast respiration and biosynthesis].

A. P. Il'chenko; Cherniavskaia Og; N. V. Shishkanova; T. V. Finogenova

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T. V. Finogenova

Russian Academy of Sciences

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A. P. Il'chenko

Russian Academy of Sciences

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Igor G. Morgunov

Russian Academy of Sciences

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Cherniavskaia Og

Russian Academy of Sciences

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E. G. Dedyukhina

Russian Academy of Sciences

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T. I. Chistyakova

Russian Academy of Sciences

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