Na Duan

Conversion efficiency and oil quality of low-lipid high-protein and high-lipid low-protein microalgae via hydrothermal liquefaction.

Hydrothermal liquefaction (HTL) is a promising technology for converting algae into biocrude oil. Here, HTL of a low-lipid high-protein microalgae (Nannochloropsis sp.) and a high-lipid low-protein microalgae (Chlorella sp.) was studied. An orthogonal design was applied to investigate the effects of reaction temperature (220-300°C), retention time (30-90 min), and total solid content (TS, 15-25%wt) of the feedstock. The highest biocrude yield for Nannochloropsis sp. was 55% at 260°C, 60 min and 25%wt, and for Chlorella sp. was 82.9% at 220°C, 90 min and 25%wt. The maximum higher heating values (HHV) of biocrude oil from both algae were ∼ 37 MJ/kg. GC-MS revealed a various distribution of chemical compounds in biocrude. In particular, the highest hydrocarbons content was 29.8% and 17.9% for Nannochloropsis and Chlorella sp., respectively. This study suggests that algae composition greatly influences oil yield and quality, but may not be in similar effects.

Hydrothermal liquefaction of harvested high-ash low-lipid algal biomass from Dianchi Lake: effects of operational parameters and relations of products.

Hydrothermal liquefaction (HTL) allows a direct conversion of algal biomass into biocrude oil, not only solving the environmental issues caused by the over-growing algae but also producing renewable energy. This study reports HTL of algae after separation from eutrophicated Dianchi Lake in China. Conversion efficiency was studied under different operational conditions via an orthogonal design, including holding temperature (HT) (260-340 °C), retention time (RT) (30-90 min) and total solid (TS) (10-20%). A highest biocrude oil yield (18.4%, dry ash-free basis, daf) was achieved at 300 °C, 60 min, and 20% (TS), due to the low contents of lipids (1.9%, daf) and proteins (24.8%, daf), and high contents of ash (41.6%, dry basis) and carbohydrates (71.8%, daf). Operational parameters significantly affected the biocrude yields, and chemical distribution of HTL products. The biocrude production also related to other HTL products, and involved chemical reactions, such as deoxygenation and/or denitrogenation.

Recovery of reducing sugars and volatile fatty acids from cornstalk at different hydrothermal treatment severity

This study focused on the degradation of cornstalk and recovery of reducing sugars and volatile fatty acids (VFAs) at different hydrothermal treatment severity (HTS) (4.17-8.28, 190-320°C). The highest recovery of reducing sugars and VFAs reached 92.39% of aqueous products, equal to 34.79% based on dry biomass (HTS, 6.31). GC-MS and HPLC identified that the aqueous contained furfural (0.35-2.88 g/L) and 5-hydroxymethyl furfural (0-0.85 g/L) besides reducing sugars and VFAs. Hemicellulose and cellulose were completely degraded at a HTS of 5.70 and 7.60, respectively. SEM analysis showed that cornstalk was gradually changed from rigid and highly ordered fibrils to molten and grainy structure as HTS increased. FT-IR and TGA revealed the significant changes of organic groups for cornstalk before and after hydrothermal treatment at different HTS. Hydrothermal treatment might be promising for providing feedstocks suitable for biohythane production.

Co-digestion of chicken manure and microalgae Chlorella 1067 grown in the recycled digestate: Nutrients reuse and biogas enhancement

The present investigation targeted on a sustainable co-digestion system: microalgae Chlorella 1067 (Ch. 1067) was cultivated in chicken manure (CM) based digestate and then algae biomass was used as co-substrate for anaerobic digestion with CM. About 91% of the total nitrogen and 86% of the soluble organics in the digestate were recycled after the microalgae cultivation. The methane potential of co-digestion was evaluated by varying CM to Ch. 1067 ratios (0:10, 2:8, 4:6, 6:4, 8:2, 10:0 based on the volatile solids (VS)). All the co-digestion trials showed higher methane production than the calculated values, indicating synergy between the two substrates. Modified Gompertz model showed that co-digestion had more effective methane production rate and shorter lag phase. Co-digestion (8:2) achieved the highest methane production of 238.71mL⋅(g VS)-1 and the most significant synergistic effect. The co-digestion (e.g. 8:2) presented higher and balanced content of dominant acidogenic bacteria (Firmicutes, Bacteroidetes, Proteobacterias and Spirochaetae). In addition, the archaea community Methanosaeta presented higher content than Methanosarcina, which accounted for the higher methane production. These findings indicated that the system could provide a practicable strategy for effectively recycling digestate and enhancing biogas production simultaneously.

Improved methane production and energy recovery of post-hydrothermal liquefaction waste water via integration of zeolite adsorption and anaerobic digestion

Hydrothermal liquefaction (HTL) is a promising technology for converting organic wastes into bio-crude oil, with organic-rich post-hydrothermal liquefaction wastewater (PHWW) as by-product. In this study, zeolite adsorption and anaerobic digestion (AD) were integrated to improve the methane production and energy recovery of PHWW from Chlorella 1067. A statistical design for maximum toxicants removal by zeolite was applied before AD process. Zeolite could mitigate the inhibition associated to compounds such as ammonia, N-heterocyclic compounds, etc. in PHWW and thereby shortening the lag phase and increasing methane production by 32-117% compared with that without zeolite adsorption. Zeolite adsorption also increased energy recovery efficiency (up to 70.5%) for this integrated system. Integration of HTL and AD brought higher energetic return from feedstock via oil and biomethane production, which may offer insight into industrial application of microalgae biomass in the circular economy. In addition, carbon and nitrogen flow for the integrated process was determined.

Inhibitors degradation and microbial response during continuous anaerobic conversion of hydrothermal liquefaction wastewater

One critical challenge of hydrothermal liquefaction (HTL) is its complex aqueous product, which has a high concentration of organic pollutants (up to 100gCOD/L) and diverse fermentation inhibitors, such as furfural, phenolics and N-heterocyclic compounds. Here we report continuous anaerobic digestion of HTL wastewater via an up-flow anaerobic sludge bed reactor (UASB) and packed bed reactor (PBR). Specifically, we investigated the transformation of fermentation inhibitors and microbial response. GC-MS identified the complete degradation of furfural and 5-hydroxymethylfurfural (5-HMF), and partial degradation (54.0-74.6%) of organic nitrogen and phenolic compounds, including 3-hydroxypyridine, phenol and 4-ethyl-phenol. Illumina MiSeq sequencing revealed that the bacteria families related to detoxification increased in response to the HTL aqueous phase. In addition, the increase of acetate-oxidizing bacteria in UASB and acetogens in PBR showed a strengthened acetogenesis. As for the archaeal communities, an increase in hydrogenotrophic methanogens was observed. Based on GC-MS/HPLC and microbial analysis, we speculate that dominant fermentation inhibitors were transformed into intermediates (Acetyl-CoA and acetate), further contributing to biomethane formation.

Biogas liquid digestate grown Chlorella sp. for biocrude oil production via hydrothermal liquefaction

Microalgae can not only purify and recover the nutrients from wastewater, but also be harvested as wet biomass for the production of biocrude oil via hydrothermal liquefaction (HTL). Chlorella sp. cultivated in the ultrafiltration (UF) membrane treated anaerobic digestion (AD) liquid digestate of chicken manure was used as the feedstock in this study. The present study characterized the products and investigated the elemental migration during HTL of Chlorella sp. fed with AD effluent wastewater (WW) and BG11 standard medium (ST) in 100mL and 500mL reactors under different operational conditions. Results showed that the highest oil yield of WW (38.1%, daf) was achieved at 320°C, 60min and 15% TS in 500mL reactor, which was 14.1% higher than that of ST (33.4%, daf) at 320°C, 30min and 20% TS in the same reactor. WW had a similar carbon and hydrogen distribution in the four product fractions under HTL conditions compared with ST. 43.4% and 32.4% of carbon in WW11 and ST11 were released into the biocrude and aqueous phase in 500mL reactor, respectively. As much as 64.5% of the hydrogen was transferred to the aqueous phase. GC-MS results showed that the chemical compounds in the biocrude oil from WW consist of a variety of chemical constituents, such as hydrocarbons, acids, alcohols, ketones, phenols and aldehydes. These two biocrude oils contained 17.5% wt. and 8.64% wt. hydrocarbons, and 63.7% wt. and 79.8% wt. oxygen-containing compounds, respectively. TGA results showed that 69.3%-66.7% of the biocrude oil was gasified in 30°C-400°C. This study demonstrates the great potential for biocrude oil production from microalgae grown in biogas effluent via HTL.

Microalgae cultivation and culture medium recycling by a two-stage cultivation system

Nutrients and water play an important role in microalgae cultivation. Using wastewater as a culture medium is a promising alternative to recycle nutrients and water, and for further developing microalgae-based products. In the present study, two species of microalgae, Chlorella sp. (high ammonia nitrogen tolerance) and Spirulina platensis (S. platensis, high growth rate), were cultured by using poultry wastewater through a two-stage cultivation system for algal biomass production. Ultrafiltration (UF) or centrifuge was used to harvest Chlorella sp. from the first cultivation stage and to recycle culture medium for S. platensis growth in the second cultivation stage. Results showed the two-stage cultivation system produced high microalgae biomass including 0.39 g·L–1Chlorella sp. and 3.45 g·L–1S. platensis in the first-stage and second-stage, respectively. In addition, the removal efficiencies of NH4+ reached 19% and almost 100% in the first and the second stage, respectively. Total phosphorus (TP) removal reached 17% and 83%, and total organic carbon (TOC) removal reached 55% and 72% in the first and the second stage, respectively. UF and centrifuge can recycle 96.8% and 100% water, respectively. This study provides a new method for the combined of pure microalgae cultivation and wastewater treatment with culture medium recycling.

Temporal changes in the characteristics of algae in Dianchi Lake, Yunnan Province, China

Algal blooms have become a worldwide environmental concern due to water eutrophication. Dianchi Lake in Yunnan Province, China is suffering from severe eutrophication and is listed in the Three Important Lakes Restoration Act of China. Hydrothermal liquefaction allows a promising and direct conversion of algal biomass into biocrude oil. In this study, algal samples were collected from Dianchi Lake after a separation procedure including dissolved air flotation with polyalu- minum chloride and centrifugation during four months, April, June, August and October. The algal biochemical components varied over the period; lipids from 0.7% to 2.1% ash-free dry weight (afdw), protein from 20.9% to 33.4% afdw and ash from 36.6% to 45.2% dry weight. The algae in June had the highest lipid and protein concentra- tions, leading to a maximum biocrude oil yield of 24.3% afdw. Biodiversity analysis using pyrosequencing revealed different distributions of microbial communities, specifi- cally Microcystis in April (89.0%), June (63.7%) and August (84.0%), and Synechococcus in April (2.2%), June (12.0%) and August (1.0%). This study demonstrated remarkable temporal changes in the biochemical composi- tion and biodiversity of algae harvested from Dianchi Lake and changes in biocrude oil production potential.