Nájla Mohamad Kassab

Development and validation of UV spectrophotometric method for determination of levofloxacin in pharmaceutical dosage forms

The objective of this research was to develop and validate an alternative analytical method for quantitative determination of levofloxacin in tablets and injection preparations. The calibration curves were linear over a concentration range from 3.0 to 8.0 μg mL-1. The relative standard deviation was below 1.0% for both formulations and average recovery was 101.42 ± 0.45% and 100.34 ± 0.85% for tablets and injection formulations, respectively. The limit of detection and limit of quantitation were 0.08 and 0.25 μg mL-1, respectively. It was concluded that the developed method is suitable for the quality control of levofloxacin in pharmaceuticals formulations.

Stability-indicating HPLC-DAD method for the simultaneous determination of fluoroquinolones and corticosteroids in ophthalmic formulations

The aim of this study is to develop and validate a stability-indicating assay method for simultaneous determination of gatifloxacin and prednisolone acetate, or of ciprofloxacin hydrochloride and dexamethasone in combination and in the presence of degradation products. Reverse-phase high-performance liquid chromatography is used. All analyses were carried out on a Kinetex C18 column and acetronitrile–water (50 : 50 v/v) pH 3.0 mobile phase with 0.30 mL min−1 flow rate. Efficient chromatographic separation of these drugs and their forced degradation products is achieved in less than 6 min and with a peak purity match factor higher than 950. The method shows linearity in the concentration range of 1.2 to 9.6 μg mL−1 for gatifloxacin (r = 0.9995), 2.0 to 16.0 μg mL−1 for prednisolone acetate (r = 0.9997), 2.5 to 25.0 μg mL−1 for both ciprofloxacin hydrochloride (r = 0.9993) and dexamethasone (r = 0.9998), precision (relative standard deviation lower than 2%), accuracy (mean recovery 100 ± 2%), and robustness, according to ICH and AOAC guidelines. This method is able to determine simultaneous ophthalmic combinations of these drugs and to separate the drug peaks from their forced degradation products. Additionally, the optimized chromatographic conditions can contribute to minimize waste of organic solvent .

A Critical Review of Properties and Analytical Methods for the Determination of Oxytetracyline in Biological and Pharmaceutical Matrices

ABSTRACT Antibiotics have an unquestionable importance in the treatment of many infections. Oxytetracycline is an antibiotic belonging to the class of tetracyclines, available for use in human and veterinary medicine. Development of analytical methods that prove the quality and efficacy of these drugs is fundamentally important to the pharmaceutical industry. In this context, the research presents an overview of the analytical profile of oxytetracycline, describing its chemical and pharmacological properties, and analytical methods for quantification of this drug in biological samples and pharmaceutical products. Oxytetracycline can be analyzed in these matrices by many types of methodologies. However, high-performance liquid chromatography is the most widely used, being recommended by official compendia. This kind of study can be useful to support the development of new efficient and sustainable analytical methods that may be utilized in the quality control routine of oxytetracycline in pharmaceutical products and pharmacokinetic monitoring in biological samples.

Design, Synthesis and Anticancer Biological Evaluation of Novel 1,4-Diaryl- 1,2,3-triazole Retinoid Analogues of Tamibarotene (AM80)

We report herein the design and synthesis via click chemistry of twelve novel triazole retinoid analogues of tamibarotene (AM80) and the evaluation of their anticancer activities against six cancer cell lines: HL60, K562, 786, HT29, MCF7 and PC3. Among the synthesized compounds, two were more potent than tamibarotene against solid tumor cells, and one of them had similar potency to tamibarotene against HL60 cells. The bioisosteric exchange between the amide group and the 1,2,3-triazole core in the retinoid agent tamibarotene (AM80) reported in this work is a valid strategy for the generation of useful compounds against cancer.