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Dive into the research topics where Naoko Honda is active.

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Featured researches published by Naoko Honda.


Journal of Clinical Microbiology | 2011

Multilocus Sequence Typing Implicates Rodents as the Main Reservoir Host of Human-Pathogenic Borrelia garinii in Japan

Ai Takano; Minoru Nakao; Toshiyuki Masuzawa; Nobuhiro Takada; Yasuhiro Yano; Fubito Ishiguro; Hiromi Fujita; Takuya Ito; Xiao-Hang Ma; Yozaburo Oikawa; Fumihiko Kawamori; Kunihiko Kumagai; Toshiyuki Mikami; Nozomu Hanaoka; Shuji Ando; Naoko Honda; Kyle Taylor; Toshio Tsubota; Satoru Konnai; Haruo Watanabe; Makoto Ohnishi; Hiroki Kawabata

ABSTRACT Multilocus sequence typing of Borrelia garinii isolates from humans and comparison with rodent and tick isolates were performed. Fifty-nine isolates were divided into two phylogenetic groups, and an association was detected between clinical and rodent isolates, suggesting that, in Japan, human-pathogenic B. garinii comes from rodents via ticks.


Molecular Microbiology | 2009

LrhA positively controls the expression of the locus of enterocyte effacement genes in enterohemorrhagic Escherichia coli by differential regulation of their master regulators PchA and PchB

Naoko Honda; Sunao Iyoda; Shouji Yamamoto; Jun Terajima; Haruo Watanabe

Genes essential for eliciting pathogenicity of enterohemorrhagic Escherichia coli are located within the locus of enterocyte effacement (LEE). Expression of LEE genes is positively regulated by paralogues PchA, PchB and PchC, which are encoded by separate loci of the chromosome. To elucidate the underlying regulatory mechanism, we screened transposon mutants exhibiting reduced expression of pchA, transcription level of which is highest among the pch genes. Here, we report that the LysR‐homologue A (LrhA) positively regulated the transcription of pchA and pchB. A deletion in lrhA reduced the transcription levels of pchA and pchB to different degrees, and also reduced the expression of LEE‐coded type 3‐secreted protein, EspB. Expression of LrhA from a plasmid restored and markedly increased the transcription levels of pchA and pchB respectively, and highly induced EspB expression. Deletion analysis of the regulatory region showed that both promoter‐proximal (−195 to +88) and promoter‐distal (−418 to −392 for pchA and −391 to −375 for pchB) sequences were required for the LrhA‐mediated upregulation of pchA and pchB genes. Purified His6‐LrhA protein differentially bound to the regulatory regions of pchA/B, suggesting that direct regulation of pchA and pchB genes by LrhA in turn controls the expression of LEE genes.


Genetics | 2011

Diversity in Copy Number and Structure of a Silkworm Morphogenetic Gene as a Result of Domestication

Takashi Sakudoh; Takeharu Nakashima; Yoko Kuroki; Asao Fujiyama; Yuji Kohara; Naoko Honda; Hirofumi Fujimoto; Toru Shimada; Masao Nakagaki; Yutaka Banno; Kozo Tsuchida

The carotenoid-binding protein (CBP) of the domesticated silkworm, Bombyx mori, a major determinant of cocoon color, is likely to have been substantially influenced by domestication of this species. We analyzed the structure of the CBP gene in multiple strains of B. mori, in multiple individuals of the wild silkworm, B. mandarina (the putative wild ancestor of B. mori), and in a number of other lepidopterans. We found the CBP gene copy number in genomic DNA to vary widely among B. mori strains, ranging from 1 to 20. The copies of CBP are of several types, based on the presence of a retrotransposon or partial deletion of the coding sequence. In contrast to B. mori, B. mandarina was found to possess a single copy of CBP without the retrotransposon insertion, regardless of habitat. Several other lepidopterans were found to contain sequences homologous to CBP, revealing that this gene is evolutionarily conserved in the lepidopteran lineage. Thus, domestication can generate significant diversity of gene copy number and structure over a relatively short evolutionary time.


Journal of Computational Chemistry | 2012

A possible overestimation of the effect of acetylation on lysine residues in KQ mutant analysis.

Hirofumi Fujimoto; Mariko Higuchi; Manabu Koike; Hirotaka Ode; Miroslav Pinak; Juraj Kotulic Bunta; Toshiyuki Nemoto; Takashi Sakudoh; Naoko Honda; Hideaki Maekawa; Kimiaki Saito; Kozo Tsuchida

Acetylation of lysine residues, one of the most common protein post‐transcriptional modifications, is thought to regulate protein affinity with other proteins or nucleotides. Experimentally, the effects of acetylation have been studied using recombinant mutants in which lysine residues (K) are substituted with glutamine (Q) as a mimic of acetyl lysine (KQ mutant), or with arginine (R) as a mimic of nonacetylated lysine (KR mutant). These substitutions, however, have not been properly validated. The effects lysine acetylation on Ku, a multifunctional protein that has been primarily implicated in DNA repair and cell survival, are characterized herein using a series of computer simulations. The binding free energy was reduced in the KQ mutant, while the KR mutant had no effect, which is consistent with previous experimental results. Unexpectedly, the binding energy between Ku and DNA was maintained at almost the same level as in the wild type protein despite full acetylation of the lysine residues. These results suggest that the effects of acetylation may be overestimated when the KQ mutant is used as a mimic of the acetylated protein.


Infection and Immunity | 2011

Coordinate Control of the Locus of Enterocyte Effacement and Enterohemolysin Genes by Multiple Common Virulence Regulators in Enterohemorrhagic Escherichia coli

Sunao Iyoda; Naoko Honda; Takehito Saitoh; Ken Shimuta; Jun Terajima; Haruo Watanabe; Makoto Ohnishi

ABSTRACT The locus of enterocyte effacement (LEE) pathogenicity island is required for the intimate adhesion of enterohemorrhagic Escherichia coli (EHEC) to the intestinal epithelial cells. GrlR and GrlA are LEE-encoded negative and positive regulators, respectively. The interaction of these two regulators is important for controlling the transcription of LEE genes through Ler, a LEE-encoded central activator for the LEE. The GrlR-GrlA regulatory system controls not only LEE but also the expression of the flagellar and enterohemolysin (Ehx) genes in EHEC. Since Ehx levels were markedly induced in a grlR mutant but not in a grlR grlA double mutant and significantly increased by overexpression of GrlA in a ler mutant, GrlA is responsible for this regulation (T. Saitoh et al., J. Bacteriol. 190:4822-4830, 2008). In this study, additional investigations of the regulation of ehx gene expression determined that Ler also acts as an activator for Ehx expression without requiring GrlA function. We recently reported that the LysR-type regulator LrhA positively controls LEE expression (N. Honda et al., Mol. Microbiol. 74:1393-1411, 2009). The hemolytic activity of the lrhA mutant strain of EHEC was lower than that of the wild-type strain, and LrhA markedly induced ehx transcription in an E. coli K-12 strain, suggesting that LrhA also activates the transcription of ehx without GrlA and Ler. Gel mobility shift assays demonstrated that Ler and LrhA directly bind to the regulatory region of ehxC. Together, these results indicate that transcription of ehx is positively regulated by Ler, GrlA, and LrhA, which all act as positive regulators for LEE expression.


Journal of Lipid Research | 2013

Lipid transfer particle from the silkworm, Bombyx mori, is a novel member of the apoB/large lipid transfer protein family.

Hiroshi Yokoyama; Takeru Yokoyama; Masashi Yuasa; Hirofumi Fujimoto; Takashi Sakudoh; Naoko Honda; Hajime Fugo; Kozo Tsuchida

Lipid transfer particle (LTP) is a high-molecular-weight, very high-density lipoprotein known to catalyze the transfer of lipids between a variety of lipoproteins, including both insects and vertebrates. Studying the biosynthesis and regulation pathways of LTP in detail has not been possible due to a lack of information regarding the apoproteins. Here, we sequenced the cDNA and deduced amino acid sequences for three apoproteins of LTP from the silkworm (Bombyx mori). The three subunit proteins of the LTP are coded by two genes, apoLTP-II/I and apoLTP-III. ApoLTP-I and apoLTP-II are predicted to be generated by posttranslational cleavage of the precursor protein, apoLTP-II/I. Clusters of amphipathic secondary structure within apoLTP-II/I are similar to Homo sapiens apolipoprotein B (apoB) and insect lipophorins. The apoLTP-II/I gene is a novel member of the apoB/large lipid transfer protein gene family. ApoLTP-III has a putative conserved juvenile hormone-binding protein superfamily domain. Expression of apoLTP-II/I and apoLTP-III genes was synchronized and both genes were primarily expressed in the fat body at the stage corresponding to increased lipid transport needs. We are now in a position to study in detail the physiological role of LTP and its biosynthesis and assembly.


PLOS ONE | 2017

Enzymatic activity necessary to restore the lethality due to Escherichia coli RNase E deficiency is distributed among bacteria lacking RNase E homologues

Masaru Tamura; Daisuke Kageyama; Naoko Honda; Hirofumi Fujimoto; Atsushi Kato

Escherichia coli RNase E (Eco-RNase E), encoded by rne (Eco-rne), is considered the global RNA decay initiator. Although Eco-RNase E is an essential gene product in E. coli, some bacterial species, such as Bacillus subtilis, do not possess Eco-RNase E sequence homologues. B. subtilis instead possesses RNase J1/J2 (Bsu-RNase J1/J2) and RNase Y (Bsu-RNase Y) to execute RNA decay. Here we found that E. coli lacking the Eco-rne gene (Δrne E. coli) was viable conditional on M9 minimal media by introducing Bsu-RNase J1/J2 or Bsu-RNase Y. We also cloned an extremely short Eco-RNase E homologue (Wpi-RNase E) and a canonical sized Bsu-RNase J1/J2 homologue (Wpi-RNase J) from Wolbachia pipientis, an α-proteobacterial endosymbiont of arthropods. We found that Wpi-RNase J restored the colony-forming ability (CFA) of Δrne E. coli, whereas Wpi-RNase E did not. Unexpectedly, Wpi-RNase E restored defective CFA due to lack of Eco-RNase G, a paralogue of Eco-RNase E. Our results indicate that bacterial species that lack Eco-RNase E homologues or bacterial species that possess Eco-RNase E homologues which lack Eco-RNase E-like activities have a modest Eco-RNase E-like function using RNase J and/or RNase Y. These results suggest that Eco-RNase E-like activities might distribute among a wide array of bacteria and that functions of RNases may have changed dynamically during evolutionary divergence of bacterial lineages.


Archives of Microbiology | 2016

PpsA-mediated alternative pathway to complement RNase E essentiality in Escherichia coli.

Masaru Tamura; Naoko Honda; Hirofumi Fujimoto; Stanley N. Cohen; Atsushi Kato

Escherichia coli cells require RNase E, encoded by the essential gene rne, to propagate. The growth properties on different carbon sources of E. coli cells undergoing suppression of RNase E production suggested that reduction in RNase E is associated with decreased expression of phosphoenolpyruvate synthetase (PpsA), which converts pyruvate to phosphoenolpyruvate during gluconeogenesis. Western blotting and genetic complementation confirmed the role of RNase E in PpsA expression. Adventitious ppsA overexpression from a multicopy plasmid was sufficient to restore colony formation of ∆rneE. coli on minimal media containing glycerol or succinate as the sole carbon source. Complementation of ∆rne by ppsA overproduction was observed during growth on solid media but was only partial, and bacteria showed slowed cell division and grew as filamentous chains. We found that restoration of colony-forming ability by ppsA complementation occurred independent of the presence of endogenous RNase G or second-site suppressors of RNase E essentiality. Our investigations demonstrate the role of phosphoryl transfer catalyzable by PpsA as a determinant of RNase E essentiality in E. coli.


Protein Expression and Purification | 2015

Purification and functional characterization of diadenosine 5′,5‴-P1,P4-tetraphosphate phosphorylases from Mycobacterium smegmatis and Mycobacterium avium

Naoko Honda; Hyun Wha Kim; Emiko Rimbara; Atsushi Kato; Shigetarou Mori


The Molecular Biology Society of Japan | 2016

Enzymatic activity necessary to complement Escherichia coli RNase E deficiency is widely distributed among bacterial species lacking RNase E homologues

Masaru Tamura; Daisuke Kageyama; Naoko Honda; Hirofumi Fujimoto; Atsushi Kato

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Hirofumi Fujimoto

National Institutes of Health

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Atsushi Kato

National Institutes of Health

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Kozo Tsuchida

National Institutes of Health

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Takashi Sakudoh

National Institutes of Health

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Daisuke Kageyama

National Agriculture and Food Research Organization

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Hideaki Maekawa

National Institutes of Health

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Jun Terajima

National Institutes of Health

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Makoto Ohnishi

National Institutes of Health

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