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Dive into the research topics where Natalia Bellostas is active.

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Featured researches published by Natalia Bellostas.


Journal of Natural Products | 2008

Fe2+-catalyzed formation of nitriles and thionamides from intact glucosinolates.

Natalia Bellostas; Anne Dorthe Sørensen; Jens Christian Sørensen; Hilmer Sørensen

The ratio of isothiocyanates to nitriles formed upon the hydrolysis of glucosinolates is a key factor determining the physiological effect of glucosinolate-containing plants and materials. A micellar electrokinetic capillary chromatography (MECC) method was used to study the nonenzymatic Fe2+-catalyzed transformation of glucosinolates. At room temperature, pH 5, and in the presence of only 2 molar excess of Fe2+ all glucosinolate was degraded in 24 h. At all molar excess Fe2+ tested, nitriles were the major compounds formed. Thionamides were also formed from glucosinolates that contained a side chain hydroxylated at C-2; in this case, trace amounts of oxazolidine-2-thione were also detected. The presence of Fe3+ had no effect. The nonenzymatic Fe2+-catalyzed transformation of glucosinolates involves the binding of Fe2+ to the glucosinolate to form a complex.


Advances in Botanical Research | 2007

Genetic Variation and Metabolism of Glucosinolates

Natalia Bellostas; Anne Dorthe Sørensen; Jens Christian Sørensen; Hilmer Sørensen

Progress in the production and utilisation of oilseed rape (Brassica napus L. and B. rapa L.) and their derived products in the past years has resulted in the production of high‐yielding double low varieties with low levels of erucic acid and glucosinolates. In order to meet this objective, extensive knowledge on the occurrence, structure and properties of glucosinolates and myrosinase isoenzymes (thioglucohydrolase; EC 3.2.1.147) co‐occurring with glucosinolates is essential. The present chapter focusses on these subjects including heredity, genetic variation and metabolism of glucosinolates. A number of stages in the glucosinolate biosynthetic pathway are well known, but significant elements still remain to be further explored and elucidated. In recent years, most research has been confined on gene technology using Arabidopsis thaliana as a model and hence considering only the glucosinolates present in this particular plant. To date, more than 140 structurally different glucosinolates have been described of which ∼30 glucosinolates occur in Brassica species. Only relatively few glucosinolates are produced in individual plant genera and/or families. Knowledge on the genetic‐ and metabolic‐based regulation of glucosinolate accumulation in different plants is therefore of great relevance in order to explain the reasons for the chemotaxonomically limited occurrence of structurally different glucosinolates. The metabolic regulation and thus the biological consequences of those changes are however unknown. Similarly, the reasons for the high selectivity of the multi‐enzyme complexes towards the precursor amino acids in the biosynthesis of glucosinolates, as well as the specificity of the various enzymes needed for the modification of glucosinolate side chains and the substitution/esterification of the thioglucose residue of the molecules are yet to be clarified. The glucosinolate catabolism, which comprises the transformation of glucosinolates into various bioactive products, is another important area where limited information is available on the oligomeric myrosinase isoenzymes and associated compounds. This is especially relevant regarding the differences in the catalytic properties of the individual isoenzymes as well as the opportunities to modify these properties. New analytical methods allow for the synchronous detection of substrate and products in myrosinase‐catalysed reactions, which broadens the perspectives for progress in this field.


Scientia Horticulturae | 2007

Glucosinolate profiling of seeds and sprouts of B. oleracea varieties used for food

Natalia Bellostas; Piotr Kachlicki; Jens Christian Sørensen; Hilmer Sørensen


Journal of the Science of Food and Agriculture | 2007

Profiling glucosinolates in vegetative and reproductive tissues of four Brassica species of the U-triangle for their biofumigation potential

Natalia Bellostas; Jens Christian Sørensen; Hilmer Sørensen


Journal of Biochemical and Biophysical Methods | 2008

A fast and gentle method for the isolation of myrosinase complexes from Brassicaceous seeds

Natalia Bellostas; Iben Lykke Petersen; Jens Christian Sørensen; Hilmer Sørensen


Journal of Chromatography A | 2006

Micellar electrokinetic capillary chromatography—Synchronous monitoring of substrate and products in the myrosinase catalysed hydrolysis of glucosinolates

Natalia Bellostas; Jens Christian Sørensen; Hilmer Sørensen


African Journal of Agricultural Research | 2010

Glucosinolates in leaves of Moringa species grown and disseminated in Niger

Natalia Bellostas; Jens Christian Sørensen; Albert Nikiema; Hilmer Sørensen; Sanjeet Kumar


Archive | 2007

Processing-bioprocessing of oilseed rape in bioenergy production and value added utilization of remaining seed components

Christian Bagger; Natalia Bellostas; Søren Krogh Jensen; Jens Christian Sørensen; Hilmer Sørensen; Susanne Sørensen


Journal of Molecular Catalysis B-enzymatic | 2009

Type and concentration of redox reagents influencing nitrile formation upon myrosinase (Brassica carinata)-catalyzed hydrolysis of glucosibarin

Natalia Bellostas; Anne Dorthe Sørensen; Jens Christian Sørensen; Hilmer Sørensen


Archive | 2007

Nutritional value of cruciferous oilseed crops in relation to profile of accumulated biomolecules with especial regard to glucosinolates transformation products

Natalia Bellostas; Charlotte Bjergegaard; Søren Krogh Jensen; Hilmer Sørensen; Jens Christian Sørensen; Susanne Sørensen

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Piotr Kachlicki

Polish Academy of Sciences

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Sanjeet Kumar

International Crops Research Institute for the Semi-Arid Tropics

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