Nathalya Botelho Brito

Effects of andiroba (Carapa guianensis) oil on hepatic function of rats subjected to liver normothermic ischemia and reperfusion.

OBJECTIVE To evaluate the effects of the Andiroba (carapa guianensis) oil on liver function in rats subjected to normothermic ischemia / reperfusion injury. METHODS we divided 12 Wistar rats into two groups: saline (n = 6) and Andiroba (n = 6). The Andiroba group was treated with Andiroba oil (0.63 ml/kg orally) for seven days before surgery. Ischemia was induced by occlusion of the blood supply to the lateral and median lobes of the liver, using vascular clips, in both groups, for 45min, followed by reperfusion for 60 minutes later. We analyzed dosages of AST, ALT, Gamma-GT, and liver biodistribution of 99mTc phytate. RESULTS There was no significant difference in the percentage of radioactivity / gram of tissue (%ATI/g) in the right lobe of the saline group (17.53 ± 2.78) compared with the Andiroba group (18.04 ± 3.52) p = 0.461, the same occurring in the%ATI/g of the left lobe of the liver when the two groups were compared (p = 0.083). In the saline group, the%ATI/g was significantly higher in the non-ischemic right hepatic lobe (17.53 ± 2.78) when compared with the left lobe (5.04 ± 0.82) that suffered ischemia / reperfusion (p = 0.002). Significant differences also occurred when comparing the right (18.04 ± 3.52) and left (7.11 ± 1.86) lobes of the animals of the Andiroba group (p = 0.004). There was no significant difference in dosages of AST, ALT and Gamma- GT when comparing the two groups (p > 0.05). CONCLUSION Andiroba oil did not contribute to the protection of liver function in a rat model of liver injury induced by normothermic ischemia and reperfusion.

Immediate graft histological assay, post pig's liver transplantation

PURPOSE To describe the vascular and tissue histopathological changes in seven sequential experimental liver transplantations in pigs. METHODS Fourteen female pigs, Sus domesticus species, with body mass between 5 and 8 kg were utilized. After the end of all anastomoses of the graft implantation in the receptor, the animal was monitored for 30 minutes, and at its end one of the biopsies was collected for histological analysis. The histological criteria utilized were: lytic hepatocyte necrosis, density of septal and portal inflammatory infiltrated, sinusoidal congestion and hemorrhage. The analysis was performed separately for the portal region in zone 1, 2 and 3. RESULTS Among the structural changes undergone by the graft, those with greater frequency and intensity were vascular congestion and steatosis, which stood out in transplantations 5, 6 and 7. CONCLUSIONS The technique demonstrated vascular alterations represented by vasocongestion, edema and minimum inflammatory reaction. In relation to the parenchyma, was observed macrovacuolar pan-acinar steatosis, focal lytic and occasional hemorrhages, beyond the accumulation of hemosiderin in Kuppfers cells.