Necdet Adanir

Bleaching and Desensitizer Application Effects on Shear Bond Strengths of Orthodontic Brackets

OBJECTIVE To evaluate the effects of bleaching and desensitizer application on shear bond strengths of orthodontic brackets. MATERIALS AND METHODS Forty-eight extracted human premolar teeth were randomly assigned to 4 groups of 12 each. The first group of teeth was bleached with a 35% hydrogen peroxide office bleaching agent. The second group was bleached the same as the first group and UltraEZ desensitizer was applied. No bleaching procedures were applied on the third and fourth groups. UltraEZ desensitizer alone was applied to teeth in the third group. The fourth group served as control. Orthodontic brackets were bonded with a LC (light cure) composite resin and cured with a halogen light. After bonding, the shear bond strengths of the brackets were tested with a Universal testing machine. RESULTS The results showed that bleaching, bleaching plus desensitizer, and desensitizer procedures significantly reduced the bonding strengths of the orthodontic brackets (P < .05, P < .001, and P < .01, respectively). No statistically significant difference was found between bleaching, bleaching plus desensitizer, and desensitizer groups (P > .05). CONCLUSIONS Because bleaching and desensitizer application significantly affected shear bond strengths of orthodontic brackets on human enamel, they should be delayed until the completion of orthodontic treatment.

Effects of adhesion promoters on the shear bond strengths of orthodontic brackets to fluorosed enamel

The aims of this in vitro study were to evaluate the effect of enamel fluorosis on the shear bond strength (SBS) of orthodontic brackets and to determine whether adhesion promoter, Enhance LC, increases the bond strength of brackets to fluorosed enamel. Forty-five (30 fluorosed and 15 non-fluorosed) non-carious fresh human premolar teeth, extracted for orthodontic reasons and without any caries or visible defects, were used in this study. The fluorosed teeth were selected according to the modified Thylstrup and Fejerskov index, which is based on the clinical changes in fluorosed teeth. In groups 1 (fluorosed teeth) and 3 (control), the brackets were bonded with Light Bond composite resin and cured with a halogen light. In group 2, Enhance LC was applied to fluorosed enamel before bonding. After bonding, the SBS of the brackets was tested with a universal testing machine. One-way analysis of variance and Tukey multiple comparison tests were used to compare the SBS of the groups. Any adhesive remaining after debonding was assessed and scored according to the modified adhesive remnant index. The results showed that while fluorosis significantly reduced the bond strengths of the orthodontic brackets (mean 13.94 +/- 3.24 MPa; P < 0.01), Enhance LC significantly increased bond strength on fluorosed enamel (mean 18.22 +/- 5.97 Mpa; P < 0.05). Groups 1 and 3 had greater bond failures at the composite-bracket interface, whereas group 2 showed bond failure primarily at the enamel-composite interface.

In vitro evaluation of shear bond strengths of colour change adhesives.

The aims of this study were to test whether the shear bond strengths (SBS) of three commercially available colour change adhesives (CCAs), Transbond Plus Color Change Adhesive, Grēngloo, and Blūgloo, are different and to compare their bond strengths with a traditional light cure adhesive, Light Bond. Forty-eight human permanent premolar teeth extracted for orthodontic reasons and without any caries or visible defects were used in this study. The brackets were bonded with Light Bond (group I), Grēngloo (group II), Blūgloo (group III), and Transbond Plus CCA (group IV). After bonding, the SBS of the brackets were tested with a Universal testing machine. Analysis of variance indicated a significant difference between groups I and IV (P < 0.001). No significant difference was found between groups II, III, and IV (P > 0.05). Adhesive remnant index (ARI) scores for all groups were not significantly different (P > 0.05). Significant difference existed between the SBS of Transbond Plus CCA and Light Bond. Although Transbond Plus CCA yielded the lowest SBS values, no statistically significant difference was found between bond strength values of the three commercially available CCAs. All three CCAs can be safely used in orthodontic practice since they yielded acceptable bond strengths. A higher incidence of ARI scores 4 and 5 revealed that bond failures in all test groups were mainly at the adhesive interface.

Effects of Potassium Nitrate and Oxalate Desensitizer Agents on Shear Bond Strengths of Orthodontic Brackets

OBJECTIVE To evaluate the effects of potassium nitrate and oxalate desensitizer agents on shear bond strengths of orthodontic brackets. MATERIALS AND METHODS Forty-five extracted human premolar teeth were randomly assigned to three groups of 15 each. UltraEZ potassium nitrate desensitizer was applied on teeth in the first group, while BisBlock oxalate desensitizer was applied on teeth in the second group. The third group served as a control. Orthodontic brackets were bonded with a light cure composite resin and cured with a halogen light. After bonding, the shear bond strength of the brackets was tested with a universal testing machine. RESULTS The highest shear bond strengths were measured in Group III. The shear bond strength in Groups I and II was significantly lower than in Group III (P < .001). Significant difference was also found between Group I and Group II (P < .01). CONCLUSIONS Orthodontic brackets bonded to enamel treated with potassium nitrate and oxalate desensitizers showed significantly lower bond strengths than did brackets bonded to untreated enamel.

Comparison of cytotoxicity of various concentrations origanum extract solution with 2% chlorhexidine gluconate and 5.25% sodium hypochlorite

Objective: The purpose of this in vitro study was to compare the cytotoxicity of 0.5-4.5 origanum extract solution (OES), 2% chlorhexidine gluconate (CHX) and 5.25% sodium hypochlorite (NaOCl) with WST-1 test on human periodontal ligament (hPDL) fibroblasts. Materials and Methods: About 0.5-4.5% OES, 2% CHX and 5.25% NaOCl solutions cytotoxicity was evaluated with cell culture test using PDL fibroblasts. Viability of hPDL cells was evaluated with WST-1 (Cell Proliferation Reagent WST-1 Roche) test at 1, 24 and 72 nd h. hPDL cells were plated at 20 × 10 3 cells per well in 96-well plates. Absorbance values were read in optical density 480 nm by ELISA plate reader spectrophotometer. The statistical differences between various groups were evaluated using one-way ANOVA, post-hoc Duncan′s Multiple Range test using SAS software. Statistically, a significant difference was considered at P < 0.001. Results: According to the 1-h cytotoxicity results, 0.5% OES showed the least cytotoxic effect in test groups. There were not found any statistical significance between 1% OES and 2% CHX. About 5.25% NaOCl showed more cytotoxic effect than 1% OES and 2% CHX. In 24 and 72 h, different concentrations of OES, 5.25% NaOCl, 2% CHX solutions showed similar cytotoxic effect. Conclusions: Based on these results, 1% OES and 2% CHX showed similar results and less cytotoxic effect than 5.25% NaOCl. It could be considered as a favorable solution concentration when OES was used as root canal irrigation solution.

Microleakage under orthodontic brackets bonded with different adhesive systems.

Objective: This in vitro study aimed to compare the microleakage of orthodontic brackets between enamel-adhesive and adhesive-bracket interfaces at the occlusal and gingival margins bonded with different adhesive systems. Materials and Methods: A total of 144 human maxillary premolar teeth extracted for orthodontic reasons was randomly divided into four groups. Each group was then further divided into three sub-groups. Three total-etching bonding systems (Transbond XT, Greengloo and Kurasper F), three one-step self-etching bonding systems (Transbond Plus SEP, Bond Force and Clearfil S3), three two-step self-etching bonding systems (Clearfil SE Bond, Clearfil Protectbond and Clearfil Liner Bond), and three self-adhesive resin cements (Maxcem Elite, Relyx U 100 and Clearfil SA Cement) were used to bond the brackets to the teeth. After bonding, all teeth were sealed with nail varnish and stained with 0.5% basic fuchsine for 24 h. All samples were sectioned and examined under a stereomicroscope to score for microleakage at the adhesive-enamel and adhesive-bracket interfaces from both occlusal and gingival margins. Statistical Analysis Used: Statistical analyses were performed with Kruskal-Wallis and Wilcoxon signed-rank tests. Results: The results indicate no statistically significant differences between the microleakage scores of the adhesives; microleakage was detected in all groups. Comparison of the average values of the microleakage scores in the enamel-adhesive and adhesive-bracket interfaces indicated statistically significant differences (P < 0.05). The amount of the microleakage was higher at the enamel-adhesive interface than at the bracket-adhesive interface. Conclusions: All of the brackets exhibited some amount of microleakage. This result means that microleakage does not depend on the type of adhesive used.

Fracture Resistance of Roots Restored with Four Different Fiber-Reinforced Composite Posts.

Objective: The aim of this study was to compare the resistance to vertical root fracture of root-filled teeth restored with four different fiber-reinforced composite (FRC) post systems and two types of dual-cured resin luting agents. Materials and Methods: Ninety extracted human maxillary central incisors were selected and decoronated to obtain a standardized root length of 14 mm. After root canal obturation, post spaces were prepared to a depth of 10 mm with a No. 3 post drill. The specimens (n = 80) were divided into two groups (n = 40) according to the resin luting agents used: group 1, Variolink II + ExciTE DSC; group 2, RelyX Unicem. These groups were subdivided into four subgroups (n = 10) and restored with one of the following post systems: (a) DT Light, (b) DT Light SL, (c) FRC Postec and (d) Everstick, while the remaining 10 teeth served as controls. The roots were subjected to axial compressive loading using a 2.2-mm-diameter metal sphere in a universal testing machine (0.5 mm/min). A factorial experiment with a single control group (analysis of variance) was used to test the resistance of the specimens. Results: Groups 2a (DT Light + RelyX Unicem; 398.5 N) and 1b (DT Light SL + Variolink II + ExciTE DSC; 431.1 N) had significantly higher resistance to fracture than the control group (334.1 N; p < 0.05). DT Light SL and FRC Postec Plus were more resistant to fracture when Variolink II was used as the luting cement. DT Light and Everstick had higher fracture resistance when they were luted with RelyX Unicem (p < 0.05). Conclusion: The results of this study indicate that the use of quartz fiber posts (DT Light and DT Light SL) with an adhesive luting cement in root-filled teeth may reinforce the root to some extent.

Antibacterial and smear layer removal capability of oregano extract solution.

Objective: The aim of this in vitro study was to evaluate the antimicrobial effect of oregano extract solution (OES) against Enterococcus faecalis within root canals and dentin tubules, and its effect on smear layer. Materials and Methods: A total of 180 human maxillary central incisors was selected. After removal of coronal part of the teeth, root canals were prepared using ProTaper rotary files (Dentsply, Tulsa Endodontics, OK, USA) to #F3 with the crown-down manner. The roots were randomly assigned to 15 groups (n = 12 for each). In the first seven groups, the antimicrobial effects of the test groups were evaluated. Suspensions of E. faecalis cultures were adjusted to 1.0 McFarland (1 × 10 8 colony-forming unit [CFU]/ml), and sterilized teeth were placed in Eppendorf tubes and kept at 37°C for 4 weeks. Samples were then taken from the root canals before irrigation using three sterile paper points. Dentin samples were taken from root canals with ProTaper #F4 and #F5 series rotary instruments after irrigation. The aliquots of samples were placed into the brain heart infusion and incubated at 37°C for 48 h and then the CFUs were counted. In the other eight groups, the efficacy of the irrigation solutions on removing the smear layer was evaluated using scanning electron microscope (Leo 440, Oxford Microscopy Ltd., Cambridge, England) analysis. Statistical evaluation of the microbiological data was performed using the Kruskall-Wallis and Mann-Witney U-test (P < 0.05). Results: There was a statistically difference between the groups (P < 0.05). Chlorhexidine gluconate (CHX), 5% and 2% OES wasn′t found to be statistically significant regarding their antibacterial activities against E. faecalis (P > 0.05). 1% OES and NaOCl showed similar antimicrobial effect (P > 0.05), and 1% OES and NaOCl were better than ethylenediaminetetraacetic acid (EDTA) and saline (P < 0.05) but not as successful as CHX. According to the results obtained from dentin, CHX is the most effective solution within dentinal tubules. Different concentrations of OES were not achieved smear layer removal alone but OES in conjunction with 17% EDTA was the final irrigating solution achieved the smear layer removal without dentin erosion. Conclusions: Within the limitations of this study, OES appears to be a possible alternative to NaOCl as a root canal irrigant on the eradication of E. faecalis and removal of smear layer.