Nery Sofiyanti

Immunomodulatory Effectiveness of Aqueous Obat Pahit Extract of Lingga Malay Ethnic on White Rats (Rattus novergicus)

Obat pahit has been generally known and believed by Lingga Malay society as anti-aging agent. However, the study of Obat pahit is not scientifically proven. This research was aimed to prove immunomodulatory ability of Obat pahit potion from Lingga, Riau Archipelago. This study used white rats as an animal modelling, and Staphylococcus aureus as bacteria tester. The rats had been treated with aqueous Obat pahit extract from three TMPs on dose scales of 0.09, 0.18 and 0.27 mL/200g of body weight through oral administration for 7 days. Furthermore, on the 8 th days, the experiment animals were injected by the preparation of bacteria tester through intraperitoneal administration in the amount of 0.5 mL/200 gram of body weigth and subsequently incubated for 1 hour after the injection. There were 2 observed parameters on this study, i.e efectivity and capacity of phagocytosis by leukocytes. The observation of leukocytes-phagocytocis activity was carried out by making a smear preparat samples of peritoneum fluid from rats. After the observation under microscope on a magnification of 100 times. The result was obtained the Obat pahit from Kalan PMT swere more effective on dose 2, while from SP4 and Linau TMPs were much more effective on dose 1. It is therefore, using these data of the results, the advanced doses scale of this Obat pahit would not be necessary. Obat pahit potion from Malay Lingga Malay Ethnic could become raw materials of immunomodulatory herbal medicine based on traditional knowledge. It also potentially as a standardized herbal.

Short Communication: Phylogenetic analysis of mango (Mangifera) in Northern Sumatra based on gene sequences of cpDNA trnL-F intergenic spacer

Harahap SP, Fitmawati, Sofiyanti N. 2017. Short Communication: Phylogenetic analysis of mango (Mangifera) in Northern Sumatra based on gene sequences of cpDNA trnL-F intergenic spacer. Biodiversitas 18: 715-719. Northern Sumatra is an area with geographical variation. The environmental factors are affected on character plasticity such as found in Anacardiaceae family especially Mangifera genus. The character plasticity of Mangifera members raises a problem in determining clear boundaries between species based on morphological character. Therefore, a molecular approach is necessary to provide a specific character among Mangifera species. This study aimed to reconstruct the phylogeny among Mangifera members in Northern Sumatra based on the sequences of trnLF intergenic spacer. All of the sequences were aligned by using Clustal W and Cladogram was reconstructed by using PAUP by Maximum Parsimony (MP) and Neighbour-Joining (NJ) methods. The MP cladogram produced two in groups i.e., clade I consist of M. odorata1, M. odorata2, M. laurina1, M. laurina2, M. indica, M. zeylanica, M. quadrifida and Mangifera sp. and clade II consisted of M. foetida1 and M. foetida2. Based on the NJ method, it was obtained M. laurina2 which had the highest genetic distance among other members of Mangifera. The trnL-F intergenic spacer with a low sequences variation indicated that this region was highly conserved and had a low evolution rate in Mangifera.