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Dive into the research topics where Nghia Nguyen Do Trong is active.

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Featured researches published by Nghia Nguyen Do Trong.


Innovative Food Science and Emerging Technologies | 2014

Optical properties-microstructure-texture relationships of dried apple slices: spatially resolved diffuse reflectance spectroscopy as a novel technique for analysis and process control

Nghia Nguyen Do Trong; Anna Rizzolo; Els Herremans; M. Vanoli; Giovanna Cortellino; Chyngyz Erkinbaev; Mizuki Tsuta; Lorenzo Spinelli; Davide Contini; Alessandro Torricelli; Pieter Verboven; Josse De Baerdemaeker; Bart Nicolai; Wouter Saeys

The potential of spatially resolved diffuse reflectance spectroscopy in the 500-1000 nm range by means of a fiber-optics probe was investigated for acquiring scattering and absorption properties of air dried apple rings subjected to different pre-treatment conditions: without osmo-dehydration (TQ) and with osmo-dehydration for 1 (OSMO1) and 3 hours (OSMO2). The fresh apple rings were produced from ‘Golden Delicious’ apples at harvest (H) and 5 month storage at 2 conditions: controlled atmosphere (CA) and normal atmosphere (NA). Microstructure properties of the dried apple rings were also obtained from X-ray micro-CT measurements. The TQ samples were found to have significantly higher scattering properties, thicker tissue, smaller pore sizes, were less crispy, and required higher snapping work or rupture energy than the OSMO1 and OSMO2 samples. On the other hand, no significant differences were observed between the scattering properties, microstructure, and textural quality of the OSMO1 and OSMO2 apple rings. From these results, it was concluded that there is a clear process-microstructure-quality relation in osmo-air-dried apples which can be measured non-destructively with spatially resolved diffuse reflectance spectroscopy. AC C EP TE D M AN U SC R IP T ACCEPTED MANUSCRIPT 2 Therefore, this study confirmed the potential of spatially resolved diffuse reflectance spectroscopy for non-destructive quality assessment of air-dried apple slices, which provides perspectives for drying process optimization.


Optics Express | 2013

Metamodeling approach for efficient estimation of optical properties of turbid media from spatially resolved diffuse reflectance measurements

Rodrigo Watté; Nghia Nguyen Do Trong; Ben Aernouts; Chyngyz Erkinbaev; Josse De Baerdemaeker; Bart Nicolai; Wouter Saeys

A metamodeling approach is introduced and applied to efficiently estimate the bulk optical properties of turbid media from spatially resolved spectroscopy (SRS) measurements. The model has been trained on a set of liquid phantoms covering a wide range of optical properties representative for food and agricultural products and was successfully validated in forward and inverse mode on phantoms not used for training the model. With relative prediction errors of 10% for the estimated bulk optical properties the potential of this metamodeling approach for the estimation of the optical properties of turbid media from spatially resolved spectroscopy measurements has been demonstrated.


Journal of Biophotonics | 2016

Light distribution and thermal effects in the rat brain under optogenetic stimulation

Barbara Gysbrechts; Ling Wang; Nghia Nguyen Do Trong; Henrique Cabral; Zaneta Navratilova; Francesco P. Battaglia; Wouter Saeys; Carmen Bartic

Optical brain stimulation gained a lot of attention in neuroscience due to its superior cell-type specificity. In the design of illumination strategies, predicting the light propagation in a specific tissue is essential and requires knowledge of the optical properties of that tissue. We present the estimated absorption and reduced scattering in rodent brain tissue using non-destructive contact spatially resolved spectroscopy (cSRS). The obtained absorption and scattering in the cortex, hippocampus and striatum are similar, but lower than in the thalamus, leading to a less deep but broader light penetration profile in the thalamus. Next, the light distribution was investigated for different stimulation protocols relevant for fiber-optic based optogenetic experiments, using Monte Carlo simulation. A protocol specific analysis is proposed to evaluate the potential of thermally induced side effects.


Food Microstructures#R##N#Microscopy, Measurement and Modelling | 2013

Optical coherence tomography (OCT), space-resolved reflectance spectroscopy (SRS) and time-resolved reflectance spectroscopy (TRS): principles and applications to food microstructures

Alessandro Torricelli; Lorenzo Spinelli; M. Vanoli; Michael Leitner; Alexandra Nemeth; Nghia Nguyen Do Trong; Bart Nicolai; Wouter Saeys

Abstract: This chapter presents the recent developments in advanced optical methods for exploring food microstructure. The chapter first discusses the basics of light propagation in food and the main limitations of classical approaches (e.g. continuous wave near infrared (NIR), colorimetry) for the measurement of the optical properties of food. It then describes the physical principles, the technological solutions and the advantages of optical coherence tomography, and of space- and time-resolved reflectance spectroscopy. The chapter includes examples of applications and an overview of future prospects.


IFAC Proceedings Volumes | 2013

Estimation of Pear Ripeness by Hyperspectral Laser Scatter Imaging

Hiromichi Itoh; Josse De Baerdemaeker; Nghia Nguyen Do Trong; Ling Wang; Wouter Saeys

Abstract The purpose of this research is to analyse relationships between pear ripeness and subsurface scattering, Tested sample was pear ( Pyrus communis L., “Doyenne du Comice”). Soluble solids content and firmness were measured as indices of pear ripeness. Dispersed light in the range from 450 nm to 1000 nm in 5 nm intervals was irradiated at 1 mm diameter spot on pear surface. Light scattering around the spot was captured by a CCD camera. Area and mean diameter of the scattering region in each wavelength image were measured by image-processing. Relationships between the ripeness and the area or mean diameter were analysed by PLS (partial least squares) and PCR (principal component regression) methods. The results showed that the correlation coefficient between the measured and estimated ripeness was 0.653 for soluble solids content and 0.609 for firmness when the scattering images were measured on peel.


Biotechnology Progress | 2011

The potential of spatially resolved spectroscopy for monitoring angiogenesis in the chorioallantoic membrane.

Eva Verhoelst; Flip Bamelis; Bart De Ketelaere; Nghia Nguyen Do Trong; Josse De Baerdemaeker; Wouter Saeys; Mizuki Tsuta; Eddy Decuypere

Over the last decade, the poultry sector has sought to develop ways to monitor chicken embryonic development as to optimize the incubation conditions. One of the parameters of development which may change under different incubation conditions is the angiogenesis in the chorioallantoic membrane (CAM). To be able to quantify these changes in the angiogenesis and detect long‐term effects on health, a non‐destructive technique is necessary. In this article, the first steps toward such a non‐destructive technique are successfully taken. A spatially resolved spectroscopy set‐up is built and tested for its potential to measure changes in angiogenesis with incubation time, and differences between a normal and hypercapnic incubation. In this first study, reflectance measurements are performed directly on the CAM as the eggshell considerably complicates the analysis. This issue should be addressed in future research to come to a really non‐destructive technique. An experiment was conducted in which one group was incubated under normal conditions, and another under early prenatal hypercapnic conditions (i.e., increased CO2 concentrations). The angiogenesis in the CAM was measured at embryonic day (ED) 10, 13, and 16. The measurements showed a clear blood spectrum with an increasing amount of blood in time, and significant differences in the reflectance as function of the source‐detector distances. However, no significant differences between the hypercapnia and the control group could be detected.


2011 Louisville, Kentucky, August 7 - August 10, 2011 | 2011

Spatially Resolved Spectroscopy for Non-destructive Quality Inspection of Foods

Nghia Nguyen Do Trong; Rodrigo Watté; Ben Aernouts; Els Herremans; Eva Verhoelst; Mizuki Tsuta; Pieter Verboven Asabe Member; Josse De Baerdemaeker Asabe Member; Bart M Nicolaï Asabe Member; Wouter Saeys Asabe Member

Food quality is critically determined by its microstructure and composition. These properties could be quantified non-invasively by means of optical properties (absorption and reduced scattering coefficients) of the food samples. In this research, a spatially-resolved spectroscopy setup based on a fiber-optic probe was developed for acquiring spatially-resolved diffuse reflectance of five model foods with different designed microstructures and compositions in the range 400 – 1100 nm. A model for light propagation in turbid media based on diffusion approximation for solving the radiative transport equation was employed to derive optical properties (absorption and reduced scattering coefficients) of these model foods. The accuracy of this light propagation model was validated on solid phantoms with known optical properties. Results of solid phantoms indicated that diffusion equation is sufficiently accurate for modeling light propagation in the investigated samples. Derived reduced scattering coefficients µs’ of the model foods obviously showed a logical correlation with the corresponding microstructures of the model foods analyzed by optical microscopy. Estimated absorption coefficients µa were also in good agreement with the designed ingredients of these model foods. The research results clearly support the potential of spatially-resolved spectroscopy for non-destructive food quality inspection and process monitoring in the food industry.


Sensing Technologies for Biomaterial, Food, and Agriculture 2013 | 2013

Spatially resolved spectroscopy for nondestructive quality measurements of Braeburn apples cultivated in sub-fertilization condition

Nghia Nguyen Do Trong; Chyngyz Erkinbaev; Bart Nicolai; Wouter Saeys; Mizuki Tsuta; Josse De Baerdemaeker

A contact spatially resolved spectroscopy (SRS) setup based on a fiber-optics probe in the Vis/NIR range (400-1000 nm) was developed, calibrated, and validated for its measurements and optical properties estimation by means of a metamodeling method on a set of liquid optical phantoms. Thirty Braeburn apples cultivated in sub-fertilization condition were harvested and measured before and after shelf-life storage (2 weeks at 18 °C) by the setup and were analyzed for quality attributes (firmness and soluble solids contents (SSC)) by destructive reference methods. Estimated optical properties (absorption and reduced scattering coefficients) acquired from SRS measurements at the beginning and the end of the shelf-life indicated changes in chemical composition of the apples. Partial Least Squares Regression (PLSR) was employed to construct calibration models relating the estimated optical properties to the reference quality attributes. The constructed PLS models based on the absorption coefficient spectra gave good prediction performance for the quality attributes of the apples in the validation set with correlation coefficients r of 0.901 and r of 0.844, respectively for SSC and firmness. The obtained results clearly show the potential of the SRS measurements for nondestructive quality evaluation of apples.


2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010 | 2010

Optical Characterization of Biological Material: A Multiscale Approach

Wouter Saeys; Nghia Nguyen Do Trong; Rodrigo Watté; Mizuki Tsuta; Herman Ramon; Bart Nicolai

The change of Vis/NIR radiation when propagating through biological material is the result of a complex process of molecule-specific absorptions and multiple light scattering caused by the interaction of the photons with the microstructure. In addition, many biological products, such as fruit or skin tissue are characterized by a layered structure. Therefore, multiple measurements are needed to separate the information on the different layers. In this research, reflectance measurements at different distances from the incident light beam are combined with multiscale light propagation models to extract the compositional and microstructure properties of biological products. First, the biological material is modelled at the macroscale (~mm) as a set of uniform layers, where the light propagation in each layer is defined by 3 bulk optical properties. Using these 3 bulk properties the reflectance spectra at different distances from the incident light beam are calculated by Monte Carlo simulations for the radiative transport equation.


Proceedings of SPIE | 2014

Measurement of the optical properties of rat brain tissue using contact spatially resolved spectroscopy

Barbara Gysbrechts; Nghia Nguyen Do Trong; Ling Wang; Henrique Cabral; Zaneta Navratilova; Francesco P. Battaglia; Wouter Saeys; Carmen Bartic

Nowadays, biophotonics is widely used in neuroscience. The effectiveness of biophotonic techniques, such as fluorescence imaging and optogenetics, is affected by the optical properties of the examined tissue. Therefore, knowledge of these properties is essential to carefully plan experiments. Mice and rats are widely used in neuroscience studies. However, reports about optical properties of their brains are very rare. We measured optical absorption μa and reduced scattering μ’s coefficients of native rat brain in the visible and near-infrared wavelength region, using contact spatially resolved spectroscopy (SRS). In this study, we estimate μa and μ’s for the rat cortex and discuss their stability in time. Additionally, variations in optical properties within and between samples were characterized. The results extend the range of known optical properties for the rat cortex, especially in the visible range, relevant to optogenetics. μa and μ’s are stable within a time span of four hours, and show low variation in and between brain samples. This indicates that a suitable protocol was used to estimate optical properties of rodent brain tissue. Since contact SRS is a non-destructive method, this technique could be used also to measure μa and μ’s in living animals. Moreover, the probe has small dimensions, allowing the characterization of optical properties in different structures of the brain.

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Wouter Saeys

Katholieke Universiteit Leuven

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Bart Nicolai

Katholieke Universiteit Leuven

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Mizuki Tsuta

National Agriculture and Food Research Organization

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Josse De Baerdemaeker

Katholieke Universiteit Leuven

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Chyngyz Erkinbaev

Katholieke Universiteit Leuven

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Els Herremans

Katholieke Universiteit Leuven

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Pieter Verboven

Katholieke Universiteit Leuven

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Rodrigo Watté

Katholieke Universiteit Leuven

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Ben Aernouts

Katholieke Universiteit Leuven

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Eva Verhoelst

Katholieke Universiteit Leuven

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