Norman K. Wood

Incisional Biopsy and Seeding in Hamster Cheek Pouch Carcinoma

Surgical blades were contaminated by doing incisional biopsies on DMBA-induced cheek pouch carcinomas in hamsters. Contaminated blades were then used to: (1) make cytologic smears; (2) make incisions in the mucosal surface of the lower lip; (3) make incisions in the ventral surface of the tongue; (4) make incisions in the untreated left cheek pouch; and (5) make incisions in the pre-shaved back. One group of animals was killed three hr post-surgery. The second group was killed seven wk post-surgery. Tissues were excised and processed for microscopic study. Malignant cells were found in many of the incision sites of animals from the first group. In the animals of the second group, several tumors growing from the transplanted tumor cells were observed in the incision sites of the lower lips and the tongue. None was observed in the incision sites of the left cheek pouch or the back. Cervical lymph node metastases were found in approximately 50% of the animals of the second group.

In vivo autoradiographic study of relative penetrating abilities ofaqueous 2% parachlorophenol and camphorated 35% parachlorophenol

The in vivo diffusibility of camphorated 35% parachlorophenol (PCP) and aqueous 2 % PCP was compared by autoradiographic evaluation. Aqueous 2% PCP showed complete dentinal tubule penetration at a clinically equivalent dosage while camphorated 35% PCP was limited to a mean dentinal tubule penetration of 0.58 mm, or less than a fifth of the pulpoperiodontal distance. The results of this study substantiate the findings of a previous in vitro investigation and suggest that the use of camphorated 35% PCP be discontinued and replaced by an aqueous PCP preparation in routine endodontic therapy.

Autoradiographic studies of the intracanal diffusion of aqueous and camphorated parachlorophenol in endodontics

Abstract Studies of the penetrability of radio-labeled aqueous parachlorophenol and camphorated parachlorophenol were conducted by means of microscopic and autoradiographic techniques. The results indicated that aqueous parachlorophenol penetrated into the dentin from the pulp chamber and root canal and traveled at least to the cementodentinal junction, whereas the camphorated parachlorophenol did not.

Hamster Cheek Pouch Carcinoma: Effect of Incision and Cortisone on Growth, Invasion, and Metastasis

After cheek pouch carcinomas were induced in hamsters by the application of dimethylbenzanthracene (DMBA) to the right pouch for 13 weeks, the animals were divided into four groups and observed for seven more weeks. The control group received no further treatment, two experimental groups had incisional biopsies performed on tumors in their pouches, one of these groups also received injections of cortisone throughout the 20-week experimental period, and a fourth group received cortisone only. The wet weights of the cancerous cheek pouches were determined, and the submandibular and parotid salivary glands with associated cervical lymph nodes, the lungs, and the liver were examined with light microscopy. The cancerous pouches of the animals that received cortisone weighed significantly less than those of animals that received no cortisone but had incisional biopsies of the tumors. There was no significant difference in the degree of histodifferentiation of the tumors among the four groups. The animals in the two groups that received cortisone had significantly more tumors that were invasive than did the animals that did not receive cortisone. Cervical lymph node metastasis occurred in 21% to 38% of the animals but was not significantly different in the four groups. Distant metastases to the lungs or the liver were not found. Incisional biopsy of the tumors stimulated local growth of the cheek pouch tumors, and systemic cortisone administration produced more invasive cheek pouch tumors.

An unusual sequela of a gunshot wound of the face

Summary This report describes the case of a patient with a painful abscess of the body of the tongue. Roentgenograms revealed an upper right molar tooth in the tongue. Three years previously the patient had been shot in the right cheek with a 32-caliber bullet. The bullet apparently struck the molar on its buccal surface. The molar then became a secondary missile and penetrated to some depth into the tongue, where it remained unnoticed for almost 3 years before infection of the tongue led to its detection. The role of teeth as secondary missiles in gunshot wounds of the face is discussed.

An ancient “tumour” from Pre-Columbian Chile

A case of an expansile facial lesion discovered in the more than 600 years old skeletal remains of a 22-25-year-old female is discussed. Gross, radiographic and histological studies were accomplished on the specimen. Although a definitive diagnosis could not be derived because of the age and condition of the specimen, differential diagnoses are discussed and a probable diagnosis rendered.

Radioautographic Labeling in Placentas and Fetal Palatal Shelves After Maternal Injections of Tritiated Cortisone in A/Jax Mice

Tritiated cortisone was injected into pregnant A/Jax mice after a loading dose of cortisone on day 12 of pregnancy. Radioautographic studies of 160 placentas and fetal palatal shelves revealed: (1) significant labeling in all placental and fetal palatal tissue; (2) a variation in degree of labeling of fetal palates from different mothers; (3) no variation of degree of labeling in proximal or distal fetuses; and (4) a positive correlation in the degree of labeling in corresponding placentas and shelves.

A condylar tumor from pre-Columbian Chile: A case report

A case of an expansile, multilocular lesion in a condyle recovered in skeletal remains more than 700 years old is discussed. Gross, radiologic, and histologic studies were accomplished on the specimen. Although a definitive diagnosis could not be arrived at because of the age of the specimen, differential diagnoses are presented.