Patrick D. Toto

Reactivity of Blood Group Substances of Neoplastic Oral Epithelium

Histologic sections of 15 biopsy samples of normal oral mucosa and 8 biopsy samples of malignant oral carcinoma from patients with A, B, or AB blood groups were treated with anti-A or anti-B human antiserum and then with fluorescein isothiocyanate-bound goat antihuman globulin. Normal cells fluoresced intensely. Fluorescence of malignant cells was diminished or lost.

Detection of human papovavirus antigen in oral papillary lesions

The papillary lesions of oral verruca vulgaris, condyloma acuminatum, and verrucous carcinoma were examined for the presence of papovavirus antigens by the peroxidase-antiperoxidase immunohistochemical methods. The antigens could be detected in the nuclei of cells only in the stratum granulosum and stratum corneum in some cases of verruca vulgaris and condyloma acuminatum but in no case of verrucous carcinoma.

Radioactive Labeling Index of the Periodontal Ligament in Aging Rats

Fifteen rats, 10 to 400 days old, were given injections of tritiated thymidine. The percentage of labeled cells in the periodontal ligament of the maxillary first molar decreases with age. Functional occlusion reduces the numbers of cells synthesizing deoxyribonucleic acid.

Kinetics of Rat Molar Pulp Cells at Various Ages

SYNOPSIS IN INTERLINGUA CINETICA DE CELLULAS DE PULPA MOLAR AB RATTOS A VARIE ETATES.—Le synthese de acidos desoxyribonucleic esseva studiate in le pulpa molar de rattos de etates de inter 10 e 100 dies. Le distribution de frequentia del cellulas de tissu conjunctive in stato de synthese de acido desoxyribonucleic esseva determinate per marcation del nucleos de tal cellulas con thymidina a tritium con le preparation subsequente de autoradiogrammas. Le cellulas de pulpa manifesta synthese de acido desoxyribonucleic a transverso un periodo de 400 dies, e-per consequente—reflecte un continue turnover de cellulas. Le synthese de acido desoxyribonucleic es plus grande a 10 e 15 dies que a ulle altere tempore. Isto coincide con le formation de radices e le eruption. Le camera pulpal reduce su magnitude con le avantiamento del etate, sed illo augmenta su densitate cellular.

Aging in Human Attached Gingival Epithelium

The mitotic index of human gingival epithelium is not significantly different in men 18 to 22 years of age and in men 58 to 64 years of age. There is a significant increase in the cell density of the gingival epithelium and a significant decrease in connective tissue cells in older men.

Effect of Age Changes on the Premitotic Index in the Periodontium of Mice

Comparative autoradiographic studies of the periodontium of 60- and 300-day-old mice show that young mice not only have a greater number of cells but also a greater number of regenerable cells than do old mice. The time required for cells to double in number is less in the young mouse.

Labeling Index and Cell Density of Aging Rat Oral Tissues

Age changes were detected in oral epithelium, dental pulp, and periodontal ligament in the Simonsen rat.

Renewal of Cell Population in Palate and Tongue Epithelia of Mice

The generation cycle of epithelium of the palate in 60-day-old mice has been studied by employing the mitotic-index method of calculations. It was reported to be 24 days for the lateral area and 25 days for the central area of the palate.1 In another study, the renewal of epithelium of the tongue has been estimated to be 100 hours in 300-dayold mice, as determined by a combination of the stage-duration index and partial direct graphic methods.2 The direct graphic method for determination of the generation cycle is more reliable and has been employed in the studies of the gut epithelium in mice., 4 In the present investigation, this method has been used not only to determine the renewal time of the epithelium of the tongue but also of the palate epithelium in 60-day-old mice. This report is a part of the general study of the effect of aging on the generation cycle of the oral epithelium in mice.

Labeling Index and Cellular Density in Palatine Shelves of Cleft-Palate Mice

Cortisone-induced cleft-palate A/Jax fetal mice were administered tritiated thymidine by intraperitoneal injection of the mothers. Labeled mesenchymal cells of the palatine shelves showed a significant reduction. Also, there was reduced intercellular substance indicated by increased cell density.

Histogenesis of Osteoclasts

SYNOPSIS IN INTERLINGUA HISTOGENESE DE OSTEOCLASTAS.— Rattos blanc esseva subjicite a duo injectiones de 200 unitates de Parathormon con un intervallo de 12 horas. Tres horas post le prime injection, thymidina a tritium esseva administrate. Autoradiogrammas del maxillas esseva preparate ab specimens colligite inter hora 6 e hora 72 a partir del comenciamento del experimento. Marcate osteoplastas esseva trovate post hora 18. Illos se formava per le fusion de libere cellulas de tissu conjunctive del periodontio e del medulla. Le osteoclastas dispareva ab le lacunas inter hora 48 e hora 72. Le apparition de marcate osteocytos a iste tempore monstrava que etiam illos habeva lor origine in libere cellulas conjunctive occupante le spatio create per lacunas de Howship.