Ogino Shigeo
Sumitomo Chemical
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Publication
Featured researches published by Ogino Shigeo.
Gene | 1986
Kishimoto Fumitaka; Gomi Hideyuki; Kanaoka Masaharu; Nakatani Tomoyuki; Ito Akira; Katoh Tomoko; Agui Hideo; Sumida Seizo; Ogino Shigeo
Abstract Human epidermal growth factor (urogastrone; UG) is a 53-amino acid polypeptide hormone. A 192-bp DNA fragment containing the coding sequence for methionyl UG (Met-UG) and the ribosome-binding site (RBS) was chemically synthesized and placed downstream from the promoter for the Escherichia coli outer-membrane lipoprotein gene (Ipp) on a plasmid. E. coli cells harboring the plasmid directed the synthesis of Met-UG at 10 2 -10 2 molecules per cell. Next, the coding sequence for Met-UG was inserted in a runaway-replication plasmid and expressed under the control of the lpp promoter and the RBS derived from bacteriophage Mu cII gene. Upon heat induction, the cells harboring the recombinant plasmid synthesized 10 5 molecules of Met-UG per cell.
Archive | 1979
Yanagi Hideki; Ogino Shigeo
Archive | 1986
Kishimoto Fumitaka; Ito Akira; Gomi Hideyuki; Yasukui Hideo; Ogino Shigeo; Yoda Koji; Yamazaki Masakari; Tamura Gakuzo
Archive | 1979
Fukui Masaru; Ogino Shigeo; Yamamoto Hisao
Archive | 1985
Kishimoto Fumitaka; Kaneoka Shiyouji; Nakatani Tomosuke; Sumida Seizou; Ogino Shigeo
Archive | 1984
Nakanishi Tooru; Noguchi Hiroshi; Ogino Shigeo
Archive | 1984
Nakanishi Tooru; Yanagi Hideki; Ogino Shigeo
Archive | 1983
Hozumi Tatsunobu; Nakayama Chikao; Yanagi Hideki; Ogino Shigeo
Archive | 1987
Jigami Yoshifumi; Uemura Hiroshi; Muraki Michirou; Tanaka Hideaki; Nakazato Satoshi; Kishimoto Fumitaka; Yasukui Hideo; Ogino Shigeo
Archive | 1986
Muraki Michirou; Chigami Yoshifumi; Tanaka Hideaki; Kishimoto Fumitaka; Yasukui Hideo; Ogino Shigeo; Nakazato Satoshi
