Oliver C. Schultheiss

Salivary testosterone, cortisol, and progesterone: Two-week stability, interhormone correlations, and effects of time of day, menstrual cycle, and oral contraceptive use on steroid hormone levels ☆

With salivary assessment of steroid hormones increasing, more work is needed to address fundamental properties of steroid hormone levels in humans. Using a test-retest design and radioimmunoassay assessment of salivary steroids, we tested the reliability of testosterone, cortisol, and progesterone levels across two weeks, as well as the effects of oral contraceptives, menstrual cycle phase, and time of day on steroid hormone levels. Testosterone and cortisol were found to be highly reliable in both sexes. Progesterone was found to be reliable after collapsing across sex. Oral contraceptive use was associated with lower levels of testosterone, but did not affect cortisol. Contrary to expectations, oral contraceptives also did not affect progesterone. Menstrual cycle was found to affect levels of progesterone, but not testosterone or cortisol. Time of day had an effect on cortisol, on progesterone only at one testing time, and no effect on testosterone. We explored the interhormone correlations among testosterone, progesterone, and cortisol. All three hormones were positively correlated with one another in men. In women, progesterone was positively correlated with testosterone and cortisol, but testosterone and cortisol were uncorrelated.

Assessment of Implicit Motives With a Research Version of the TAT: Picture Profiles, Gender Differences, and Relations to Other Personality Measures

Four hundred twenty-eight participants wrote imaginative stories in response to 6 picture cues of a research version of the Thematic Apperception Test (TAT; Morgan & Murray, 1935). Story protocols were coded for n (need) Power, n Achievement, and n Affiliation using Winters (1991) integrated scoring system that provided detailed information about the motive profiles of individual picture cues. In general, picture cues differed strongly from each other with regard to how many scorable instances of power, achievement, or affiliation imagery they elicited. The n Affiliation, but not n Power, n Achievement, or activity inhibition-a measure of impulse control-was found to be higher in (a) women than in men and (b) individuals tested in a group than in individuals tested individually. TAT motive measures showed no significant overlap with questionnaire measures of motivational orientation (German Personality Research Form; Stumpf, Angleitner, Wieck, Jackson, & Beloch-Till, 1985) or traits (German NEO-Five-Factor Inventory; Borkenau & Ostendorf, 1993).

Testosterone is positively associated with risk taking in the Iowa Gambling Task

The association between testosterone and economic risk is not well-understood and is understudied. The present study aimed to further characterize what if any relationship testosterone has with risky economic decisions. To do so, 154 participants (78 men) completed the Iowa Gambling Task (IGT) (Bechara et al., 1994) and also provided saliva samples, which were assayed for endogenous testosterone levels using radioimmunoassay. High-levels of endogenous testosterone were associated with choosing less frequently from advantageous IGT decks of cards, indicating greater risk taking. The data showed that the effects of testosterone on IGT performance were similar for men and women. High-testosterone women and high-testosterone men made riskier choices than their low-testosterone counterparts of the same sex, and this effect was pronounced in women. Thus, high levels of testosterone are associated with willingness to incur greater risk in both sexes.

Implicit Power Motivation Predicts Men's Testosterone Changes and Implicit Learning in a Contest Situation

This study tested the hypothesis that implicit power motivation moderates mens testosterone responses to victory or defeat in a contest situation. It also explored to what extent postvictory testosterone increases are associated with enhanced implicit learning of behavior instrumental for winning a contest. Salivary testosterone levels were assessed in 66 male adults several times before and after a contest whose outcome (winning or losing against a competitor on an implicit learning task) was varied experimentally. Among participants low in activity inhibition, a measure of impulse control, the power motive was a significant positive predictor of testosterone increases (15 min postcontest; r = 0.71, P = 0.01) and implicit learning (r = 0.68, P < 0.05) after a victory, whereas it was a significant negative predictor of implicit learning (r = -0.58, P = 0.01) but not of testosterone increases (r = -0.08, ns) after a defeat. Moreover, among participants low in activity inhibition testosterone increases were associated with enhanced implicit learning (r = 0.38, P < 0.05) and there was statistical evidence that in winners testosterone increases mediated the effect of power motivation on implicit learning. Participants high in activity inhibition did not display this pattern of results.

Salivary cortisol changes in humans after winning or losing a dominance contest depend on implicit power motivation

In two studies, one with an all-male German sample and the other with a mixed-sex U.S. sample, subjects competed in pairs on reaction time-based cognitive tasks. Participants were not aware that contest outcome was experimentally varied. In both studies, implicit power motivation, defined as the non-conscious need to dominate or have impact on others, predicted changes in salivary cortisol from before to after the contest. Increased cortisol post-contest was associated with high levels of power motivation among losers but with low levels of power motivation among winners, suggesting that a dominance success is stressful for low-power individuals, whereas a social defeat is stressful for high-power individuals. These results emerged only in participants tested in the afternoon, possibly because of greater variability in cortisol in the morning due to the rapid decline after the morning peak. These studies add to the evidence that individual differences greatly influence whether a social stressor like losing a contest activates the HPA axis in humans.

Assessing implicit motives in U.S. college students: effects of picture type and position, gender and ethnicity, and cross-cultural comparisons.

We assessed implicit needs for power, achievement, and affiliation in 323 U.S. college students using a Picture Story Exercise (PSE; McClelland, Koestner, & Weinberger, 1989) consisting of 6 picture cues and Winters (1994) content coding system. Picture cues differed markedly in the amount of motive imagery they elicited and picture motive profiles closely resembled those reported by Schultheiss and Brunstein (2001) for a German student sample. Picture position influenced the expression of power and affiliation motivation, with affiliation motivation being most strongly expressed at the beginning and power motivation being most strongly expressed in the middle of the PSE. Women had higher affiliation motive scores than men. Asian Americans had higher affiliation motive scores than Whites, and African Americans had higher levels of achievement motivation than Asian Americans or Whites. PSE motive measures showed little or no overlap with questionnaire measures of impulsivity and anxiety (Behavioral Inhibition System–Behavioral Activation System scales; Carver & White, 1994) or specific motivational orientations (Personality Research Form; Jackson, 1984). Comparisons with Schultheiss and Brunsteins (2001) German sample indicate that U.S. students have higher achievement motivation and lower power motivation and activity inhibition scores than German students.

Basal testosterone moderates responses to anger faces in humans

Prior research [van Honk J, Tuiten A, Verbaten R, van den Hout M, Koppeschaar H, Thijssen J, de Haan E. Correlations among salivary testosterone, mood, and selective attention to threat in humans. Horm Behav 1999;36(1):17-24; van Honk J, Tuiten A, Hermans E, Putman P, Koppeschaar H, Thijssen J, Verbaten R, van Doornen L. A single administration of testosterone induces cardiac accelerative responses to angry faces in healthy young women. Behav Neurosci 2001;115(1):238-42.] showed relationships in humans between testosterone (T) and vigilance to facial expressions of anger, which are considered signals of an impending dominance challenge. In Study 1, we used a differential implicit learning task (DILT) (see [Schultheiss OC, Pang JS, Torges CM, Wirth MM, Treynor W. Perceived facial expressions of emotion as motivational incentives: evidence from a differential implicit learning paradigm. Emotion 2005;5(1):41-54.]) to investigate the degree to which subjects find anger faces reinforcing. In the DILT, separate sequences of actions were paired with presentations of anger faces, neutral faces or a blank screen. After training, performance on the three sequences was measured in the absence of face stimuli. Saliva was collected for T measurement. Higher T predicted better learning on sequences paired with sub-threshold (i.e., presented too fast for conscious awareness) anger faces, suggesting that T is related to reinforcing qualities of these faces. In Study 2, we examined whether morning or afternoon T better predicted attention and vigilance to anger faces. Participants were tested at 9:00 and 15:00. At each session, saliva was collected for T measurement, and participants completed a Stroop task and a dot-probe task [Mogg K, Bradley BP, Hallowell N. Attentional bias to threat: roles of trait anxiety, stressful events, and awareness. Q J Exp Psychol A 1994;47(4):841-64.] with facial expression stimuli. Morning (peak) T was a better predictor of responses to anger faces than afternoon T. Morning T predicted greater Stroop-like interference to sub-threshold anger faces, as well as attentional orienting away from sub-threshold anger faces. These effects were not present for joy faces or for supraliminal anger faces. T may generally decrease aversion to threatening stimuli, and/or may specifically facilitate approach towards signals of dominance challenge.

Implicit motives and gonadal steroid hormones: effects of menstrual cycle phase, oral contraceptive use, and relationship status.

Implicit motives for power and affiliation, salivary levels of testosterone, estradiol, and progesterone, and relationship status were measured in 18 normally cycling (NC) women, 18 women using oral contraceptives (OC), and 18 men at three assessments, corresponding to the menstrual, midcycle, and premenstrual phases of womens menstrual cycle. NC and OC women had elevated levels of affiliation motivation and decreased levels of power motivation at midcycle. Power motive changes were particularly pronounced in NC women across cycle phases. OC women and participants not engaged in an intimate relationship had significantly heightened levels of affiliation motivation, averaged across all cycle phases. Testosterone and power motivation, both averaged across all cycle phases, were positively correlated in men and in single women, but not in women engaged in an intimate relationship. Averaged levels of estradiol and power motivation were positively correlated in engaged women, but not in single women or men. Averaged levels of progesterone and affiliation motivation were negatively correlated in men, and there was evidence for a positive association between luteal affiliation motivation and periovulatory and luteal progesterone in NC women. This study therefore provides evidence that implicit motivational states fluctuate across the menstrual cycle, that the power motive is associated with testosterone and, in women, with estradiol, and that the affiliation motive and progesterone are associated in different ways in men and NC women.

Methodological and Dispositional Predictors of Congruence Between Implicit and Explicit Need for Achievement

In this research, the authors questioned the statistical independence of implicit and explicit motives. Their first aim was to identify a methodological factor that may have weakened implicit—explicit motive correlations in past research. Their second aim was to identify personality traits that moderate implicit—explicit motive congruence. They found that implicit and explicit need for achievement (nAch) are significantly correlated, but only if the implicit and explicit measures are matched in content. Three traits were found to uniquely moderate the relationship between implicit and explicit nAch: private body consciousness, self-monitoring, and preference for consistency. These findings indicate that implicit and explicit nAch are systematically related and suggest that some individuals may use implicit nAch as a foundation for the development of explicit nAch.

Endogenous testosterone levels are associated with amygdala and ventromedial prefrontal cortex responses to anger faces in men but not women

Testosterone moderates behavioral and physiological responses to the emotion anger. However, little is known about the effects of testosterone in the human brain in the context of the perception of anger. We used fMRI to measure BOLD responses to anger faces in the amygdala and ventromedial prefrontal cortex (vmPFC) as a function of endogenous testosterone levels in 24 participants (10 men). In one task, participants passively viewed anger faces and neutral faces and in another task, participants engaged in an oddball task while viewing anger and neutral faces. Mens, but not womens, amygdala BOLD response to anger faces was negatively correlated with their endogenous testosterone levels in both tasks. Mens, but not womens, vmPFC BOLD response to anger faces was positively correlated with their endogenous testosterone levels in the passive-viewing task. In men, amygdala and vmPFC BOLD responses to anger faces were negatively associated. Our results extend past research by documenting associations between endogenous testosterone levels and BOLD responses to anger faces in the amygdala and vmPFC in men, and our results also support research that documents negative associations between amygdala and vmPFC activity.