Olivier Bethenod
Institut national de la recherche agronomique
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Featured researches published by Olivier Bethenod.
Environmental Pollution | 2012
Ludger Grünhage; Håkan Pleijel; Gina Mills; Jürgen Bender; Helena Danielsson; Yvonne Lehmann; Jean-François Castell; Olivier Bethenod
Field measurements and open-top chamber experiments using nine current European winter wheat cultivars provided a data set that was used to revise and improve the parameterisation of a stomatal conductance model for wheat, including a revised value for maximum stomatal conductance and new functions for phenology and soil moisture. For the calculation of stomatal conductance for ozone a diffusivity ratio between O(3) and H(2)O in air of 0.663 was applied, based on a critical review of the literature. By applying the improved parameterisation for stomatal conductance, new flux-effect relationships for grain yield, grain mass and protein yield were developed for use in ozone risk assessments including effects on food security. An example of application of the flux model at the local scale in Germany shows that negative effects of ozone on wheat grain yield were likely each year and on protein yield in most years since the mid 1980s.
Chemosphere | 2012
Rafiq Ahmad; Yasmine Zuily-Fodil; Chantal Passaquet; Olivier Bethenod; Romain Roche; Anne Repellin
Maize plants (Zea mays L. cv. NK Perform) were exposed to O(3)-enriched air, using a new field fumigation system. Transcriptional changes for three type II-metacaspase genes were studied in the leaves (ranks 10 and 12), using quantitative real-time PCR. Global metacaspase activity was measured using metacaspase-specific synthetic tripeptide Boc-GRR-AMC. Aging had little effect on mRNA accumulation whereas four to six-fold increases were observed for the most O(3)-responsive type II metacaspase genes, in the older leaves 10. Global metacaspase activity increased by 257% and 333% in leaves 12 and 10, respectively, in response to the highest cumulated concentration. In non-fumigated plants, metacaspase activity progressively increased over the course of the experiment and always was higher in the older leaves 10. Together, these results suggest that metacaspase-mediated proteolysis is a crucial step in leaf responses to both O(3) and age-mediated senescence.
Archive | 1990
Olivier Bethenod; François Tardieu
Rain water use efficiency can be defined by the ratio of dry matter production (DMP) to rainfall (R). It can be written as the product of three ratios (i) the ratio of transpiration (E) to rainfall, (ii) that of CO2 net assimilation to transpired water (An/E), (iii) that of the conversion of this photosynthesis into dry matter.
Chemosphere | 2014
Rafiq Ahmad; Yasmine Zuily-Fodil; Chantal Passaquet; Olivier Bethenod; Romain Roche; Anne Repellin
Among the different classes of endoproteases, cysteine proteases are consistently associated with senescence, defense signaling pathways and cellular responses to abiotic stresses. The objectives of this work were to study the effects of various concentrations of ozone on gene expression and enzymatic activity for papain-like cysteine proteases (PLCPs), in the leaves of maize plants grown under field conditions. Leaves from ranks 12 and 10 (cob leaf) were harvested regularly over a long-term artificial ozone fumigation experiment (50 d). Tissues were tested for transcriptional and activity changes concerning cysteine proteases, using qRT-PCR for the newly identified ozone-responsive PLCP gene (Mor-CP) and synthetic oligopeptide Boc-Val-Leu-Lys-AMC as a PLCP-specific substrate, respectively. Results showed that developmental senescence induced a significant and progressive rise in CP activity, only in the older leaves 10 and had no effect on Mor-CP gene expression levels. On the other hand, ozone dramatically enhanced Mor-CP mRNA levels and global PLCP enzymatic activity in leaves 12 and 10, particularly toward the end of the treatment. Ozone impact was more pronounced in the older leaves 10. Together, these observations concurred to conclude that ozone stress enhances natural senescence processes, such as those related to proteolysis.
Archive | 1990
Olivier Bethenod; Jean-Paul Lhomme; Nader Katerji
CO2 flux and H2O flux pass through stomata. Stomatal aperture depends on both internal and external factors.
Crop Science | 1994
Nader Katerji; François Tardieu; Olivier Bethenod; Philippe Quetin
Theoretical and Applied Climatology | 2000
Olivier Bethenod; Nader Katerji; R. Goujet; J.M Bertolini; G. Rana
Agronomie | 2002
Albert Olioso; Isabelle Braud; André Chanzy; Jérôme Demarty; Yannick Ducros; Jean-Claude Gaudu; Enrique Gonzalez-Sosa; Elizabet Lewan; Olivier Marloie; Catherine Ottlé; Laurent Prévot; Jean-Louis Thony; Hervé Autret; Olivier Bethenod; Jean-Marc Bonnefond; Nadine Bruguier; Jean-Paul Buis; Jean-Christophe Calvet; Vicente Caselles; Habiba Chauki; César Coll; Christophe François; Robert Goujet; Raymond Jongschaap; Yann Kerr; Christine King; Jean-Pierre Lagouarde; Jean-Paul Laurent; Patrice Lecharpentier; John Mcaneney
Agronomie | 1990
François Tardieu; Nader Katerji; Olivier Bethenod
Plant Biology | 2007
Louis Leitao; Olivier Bethenod; Jean-Philippe Biolley