Ondřej Bučko

Effect of diet supplemented with propolis extract and probiotic additives on performance, carcass characteristics and meat composition of broiler chickens

The present research focused on the effects of propolis extract and probiotic preparation based on Lactobacillus fermentum (1 × 10 9 CFU per 1 g of bearing medium) on performance, carcass characteristics and meat composition of broiler chickens. The experiment was performed with 360 one day-old Ross 308 broiler chicks of mixed sex. The chicks were randomly allocated into 3 groups (n = 120 pcs chicks per group), namely, control (C) and experimental (E1, E2). Each group consisted of 3 replicated pens with 40 broiler chickens per pen. The experiment employed a randomized design, and dietary treatments were as follows: 1. basal diet with no supplementation as control (group C), 2. basal diet plus 400 mg propolis extract per 1 kg of feed mixture (group E1), 3. basal diet plus 3.3 g probiotic preparation added to drinking water (group E2). Besides, the groups were kept under the same conditions. Fattening period lasted for 42 days. Feed mixtures were produced without any antibiotic preparations and coccidiostats. As regards performance of broilers, all the investigated parameters were improved after addition of the supplements, especially after probiotic supplementation. However, neither propolis extract nor probiotic in diet of broiler chickens had any significant effect ( p ≥0.05) on performance. Meat composition was evaluated as proximate composition (dry matter, crude protein, fat and ash), cholesterol content and energy value in the most valuable parts of chicken meat (breast and thigh muscles). The statistically significant results ( p ≤0.05) were attained in fat, ash and cholesterol content, as well as energy value in both breast and thigh muscles after the propolis supplementation. To sum up, the present study demonstrated the promising potential of propolis extract and probiotic to enhance the performance, carcass characteristics and meat composition under conditions of the experiment with, however, statistical significance of results in a few parameters. Normal 0 21 false false false SK X-NONE X-NONE

The Behaviour of the Pigs Housed in Hot Climatic Conditions

The effect of high temperature on the behaviour of growing-finishing pigs was studied. The pigs were housed in a climate controlled chamber, the air temperature was kept constant at 30°C and the relative humidity was 32.5% during the whole 3 months. Aggressive behaviour and daily activities of the pigs were recorded during the light hours, from 6:00 till 18:00. Detailed observations showed that during the experiment, the pigs were most of the time lying (72%, P<0.001). Pigs spent more time lying on the floor without bedding (86.65%, P<0.01) compared to floor with bedding (13.35%, P<0.001). The second activity, which occurred most after lying was eating (16%, P<0.001) then standing (10%, P<0.001). From all observation activities, the least amount of time the pigs were sitting (1%) and moving (1%). Drinking and social contacts – aggression were less frequent. After the each week, a significant decrease in aggression was recorded (P<0.001), an aggressive contacts occurred mainly during the eating and drinking. The pigs were active with peaks in the morning and afternoon. This study confirms that the high temperature influences the behaviour of the animals; the pigs are not very active, the most of the time they are lying on the cool places without bedding.

Fatty Acids Profile of Intramuscular Fat in Light Lambs Traditionally and Artificially Reared

The quality of 40 carcasses of light lambs of the synthetic population of Slovak Dairy sheep from (a) artificial rearing (AR) and (b) traditional rearing (TR) was assessed on the basis of fatty acids profile of intramuscular fat (IMF). Lambs from AR in comparison with TR were of lower quality as assessed on the basis of fatty acids (FAs) profile. The content of conjugated linolic acid (CLA) in the fat of TR lambs was severalfold higher (0.749 vs. 0.193 g.100g-1 FAME, P<0.001) than in AR lambs. Similarly, the content of trans-vaccenic (TVA), a-linolenic (ALA), rumenic (RA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) was in TR lambs significantly higher (P<0.001) than in AR lambs (0.955 vs. 0.111; 0.715 vs. 0.251; 0.672 vs. 0.148; 0.352 vs. 0.061; 0.252 vs. 0.079 g.100g-1 FAME). In contrary, the content of linoleic acid (LA), the ratio of LA/ALA and n-6/n-3 in AR lambs was higher than in TR lambs (9.07 vs. 4.81 g.100 g-1 FAME; 39.11 vs. 6.80; 14.56 vs. 3.25, P<0.001). In TR lambs the content of n-3 PUFA and BCFA was significantly higher (P<0.001) than in AR lambs (2.08 vs. 0.84 and 1.95 vs. 0.45). The value of thrombogenic index was higher in AR lambs in comparison with TR lambs (1.44 vs. 1.31; P<0.05). Significant differences between FAs of IMF of ram lambs and ewe lambs were observed only in the case of arachidonic acid (P<0.05).

CHEMICAL COMPOSITION OF MUSCLE AFTER POLLEN APPLICATION IN NUTRITION OF BROILER CHICKENS

Principle purpose of this study was to monitor the chemical composition of breast and thigh muscular part of chickens of hybrid combination Ross 308 and to verify the differences due to use of pollen extract in feed mixture at a dose of 400 and 800 mg.kg -1 during the feeding of 42 days. We did not find significant differences (P≥0.05) in followed chemical compounds of thigh muscle with the skin and subcutaneous fat between the groups with and without application of pollen extract: in contents of water (68.49-70.12 g.100g -1 ), protein (18.82-18.98 g.100g -1 ), fat (9.96-11.53 g.100g -1 ) and in energy value (692.20-752.36 kJ.100g -1 ). Significant differences (P≤0.05) were found only in protein content of breast muscle between the control group (23.96 g.100g -1 ) and experimental group I (23.28 g.100g -1 ). Values of water content (73.97-74.32 g.100g -1 ), fat content (1.07-1.40 g.100g -1 ) and energy (441.65-446.64 kJ.100g -1 ) in breast muscle between the evaluated groups were balanced (P≥0.05). The results show that pollen extract at the concentration used in feed mixture did not effect basic chemical composition of the most valuable parts of the chicken Ross 308 carcass and we can apply it in their nutrition. Application at a dose of 800 mg.kg -1 seems to be more positive.

Comparison of fatty acid profile in the chicken meat after feeding with narasin, nicarbazin and salinomycin sodium and phyto-additive substances

ABSTRACT The purpose of this study was an experimental investigation and a statistical evaluation of the influence of various additives in feed mixtures of broiler chickens on fatty acids content and their ratio in breast and thigh muscles. First feed additive consisted of narasin, nicarbasin and salinomycin sodium, and other five additives were of phytogenic origin. In vivo experiment was realized on the poultry experimental station with deep litter breeding system. A total of 300 one-day-old hybrid chickens Cobb 500 divided into six groups were used for the experiment. The experimental period was divided into four phases, i.e. Starter, Grower 1, Grower 2 and Final, according to the application of commercial feed mixture of soy cereal type. Additive substances used in feed mixtures were different for each group. Basic feed mixtures were equal for all groups. Fatty acid profile of breast and thigh muscles was measured by the method of FT IR Nicolet 6700. Investigated additive substances in the feed mixtures did not have statistically significant effect on fatty acid content and omega-6/omega-3 polyunsaturated fatty acid (PUFA) ratio in breast and thigh muscles. Strong statistically significant relation between omega-6 PUFAs and total PUFAs were proved by experiment. A relation between omega-3 PUFAs and total PUFAs was found only in the group with Biocitro additive.

Sensory quality, colour and oxidative stability of cured cooked ham with propolis extract.

The effect of 0.06% propolis ethanol extract on the sensory quality, colour and oxidative stability of cured cooked ham was evaluated. Half of the experimentally processed hams treated with 0.06% ethanolic extract of propolis with ascorbic acid (EEP), only with ascorbic acid (AA) and control samples (C) were sliced and vacuum packaged. Samples were kept at 4 °C 21 days (sliced) respectively 20, 50 and 100 days (unsliced). The results revealed that all samples were characterized without any significant colour discrepancies. In general, the thiobarbituric acid value (mg malondialdehyde/kg) increased gradually in all samples examined, with a significantly lower (P <0.05) level for treated samples than for controls. The significantly lowest (P <0.05) sensory parameters in comparison to unsliced hams were observed in sliced hams packaged in vacuum. Sliced hams with EEP were characterized with significantly lowest (P <0.05) intensity of characteristic aroma. Undesirable taste was detected in control sliced hams after storage period. Significantly (P <0.05) more desirable taste of sliced hams was observed in those with only ascorbic acid in comparison with EEP. In our study was demonstrated that 0.06% ethanol extract of propolis positive affected oxidation stability and not negative affected others technological (pH, colour) and sensory characteristics of poultry meat product - cured cooked ham. Normal 0 21 false false false CS JA X-NONE

THE ANALYSIS OF THE CARCASS CHARACTERISTICS AND PHYSICAL-TECHNOLOGICAL QUALITY OF PORK AFTER THE USING DIET WITH THE ADDITION OF ORGANIC CHROMIUM AND SELENIUM

The aim of the study was to analyze the effect of selenium and combination of selenium with chromium in the feed mixture on carcass and meat traits of finishing pigs. A total 90 castrates (Large White) were divided into the three groups. The control group received basal diet (BD) without selenium and chromium; the experimental group (Se) 0.3 mg Se*kg-1 in the form of selenomethionine was added to the basal diet. Experimental group (SeCr) was fed basal diet with added selenomethionine plus chromium nicotinate (0.3 mg Se*kg-1 , 0.75 mg Cr*kg-1 ). We found that added selenomethionine to the feeding ration increased carcass length (P < 0.001). Technological parameters were affected by selenium: reduced pH24 of ham and drip loss of muscle longissimus thoracic after 7-day post mortem (P ≤ 0.01), and increased cooking loss in muscle abductor. Combination of selenium and chromium in the feeding ration significantly increased firmness of meat (Warner - Bratzler) in each muscle (P < 0.001). Cholesterol in muscles was lower (P < 0.01) in animals supplemented with a combination of selenomethionine and chromium. These results suggest that supplementation of organic selenium and chromium nicotinate in the feed had significant effect on carcass and meat quality of pigs.

Protein quality chicken meat after feeding with active substances of citrus fruits and diclazuril and salinomycin sodium

The purpose of this study was an experimental investigation of the influence of active substances obtained mainly from citrus fruits in the experimental feed mixtures, and diclazuril and salinomycin sodium in the control feed mixtures of broiler chickens on productive efficiency and protein quality of the breast and thigh muscles. In vivo experiment was carried out with hybrid chickens Cobb 500. Basic feed mixtures were equal a soy cereal type for experimental and control group. Indicators of productive efficiency were measured and calculated, and protein, lysine and methionine contents in the breast and thigh muscles were measured by the method of FT IR, Nicolet 6700. Active substances obtained mainly from citrus fruits confirmed a statistically significant (p ˂0.05) positive effect on the body weight gain; tended to slightly increase feed intake per bird, protein, energy, lysine and methionine intake per bird; slightly decrease feed intake per 1 kg of body weight gain, protein, energy, lysine and methionine intake per 1 kg of body weight gain; slightly increase protein efficiency ratio and energy efficiency ratio. Additive substances used in the feed mixtures did not have a statistically significant effect on protein, lysine and methionine contents in the breast and thigh muscles but displayed a strong positive, statistically significant relation between lysine and methionine in them.

The effect of the regular consumption of lard from fats of crossbreed Mangalitsa and breed of meat type pig on the lipid profile of consumers

The aim of this study was to evaluate the influence of consumption of lard processed from crossbreed of the original mangalitsa genotype and the breed of meat type pigs on selected biochemical parameters: total cholesterol (T-C), LDL cholesterol, HDL cholesterol and triacylglycerols (TAG). Twenty-nine probands participated in the study, of which 14 women and 15 men (aged 21 - 59) who consumed regularly lard four times a week during 5 weeks. By evaluating the lipid profile of probands, we observed elevated hypercholesterolemia at the beginning of the study in 43% women and 27% men. Statistically significant differences were recorded in this group of probands, where we recorded a decrease in TC of 0.55 mmol.L -1 in the whole sample of probands with p <0.05 probability in the 2 nd sample of study after 5 weeks of consumption of lard. Borderline high (up to 3.35 mmol.L -1 ) was found in 21% of women and 33% of men, and above LDL cholesterol was found in 79% of women and 60% of men. This high level of LDL cholesterol is considered a risk factor for the development of atherosclerosis. We can conclude from the results that regular consumption of lard has significantly reduced the total cholesterol levels, especially in women, this effect has been associated with lowering LDL cholesterol ( p <0.01) and lowering HDL cholesterol. Normal 0 21 false false false SK X-NONE X-NONE

Chemical composition of chicken meat after application of humic acid and probiotic Lactobacillus fermentum

The aim of the present study was analysed and evaluated chemical parameters of chicken breast and thigh muscles after addition of humic acids and probiotic into diet for broiler chicken. A total of 200 pcs Ross 308 broiler chickens were divided into 4 groups (n=50). The control group of chickens was fed with complete feed mixtures without any additives. Chickens in experiment groups were fed a diet containing: P1 (1% of humic acid), P2 (1% of humic acid and probiotic supplement Lactobacillus fermentum ) and P3 were fed with complete feed mixture containing combination of starter feed mixture (1. - 21. day) with coccidiostaticum Diclazuril and growth feed mixture (21. - 35. day) containing Salinomycinum sodium. Besides, the groups were kept under the same conditions. Fattening period lasted for 42 days. Chicken meat was analyzed for content of water, crude protein, fat and cholesterol. Based on the results, we can state that the application of humic acids or the combination of Humac Natur with probiotic did not affect the chemical composition of the breast muscle. In the breast muscle, the protein content in the experimental group P3 with the coccidiostat (22.98 g.100 g -1 ) was reduced ( p ≤0.05) compared to control group (23.42 g.100 g -1 ). In the case of thigh muscle was significantly higher content of fat and cholesterol ( p ≤0.05) in chickens feeding with addition of Humac Natur (fat - 9.08 g.100g -1 ; cholesterol - 0.86 mg.100g -1 ) and similar results were recorded in experimental group with combination of Humac Natur and probiotic (fat - 9.15 g.100g -1 ; cholesterol - 0.86 mg.100g -1 ) compared to control group (fat - 7.15 g.100g -1 ; cholesterol - 0.70 mg.100g -1 ). From a general point of view, we can recommend the application of Humac Natur, respectively combination Humac Natur with probiotics in feeding of broiler chickens Ross 308. 14.00 Normal 0 21 false false false EN-GB X-NONE X-NONE