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Effect of cholesterol loaded cyclodextrin on semen cryopreservation of Aksaray Malakli shepherd dogs of different ages

The objective of the study was to determine the effect of cholesterol-loaded cyclodextrin (CLC) on the quality parameters of semen from Aksaray Malakli Shepherd dogs of different age groups. Forty-eight male dogs were divided into 3 groupings according to their ages (young age (Y): ≤3 years, n: 20; middle age (M): 4-6 years, n: 20; old age (O): ≥7 years; n: 8). The sperm-rich portion of the ejaculate from each dog was divided into four aliquots and extended with either tris as a control (C) or tris loaded with 0.5, 1.0, and 1.5 mg/120 × 106 CLC as low (L), intermediate (I), and high (H) doses, respectively. Following equilibration for at least half an hour, the straws were frozen in nitrogen vapor and then stored in liquid nitrogen at least for 48 h. Later, the frozen straws were thawed in a water bath for spermatological evaluation. Significant differences were observed between different age groups in terms of the spermatological parameters (p < 0.05). The evidence suggests that increasing age is associated with poor in-vitro spermatological parameters and CLC was able to protect the acrosome integrity from cryo-damage during the freeze-thawing process. Better semen freezability characteristics were obtained at young ages, considering the overall parameters.

Dondurulmuş Boğa Spermalarında Spermatolojik Parametrelerin Konvansiyonel ve CASA (Bilgisayar Destekli Sperm Analiz) Yöntemiyle Değerlendirilmesi

Bu arastirmada, ithal ve yerli uretim spermalarin ulkemizde geleneksel yontemlerle faz-kontrast isik mikroskobu kullanilarak subjektif olarak yapilan spermatolojik muayenelerinin, CASA sistemiyle yapilan degerlendirmelerle karsilastirilmasi amaclandi. Farkli 20 bogaya ait dondurulmus spermalar (ithal ve yerli uretim) faz-kontrast isik mikroskobu yontemi (konvansiyonel yontem) ve bilgisayar destekli sperm analiz yontemi (CASA yontemi) ile muayene edildi ve baslica spermatolojik ozellikler yonuyle degerlendirildi. Ithal spermalarda muayene edilen orneklerin ortalama degerleri arasinda spermatozoa motilitesi, spermatozoa yogunlugu, anormal spermatozoa ve olu spermatozoa oranlari arasinda gozlenen farkliliklar istatistiksel acidan onemli oldu (P<0,05). Yerli spermalarda ise konvansiyonel yontemle numuneler arasinda saptanan spermatozoa yogunlugu yonuyle gozlenen farkliliklar ve CASA yontemiyle elde edilen spermatozoa motilitesi ve spermatozoa yogunlugu bakimindan gozlenen farkliliklar istatistiksel acidan onemli bulundu (P<0,05). Genel ortalama degerlere bakildiginda konvansiyonel ve CASA yontemiyle elde edilen sonuclara gore yalnizca spermatozoa yogunlugunda konvansiyonel yontemle onemli olcude daha yuksek veriler alindigi, spermatozoa motilitesi, anormal spermatozoa orani ve olu spermatozoa degerlerindeki farkliliklarin istatistiksel acidan onemsiz oldugu belirlendi (P<0,05). Her iki yontemle, belirlenen muayene parametrelerine iliskin bulgular karsilastirildi ve yontemlerin guvenilirlikleri ortaya konuldu.