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Featured researches published by Orhan Kankavi.


Biochemical Genetics | 2011

Expression of Biologically Active Human Interferon Gamma in the Milk of Transgenic Mice Under the Control of the Murine Whey Acidic Protein Gene Promoter

Haydar Bagis; Digdem Aktoprakligil; Cagatay Günes; Sezen Arat; Tolga Akkoc; Gaye Cetinkaya; Orhan Kankavi; Ali Cihan Taskin; Korhan Arslan; Munis Dundar; Vania L. Tsoncheva; Ivan Ivanov

Transgenic animals are used for production of recombinant proteins for scientific, pharmaceutical, and agricultural purposes. The ability of transgenic animals to produce biologically active recombinant proteins in an efficient and economic manner was demonstrated a long time ago and has attracted substantial attention and investments. Human interferon-c (hIFN-c) is a key cytokine endowed with multiple biological activities such as antiviral, antibacterial, antiparasitic, antiproliferative, and immunomodulatory activity (Smith et al. 1990; Tsanev and Ivanov 2001). Human IFN-c is a glycoprotein naturally secreted by activated T lymphocytes and monocytes (Rinderknecht et al. 1984). It is a product of a single gene encoding a protein of 143 amino acids with a molecular mass of 20–25 kDa. The recombinant


Fertility and Sterility | 2008

Presence and subcellular localizations of surfactant proteins A and D in human spermatozoa

Orhan Kankavi; Ayhan Ata; Ciler Celik-Ozenci; Leyla Sati; Mehmet Akif Ciftcioglu; Ramazan Demir; Mehmet Baykara

OBJECTIVE To investigate the presence of surfactant protein-A (SP-A); molecular weight 34 kDa and surfactant protein-D (SP-D); and molecular weight 43 kDa in human spermatozoa. DESIGN Prospective, research study. SETTING Two universities in Turkey. PATIENT(S) Semen specimens (n = 10) were obtained from normozoospermic donors. INTERVENTION(S) Human sperm were exposed to an anti-human SP-A polyclonal antibody, and monoclonal antibody, to human SP-D protein. MAIN OUTCOME MEASURE(S) Presence of SP-A and SP-D proteins in human beings. RESULT(S) Indirect immunofluorescence assays of human sperm indicated the presence of SP-A in the mid piece, the tail, and sometimes at the equatorial region of spermatozoa. A brilliant green light detected SP-D in the tails and acrosome of some sperm. The anti-SP-A antibody detected a single band corresponding to the molecular weight values of 34 kDa in spermatozoa, whereas no band was observed in the negative control. The anti-SP-D antibody showed the expected band at 43 kDa in spermatozoa. CONCLUSION(S) This is the first report and a novel finding of the presence of surfactant glycoproteins on human spermatozoa.


Renal Failure | 2014

Evidence of surfactant protein A and D expression decrement and their localizations in human prostate adenocarcinomas

Orhan Kankavi; Mehmet Baykara; Meryem İlkay Eren Karanis; Cumhur İbrahim Başsorgun; Hale Ergin; Mehmet Akif Ciftcioglu

Abstract Aim: Surfactant proteins (SP-A and SP-D) were originally described in the lung; however, they are also present in the prostate. Purpose of this study, therefore, was to determine how surfactant proteins are altered in prostate adenocarcinomas (PCa) and find out any connection exists between their expressions and their staining patterns, prostate-specific antigen (PSA) values, Gleason score, age, tumor volume and tumor, node, metastases (TNM) clinical stage. Methods: Thirty-five tissue samples were obtained during radical prostatectomy. All specimens were classified to three groups based on the Gleason score <7, 7 and Gleason score >7. Surfactant proteins’ expressions were tested by immunohistochemical and Western blotting methods. Results: Immunoreactivity was detected in the cytoplasm from both basal cells and secretory epithelial cells in malignant and non-malignant areas. About 80% of the malignant basal cells were characterized as either weak or strong while non-malignant epithelial cells demonstrated strong immunoreactivity for SP-A. Also malignant (81.8%) and non-malignant cells (90.6%) were characterized as either weak or strong for SP-D. Decrement of SP-A and SP-D immunostaining tended to associate with an increasing Gleason score (p > 0.05, p < 0.05), tumor volume (p < 0.05, p > 0.05) and age (p > 0.05, p > 0.05). There was a strong positive correlation between Gleason score and tumor volume (p < 0.01). Also, either none or weak SP-A and SP-D immunoreactivity was observed specimens with Gleason score 7 or higher. SP-A and SP-D reacted with 34 kDa (SP-A) and 43 kDa (SP-D) immunoreactive single bands were decreased in tumor tissues. Conclusions: The development of prostate cancer may be related to decreased level of surfactant protein A and D.


Journal of Experimental Zoology | 2008

Determination of the expression of fish antifreeze protein (AFP) in 7th generation transgenic mice tissues and serum.

Haydar Bagis; Arzu Tas; Orhan Kankavi

In this study, the presence of antifreeze protein (AFP) gene expression through successive generations in transgenic mice carrying the chimeric gene construct of the coding sequence for the AFP protein from ocean pout was investigated. AFP transgenic hemizygote mice were used for AFP gene expression. AFP genome expressions in transgenic mice were analyzed by Western blotting, and tissue location of AFP protein was shown by immunohistochemical and immunofluorescence techniques. Seventh transgenic mice from the established founders demonstrated the expression of AFP in organs such as the skin, oviduct, lung, kidney and liver tissues and serum except for the heart. Our results demonstrate successful expression of AFP gene products in several tissues and serum of transgenic mice, the association of in vivo expressed AFP protein, for the first time. These results indicate that the coding sequence for the AFP protein gene (ocean pout type III AFP gene) could be integrated and stably transcribed and expressed in the 7th generation of transgenic mice. In conclusion transgenic mouse lines would be a good model for the cryostudy of AFP and for the determination of AFP roles in several organs and tissues.


Animal Reproduction Science | 2007

Surfactant proteins A and D in the genital tract of mares

Orhan Kankavi; Ayhan Ata; Orsan Gungor


Theriogenology | 2006

Surfactant protein A and D in the reproductive tract of stallion

Orhan Kankavi; Ayhan Ata; M. Akif Ciftcioglu


Archives of Dermatological Research | 2008

Surfactant proteins in inflammatory skin diseases: controlled study

Ayse Akman; Orhan Kankavi; Mehmet Akif Ciftcioglu; Erkan Alpsoy


Archive | 2014

Epididimal ve Dondurulmuş Çözülmüş Holstain Boğa Spermasinda Farkli Boyama Yöntemlerinin Karşilaştirilmasi Comparision of Different Staining Methods on Epididymal and Frozen Thawed Sperm from Holstein Bulls

Ayhan Ata; Muhammed Enes; Orhan Kankavi; Mehmet Akif


Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi | 2014

Epididimal ve Dondurulmuş Çözülmüş Holstain Boğa Spermasında Farklı Boyama Yöntemlerinin Karşılaştırılması

Ayhan Ata; Muhammed Enes İnanç; Orhan Kankavi; Özlem Yıldız Gülay; Mehmet Şükrü Gülay


Current Opinion in Biotechnology | 2011

Determination of human interferon-gamma in the transgenic mice milk by modified Kynurenine bioassay test

Haydar Bagis; Digdem Aktoprakligil; Cagatay Günes; Tolga Akkoc; Gaye Cetinkaya; Orhan Kankavi; Ali Cihan Taskin; Sezen Arat; Korhan Arslan; Munis Dundar; Vania L. Tsoncheva; Ivan Ivanov

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Ayhan Ata

Mehmet Akif Ersoy University

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Sezen Arat

Namik Kemal University

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