Osman Fatih Arpağ

Elution of monomer from different bulk fill dental composite resins

OBJECTIVE The purpose of this study was to evaluate the elution of Bis-GMA, TEGDMA, HEMA, and Bis-EMA monomers from six bulk fill composite resins over four different time periods, using HPLC. METHODS Six different composite resin materials were used in the present study: Tetric Evo Ceram Bulk Fill (Ivoclar Vivadent, Amherst, NY), X-tra Fill (VOCO, Cuxhaven, Germany), Sonic Fill (Kerr, Orange, CA, USA), Filtek Bulk Fill (3M ESPE Dental Product, St. Paul, MN), SDR (Dentsply, Konstanz, Germany), EQUIA (GC America INC, Alsip, IL). The samples (4mm thickness, 5mm diameter) were prepared and polymerized for 20s with a light emitted diode unit. After fabrication, each sample was immediately immersed in 75wt% ethanol/water solution used as extraction fluid and stored in the amber colored bottles at room temperature. Ethanol/water samples were taken (0.5mL) at predefined time intervals:10m (T1), 1h (T2), 24h (T3) and 30 days (T4). These samples were analyzed by HPLC. The obtained data were analyzed with one-way ANOVA and Tukey HSD at significance level of p<0.05. RESULTS Amount of eluted Bis-EMA and Bis-GMA from Tetric Evo Ceram Bulk Fill and amount of eluted TEGDMA and HEMA from X-tra Fill higher than others composites (p<0.05). SIGNIFICANCE Residual monomers were eluted from bulk fill composite resins in all time periods and the amount of eluted monomers was increased with time.

Comparison of osteoconductive properties of three different β-tricalcium phosphate graft materials: a pilot histomorphometric study in a pig model.

AIMS The aim of this study was to compare the de novo bone formation ability and osteoconductive effects of three different β-tricalcium phosphate (β-TCP) graft materials. The micro-architectural parameters of the newly formed bone tissues were also compared among the different graft materials. MATERIAL AND METHODS Eight male Swiss domestic pigs were used in the study. Five bony defects were made with a trephine bur. Three of the defects were filled with Cerasorb®, Kasios® and Poresorb®. The fourth defect was filled with an autogenous bone graft. The last defect remained empty. All subjects were sacrificed after 8 weeks. RESULTS When compared to a negative control group, significant healing was observed in all the groups except the Cerasorb group. The osteoconductivity of the Poresorb group was better than that of the other groups (p < 0.05). The difference in the osteoconductivity of the Kasios and Cerasorb groups was statistically significant (p < 0.05). Comparison of the micro-architectural properties of newly formed bone tissues retrieved from the defects showed that those filled with Poresorb were the best. CONCLUSION β-TCP materials show different results in terms of the volume and characteristics of new bone formation, although they have a similar chemical structure.

Effects of caffeic acid phenethyl ester on palatal mucosal defects and tooth extraction sockets.

Aim The purpose of this study was to evaluate the effects of caffeic acid phenethyl ester (CAPE) on palatal mucosal defects and tooth extraction sockets in an experimental model. Materials and methods Forty-two male Sprague-Dawley rats with a mean age of 7 weeks and weighing 280–490 g were used in this study. The rats were randomly divided into two groups: group A (the control group, n=21) and group B (the experimental group, n=21). Under anesthesia with ketamine (8 mg/100 g, intraperitoneally), palatal mucosal defects were created and tooth extraction was performed in the rats in groups A and B. Group A received no treatment, whereas group B received CAPE. CAPE was injected daily (10 μmol/kg, intraperitoneally). The rats were killed on days 7, 14, and 30 after the procedures. Palatal mucosa healing and changes in bone tissue and fibrous tissue were evaluated histopathologically. Result Pairwise comparisons showed no statistically significant difference between days 7 and 14 in either group (P>0.05). At day 30, bone healing was significantly better in group B (CAPE) than in group A (control) (P<0.05). Fibrinogen levels at day 30 were significantly higher in group A (control) than in group B (CAPE) (P<0.05). Pairwise comparisons showed no statistically significant difference in palatal mucosa healing levels between days 7 and 14 in both groups (P>0.05). Conclusion In conclusion, the findings of this study suggest that CAPE can significantly improve tooth socket healing.

Angioleiomyoma of the gingiva: a report of two cases

Leiomyoma is a type of benign smooth muscle neoplasm that is a common neoplasm of the uterus and gastrointestinal tract but rarely affects the head and neck region and is especially unlikely to affect the oral cavity. The diagnosis of leiomyoma is mainly determined by histopathological studies due to variation in its clinical appearance and symptoms. In the present paper we report two rare cases of gingival angioleiomyoma in the posterior maxilla and mandible. After total excision, hematoxylin-eosin and smooth muscle actin staining confirmed the diagnosis of angioleimyoma.

To what extent does hyaluronic acid affect healing of xenografts? A histomorphometric study in a rabbit model

ABSTRACT Among the many graft materials that have been used for the treatment of bone defects in oral and maxillofacial regions is xenograft. To improve osteoconductive effects of xenografts, they have been combined with various biocompatible materials, such as hyaluronic acid and bone morphogenetic protein. Objective To determine bone-healing capacity of high molecular weight hyaluronic acid (HA) combined with xenograft in rabbit calvarial bone defects. Material and methods Ten adult male New Zealand rabbits (mean weight 3 kg) were included in the study. Three 6-mm-diameter bicortical cranial defects were created on calvarial bone of all rabbits. These defects were filled as follows: a) xenograft; b) HA+xenograft; c) autograft. One month after the first operation, rabbits were sacrificed. Specimens were evaluated histomorphometrically. Results Considering multiple comparisons, differences regarding new bone were statistically significant between all groups (p<0.05). The volume of residual graft was significantly decreased in HA group compared to xenograft group (p=0.035). Marrow space, trabecular thickness (TbTh), trabecular width (TbWi), trabecular separation (TbSp), and number of node: number of terminus (NNd:NTm) in the autograft group were significantly better than xenograft and HA groups (p<0.05). However, regarding marrow space, TbTh, TbWi, TbSp, and NNd:NTm values, xenograft and HA groups showed similar results and the difference were not significant (p>0.05). Conclusion These results support that high molecular weight hyaluronic acid could contribute to the healing of xenograft by improving the percentage of new bone formation and reducing the percentage of residual graft. However, HA did not significantly affect the quality of newly formed bone assessed by microarchitectural parameters.

Rotational autotransplantation of mandibular alveolar ridge.

l r c d f he rehabilitation of edentulous atrophic mandibles with ental implants is often threatened where there has been oss of horizontal alveolar bone. Techniques to augment the one include guided bony regeneration, distraction osteoenesis, ridge splitting osteotomy, onlay grafting with block rafts (autologous or xenograft), or combinations of these.1 he mandibular symphysis, ramus, maxillary tuberosity, iliac rest, tibia, calvaria, and costae are all sources of autologous one. These grafts remain the gold standard,2 but morbidty at the donor site and second operations can be serious isadvantages. We describe a technique in which we used a modied autologous block graft to reconstruct the horizontally esorbed anterior mandible to provide adequate height. After crestal incision, we raised the mucoperiosteal flap at the nterforaminal area, and made a horizontal bicortical cut at east 6 mm apically from the alveolar crest. We chose piezourgery equipment for the osteotomy as it provides good anipulation with its variably-angled handpieces, leaves fine ines with minimal loss of bone, and causes negligible damge to the lingual soft tissues.3 We removed the bone block Fig. 1) and rotated it 180◦ in the coronal plane (the left side f the graft should turn to the right side of the mandible, and he upper border of the graft should turn to the lower borer) (Fig. 2). We used a titanium miniscrew to fix the graft o the buccal surface of the anterior mandible just below the

Effects of Hypericum perforatum on the healing of xenografts: a histomorphometric study in rabbits

The aim of this study was to investigate effects of the Hypericum perforatum (St Johns Wort) on bone healing in rabbit calvarium. Ten male New Zealand rabbits each had three bicortical defects made in the calvarial bones, which were filled with xenograft, xenograft+H perforatum oil extract, and autogenous graft. Four weeks postoperatively all rabbits were killed and the bony defects examined histomorphometrically. Tissue compartments including new bone (p<0.001), marrow space (p<0.001), and residual bone grafts (p=0.014) differed significantly among groups. The volume of residual graft was significantly decreased in the xenograft/H perforatum group compared with those with xenografts alone (p=0.0147). The differences in microarchitectural variables of de novo bone formation were also significant (trabecular thickness (p<0.001), trabecular width (p<0.001), trabecular separation (p=0.001). There were no significant differences in node:terminus ratio between the xenograft/H perforatum group and the other two groups. However, the difference in node:terminus ratio between the autogenous graft and xenograft group was significant (p=0.001) Oil extracts of H perforatum improved bony healing in defects filled with bovine-derived xenografts.

Evaluation of patient with Holt-Oram syndrome in terms of oral and maxillofacial findings

The Holt-Oram syndrome (HOS) is a genetic disorder with autosomal dominant inheritance associated with anomalies in upper extremities and heart and affects one out of every 100,000 live births. Maxillofacial development is also affected by these skeletal abnormalities. Although there are many studies about the HOS in the literature, the data about the development of oral and maxillofacial development are very few. In this study, evaluation of a child with the HOS is made in terms of dental and gingival health, oral and maxillofacial formation, and cephalometric analysis measurements are made for the 1 st time in the literature and identified findings are discussed in company with the literature.

The effects of idiopathic hypoparathyroidism on dental development

Hypoparathyroidism cases without etiology diagnosed in the first 10 years of life are called as idiopathic hypoparathyroidism (IHP). IHP is a rare endocrinological disorder among hypoparathyroidism cases, and the anomalies accompanying it are infrequently observed. Ca ++ metabolism disorders occurring during the formation of tooth germs may cause enamel defects in teeth, enamel hypoplasia, enlarged pulp chambers, short roots, late eruptions, and missing teeth. The case presented in this article is a good example in terms of demonstrating how tooth damage ends with the commencement of treatment. Within this article, the results of a 9-year-old male IHP patient are discussed based on the literature. The said patient could not be diagnosed until the age of 18 months so developed enamel hypoplasia as well as enamel defects in this period due to abnormal mineralization, and no problems were observed in his dental tissue that formed after the ionic equilibrium was ensured following the diagnosis.

Maksiller Lateral Diş ile Süpernümerer Diş Füzyonu ve Tedavisi: Bir Olgu Sunumu

Fusion is a dental anomaly which causes problems such as aesthetics, periodontal, orthodontic disease. The purpose of this study is to provide aesthetics by extraction of supernumerary fused tooth with lateral tooth that is causing lack of space. It is a sharing case that aestetic and function were ensured by formed lateral tooth. 10 years old boy patient admitted to our clinic with the complaint of his tooth curvature. According to history of the patient, there is no systemic disease of the patient. No problems were observed in the extraoral diagnosis. In the intraoral diagnosis, presence of supernumerary teeth and fused with lateral tooth was observed. In the radiological diagnosis, roots are discrete. Another pathology were not encountered in the mouth. It was planned that the separation of the fused teeth by cut by cutters from junction line and distopalatinal supernumerary tooth was extracted. Perforation was observed on the lateral tooth and treated with CVEK amputation method. Treatment was completed with composite restoration. Controls of patient performed at 12 months. Treatment provided aesthetics by extraction of supernumerary fused tooth with lateral tooth that is causing lack of space so malalignment of teeth has disappeared. Perforation of lateral tooth was treated with amputation method