Özer Kolsarici

Ankara Koşullarında Hibrit Çerezlik Ayçiçeği (Helianthus annuus L.) Genotipinde Farklı Sıra Üzeri Aralıkları ve Azot Dozlarının Verim ve Verim Ögelerine Etkisi

Bu arastirma, Ankara kosullarinda uygulanan farkli sira uzeri mesafe (20 cm, 30 cm ve 40 cm) ve azot dozlarinin (0, 4, 8 ve 12 kg da-1) 03M142 hibrit cerezlik aycicegi genotipinde verim ve verim ogeleri uzerine etkilerini belirlemek amaciyla 2007 ve 2008 yillarinda yurutulmustur. Deneme, tesaduf bloklarinda bolunmus parseller deneme desenine gore uc tekrarlamali olarak kurulmustur. Arastirmada, bitki boyu, tabla capi, bin tane agirligi, kabuk orani, bitkide tane verimi, hasat indeksi, dekara tane verimi, yag orani ve protein orani ozellikleri incelenmistir. Arastirma sonuclarina gore, en uzun bitki boyu 157,2 cm ile 12 kg N da-1 dozunda, en yuksek dekara tane verimi ise ikinci yilda 20 cm sira uzeri aralikta 12 kg N da-1 uygulamasinda 410,3 kg da-1 olarak elde edilmistir. Sonuc olarak, sira uzeri araligin daralmasi ve bununla beraber azot dozlarinin da artmasi verim artisina sebep olmustur.

Establishment of efficient regeneration protocol for three rapeseed cultivars

Economically, rapeseed is one of the most important crops in the world. Over the past decades, rapeseed research has been focused on improving biotechnological methods to facilitate breeding. The effectiveness of these methods depends on efficient tissue culture techniques. The aim of the present study was to establish an efficient protocol for regeneration of rapeseed. It was conducted in three stages. The first stage was to determine the effect of different concentrations (10%, 20%, 30% and 40%) of sodium hypochlorite solutions on seedling growth parameters. In the second stage, the effects of different growth media (Murashige and Skoog, MS, and Gamborg) and plant growth regulators (6-benzylaminopurine, 1-naphthaleneacetic acid and thidiazuron) at different concentrations on the regeneration capacity of stem explants of three rapeseed cultivars were investigated. In the last stage, the shoots of ‘Spok’ were cultured for three weeks on MS medium containing 1.5, 3 and 4.5 mg L−1 of indol butyric acid for rooting. The best results in germination, seedling growth and root length were obtained by using 10% disinfectant concentration for 25 minutes. The highest values for shoot regeneration were obtained from the stem explant cultured on MS medium containing 3 mg L−1 BAP and 0.2 mg L−1 NAA. It was found that MS containing 1.5 mg L−1 IBA was the most efficient medium for root formation.