P. Chowdappa

Phytophthora boehmeriae Revealed as the Dominant Pathogen Responsible for Severe Foliar Blight of Capsicum annuum in South India

Prior to 2011, foliar blight was not reported as a serious threat to hot pepper cultivation in India. During the June-to-January cropping season of 2011 and 2012, severe foliar blight epidemics were observed in Karnataka and Tamil Nadu states of India. In all, 52 Phytophthora isolates, recovered from blight-affected leaf tissues of hot pepper from different localities in Karnataka and Tamil Nadu states between 2011 and 2012, were identified: 43 isolates as P. boehmeriae and 9 isolates as P. capsici, based on morphology, a similarity search of internal transcribed spacer sequences at GenBank, polymerase chain reaction (PCR) restriction fragment length polymorphism patterns, and species-specific PCR using PC1/PC2 and PB1/PB2 primer pairs. The isolates were further assessed for metalaxyl sensitivity and aggressiveness on hot pepper. All isolates of P. boehmeriae were metalaxyl sensitive while P. capsici isolates were intermediate in sensitivity. P. boehmeriae isolates were highly aggressive and produced significantly (P < 0.01) larger lesion than those of P. capsici isolates. Thus, emergence of P. boehmeriae was responsible for severe leaf blight epidemics on hot pepper in South India, although it is not serious pathogen on any crop in any part of the world. These results have epidemiological and management implications for the production of hot pepper in India.

Population Structure of Phytophthora nicotianae Reveals Host-Specific Lineages on Brinjal, Ridge Gourd, and Tomato in South India

Severe outbreaks of Phytophthora fruit rot on brinjal, ridge gourd, and tomato have been observed since 2011 in Andhra Pradesh, Karnataka, Telangana, and Tamil Nadu states of India. Therefore, 76 Phytophthora nicotianae isolates, recovered from brinjal (17), ridge gourd (40), and tomato (19) from different localities in these states during the June to December cropping season of 2012 and 2013, were characterized based on phenotypic and genotypic analyses and aggressiveness on brinjal, tomato, and ridge gourd. All brinjal and ridge gourd isolates were A2, while tomato isolates were both A1 (13) and A2 (6). All isolates were metalaxyl sensitive. In addition, isolates were genotyped for three mitochondrial (ribosomal protein L5-small subunit ribosomal RNA [rpl5-rns], small subunit ribosomal RNA-cytochrome c oxidase subunit 2 [rns-cox2], and cox2+spacer) and three nuclear loci (hypothetical protein [hyp], scp-like extracellular protein [scp], and beta-tubulin [β-tub]). All regions were polymorphic but nuclear regions were more variable than mitochondrial regions. The network analysis of genotypes using the combined dataset of three nuclear regions revealed a host-specific association. However, the network generated using mitochondrial regions limited such host-specific groupings only to brinjal isolates. P. nicotianae isolates were highly aggressive and produced significantly (P ≤ 0.01) larger lesions on their respective host of origin than on other hosts. The results indicate significant genetic variation in the population of P. nicotianae, leading to identification of host-specific lineages responsible for severe outbreaks on brinjal, ridge gourd, and tomato.