Pang Daiwen

Microcalorimetric investigation of the effect of La3+ on mitochondria isolated from avian chicken liver tissue cells

A microcalorimetric technique was used to evaluate the influence of La3+ on mitochondria isolated from the liver tissue of Avian chicken. By means of LKB-2277 bioactivity monitor, ampoule method at 37°C, we obtained the thermogenic curves of the metabolism of mitochondria. After isolation from the chicken liver tissue, mitochondria still have metabolic activity and can live for a long time depending on the stored nutrients. In order to analyze the results, the maximum power (Pm) and the decline rate constants (kd) were obtained. The addition of La3+ results in an increase of the maximum heat production and decline rate constants. Furthermore, values of Pm and kd are linked to the concentration of La3+. According to the thermogenic curves under different conditions, it is clear that metabolic mechanism of mitochondria has been changed with the addition of La3+.

Construction of CdSe/ZnS quantum dot microarray in a microfluidic chip

Microarray technology has been proved to be greatly helpful for biomedical and biological diagnosis. And the evaluation of its biological applications lies in the detection sensitivity, which requires high intensity and stability of the signal. Recently, several nanomaterials, especially semiconductor nanomaterials, due to their excellent fluorescence properties, have been widely used to construct microarrays for biosensors. Here, we presented an approach for constructing CdSe/ZnS quantum dot (QD) microarray in microfluidic channels on a glass slide by photolithography. The conditions for immobilizing stable and uniform QD microarray on the glass slide were optimized. Several types of QD microarrays with different emission wavelengths and modified groups were constructed using silanization and lithography technology. Based on the fluorescence quenching effect of Cu2+ on QDs, the microfluidic chip with QD microarray was applied for the determination of Cu2+. 1 nmol/L Cu2+ could be detected by this method.

Live cell synthesis of inorganic nanomaterials

纳米材料由于优异的光学、电学、磁学等性质, 而被广泛应用于生物检测、示踪、光电转换及光热治疗等研究中. 通过调节纳米材料的组成、结构、尺寸及形貌等, 可以有效地调控纳米材料的性能, 已发展的化学合成方法在实现纳米材料性能的调控方面取得了很大进展. 随着化学、材料学及生物学等多学科的交叉, 人们开始探索在纳米材料的合成中利用生物体系的调控网络来进行合成控制, 以期更精确地控制纳米材料的结构及其性质, 从而出现了纳米材料的活细胞合成方法. 由于活细胞内的反应均受到精确调控, 如果能被科学地利用, 将可望实现纳米材料组成、结构、尺寸、形貌和性质的控制.

Assembling zwitterionic, water-soluble porphyrin and its biological studies

A kind of zwitterionic water-soluble porphyrin 5-(N-trimethyl-4-aminiumphenyl)-10, 15, 20-tri (4-sulfonatephenyl) porphyrin trisammonium salt, has been synthesized. Its biological studies, interactions with DNA and bacteria cells, were investigated. The damage to the cell wall of bacteriaE. coli were observed firstly by AFM (Atomic Force Microscope).

Amperometric detection of histamine and amino acid neurotransmitters in capillary electrophoresis with copper microelectrodes

Copper microelectrodes were used in microvoltammetry to detect histamine and several amino acid neurotransmitters. High sensitivity was obtained through the use of copper microelectrodes, and detection limits of 50 nmol/L and 90 nmol/L were obtained for histamine and histidine, respectively. Furthermore, histamine and several amino acids neurotransmitters were firstly separated and detected by capillary electrophoresis with amperometric detection on copper microelectrodes. Mass limits of 490 amol and 440 amol were achieved for histamine and histidines, respectively, by using this mothod.