Zhang Zhiling

Construction of CdSe/ZnS quantum dot microarray in a microfluidic chip

Microarray technology has been proved to be greatly helpful for biomedical and biological diagnosis. And the evaluation of its biological applications lies in the detection sensitivity, which requires high intensity and stability of the signal. Recently, several nanomaterials, especially semiconductor nanomaterials, due to their excellent fluorescence properties, have been widely used to construct microarrays for biosensors. Here, we presented an approach for constructing CdSe/ZnS quantum dot (QD) microarray in microfluidic channels on a glass slide by photolithography. The conditions for immobilizing stable and uniform QD microarray on the glass slide were optimized. Several types of QD microarrays with different emission wavelengths and modified groups were constructed using silanization and lithography technology. Based on the fluorescence quenching effect of Cu2+ on QDs, the microfluidic chip with QD microarray was applied for the determination of Cu2+. 1 nmol/L Cu2+ could be detected by this method.

Live cell synthesis of inorganic nanomaterials

纳米材料由于优异的光学、电学、磁学等性质, 而被广泛应用于生物检测、示踪、光电转换及光热治疗等研究中. 通过调节纳米材料的组成、结构、尺寸及形貌等, 可以有效地调控纳米材料的性能, 已发展的化学合成方法在实现纳米材料性能的调控方面取得了很大进展. 随着化学、材料学及生物学等多学科的交叉, 人们开始探索在纳米材料的合成中利用生物体系的调控网络来进行合成控制, 以期更精确地控制纳米材料的结构及其性质, 从而出现了纳米材料的活细胞合成方法. 由于活细胞内的反应均受到精确调控, 如果能被科学地利用, 将可望实现纳米材料组成、结构、尺寸、形貌和性质的控制.