Patrícia Oliveira Santos

Chemical composition and antimicrobial activity of the essential oil of Hyptis pectinata (l.) Poit.

Essential oil was extracted from leaves of Hyptis pectinata using hydrodistillation, and its composition determined using GC-FID and GC-MS. Chemical analysis showed that there was a predominance of sesquiterpenes, of which β-caryophyllene (18.34%), caryophyllene oxide (18.00%) and calamusenone (24.68%) were measured for the first time in the genus Hyptis. Twenty-one compounds were identified, and calamusenone was isolated using preparative thin layer chromatography with a silica gel plate (60 PF254). The minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) were determined for various pathogenic microorganisms. H. pectinata oil was most effective against Gram (+) bacteria and yeasts.

Multilocus enzyme electrophoresis analysis and exoenzymatic activity of Candida albicans strains isolated from women with vaginal candidiasis.

Twenty‐eight Candida albicans strains obtained from women with vaginal candidiasis were tested for phospholipase and proteinase production and clustered by multilocus enzyme electrophoresis (MLEE). The proteolytic and phospholipidic activity were considered moderate (0.56 ± 0.12 mm and 0.53 ± 0.09 mm, respectively) for all isolates. The isoenzymes malate dehydrogenase (MDH) and sorbitol dehydrogenase (SDH) showed strong intra‐specific discriminatory power. The numerical and genetic interpretation of the bands produced by the isoenzymes tested presented similar discriminatory power. The genetic diversity of the isolates was measured by allelic and genic frequency, perceptual index of polymorphic loci (P = 87.5%), average number of alleles per locus, average number of alleles per polymorphic locus, average heterozygosity observed and average heterozygosity expected. We verified that three isoenzymatic loci (Adh, Gdh and Sdh‐2) were not in Hardy–Weinberg equilibrium. A dendrogram constructed based on the genetic distance matrix of Nei showed seven clusters; 57.15% (16) of the isolates were considered highly related or indistinguishable, and 42.85% were considered moderately related or unrelated. We did not find a relationship between the clusters and the exoenzymes production.

O ensino de Ciências Naturais e cidadania sob a ótica de professores inseridos no programa de aceleração de aprendizagem da EJA - Educação de Jovens e Adultos

O presente estudo teve como objetivo conhecer as concepcoes dos professores da EJA - Educacao de Jovens e Adultos - no aspecto referente a interface ensino Ciencias Naturais e cidadania. Serviu ainda para identificar as principais dificuldades encontradas na pratica desses docentes para a formacao de alunos-cidadaos, assim como revelar a atual situacao do ensino de Ciencias Naturais no referido programa. Para tanto, foram entrevistados 19 professores que lecionam a disciplina Ciencias Naturais nas turmas de 5a a 8a serie, inseridas no Programa de Aceleracao de Aprendizagem da EJA, em escolas publicas municipais de Aracaju, SE. Como instrumentos de coleta de dados, utilizou-se a tecnica de entrevista semi-estruturada e a analise de documentos, com enfoque qualitativo.

NEW ALKYL PHENOLS AND FATTY ACID PROFILE FROM OILS OF PULPED Spondias mombin L. SEED WASTES

Larissa C. de Rezendea,c, Patricia de A. Santosa, Valéria B. Riattoa, Jorge M. Davida,* and Juceni P. Davidb Instituto de Química, Universidade Federal da Bahia, 40170-290 Salvador – BA, Brasil Faculdade de Farmácia, Universidade Federal da Bahia, 40170-290 Salvador – BA, Brasil Departamento de Ciências Exatas e Naturais, Universidade Estadual do Sudoeste da Bahia, Praça Primavera, 40, 45700-000 Itapetinga – BA, Brasil

Inhibition of Drug-Sensitive and Drug-Resistant Mycobacterium Tuberculosis Strains by Essential Oil from Croton Argyrophylloides Mull. Arg.

Objetcitve: To assess in vitro effects of essential oil extracted from Croton argyrophylloides against clinical and standard strains of Mycobacterium tuberculosis , comparing its resistance and sensitivity profiles to drugs commonly used in therapy. Method: In the present study, chemical composition and antimycobacterial activity of essential oils extracted from aerial parts of Croton argyrophylloides collected in Curituba district, Sergipe, Brazil were analysed. The oil was studied by GC and GC-MS and its antimicobacterial activity (MIC) was tested against 49 clinical and standard covers H 37 RV using the REMA method. To access sensitivity to isoniazid, rifampicin, ethambutol and streptomycin it was used proportions indirect test method. Results: Twenty-seven chemicals were identified, totalizing 95.98% of the composition of essential oil. The oil presented good antibacterial activity (MIC = 97 to 195 g / ml) against strains of Mycobacterium tuberculosis H37Rv and pattern one. Conclusion: On pharmacological activities of these species confirmed in vitro scientific support for its use in traditional herbal preparations.