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Dive into the research topics where Paul C. DeRose is active.

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Featured researches published by Paul C. DeRose.


Pure and Applied Chemistry | 2010

Fluorescence standards: Classification, terminology, and recommendations on their selection, use, and production (IUPAC Technical Report)

Ute Resch-Genger; Paul C. DeRose

Chromophore-based fluorescence standards for the characterization of photo-luminescence measuring systems and the determination of relevant fluorometric quantities are classified according to their scope and area of application. General and type-specific requirements for suitable standards are derived for each class of standards. Metrological requirements linked to the realization of comparable measurements are addressed and recommendations on selecting, using, and developing fluorescence standards are given.


Analytical Chemistry | 2010

Recommendations for Fluorescence Instrument Qualification: The New ASTM Standard Guide

Paul C. DeRose; Ute Resch-Genger

Aimed at improving quality assurance and quantitation for modern fluorescence techniques, ASTM International (ASTM) is about to release a Standard Guide for Fluorescence, reviewed here. The guides main focus is on steady state fluorometry, for which available standards and instrument characterization procedures are discussed along with their purpose, suitability, and general instructions for use. These include the most relevant instrument properties needing qualification, such as linearity and spectral responsivity of the detection system, spectral irradiance reaching the sample, wavelength accuracy, sensitivity or limit of detection for an analyte, and day-to-day performance verification. With proper consideration of method-inherent requirements and limitations, many of these procedures and standards can be adapted to other fluorescence techniques. In addition, procedures for the determination of other relevant fluorometric quantities including fluorescence quantum yields and fluorescence lifetimes are briefly introduced. The guide is a clear and concise reference geared for users of fluorescence instrumentation at all levels of experience and is intended to aid in the ongoing standardization of fluorescence measurements.


Pure and Applied Chemistry | 2012

Characterization of photoluminescence measuring systems (IUPAC Technical Report)

Ute Resch-Genger; Paul C. DeRose

Procedures for the characterization of photoluminescence measuring systems are discussed, focusing on spectrofluorometers and fit-for-purpose methods including suitable standards. The aim here is to increase the awareness for the importance of a reliable instrument characterization and to improve the reliability and comparability of measurements of photoluminescence.


Analytical Chemistry | 2012

State-of-the Art Comparability of Corrected Emission Spectra.1. Spectral Correction with Physical Transfer Standards and Spectral Fluorescence Standards by Expert Laboratories

Ute Resch-Genger; Wolfram Bremser; Dietmar Pfeifer; Monika Spieles; Angelika Hoffmann; Paul C. DeRose; Joanne C. Zwinkels; François Gauthier; Bernd Ebert; R. Dieter Taubert; Christian Monte; Jan Voigt; J. Hollandt; Rainer Macdonald

The development of fluorescence applications in the life and material sciences has proceeded largely without sufficient concern for the measurement uncertainties related to the characterization of fluorescence instruments. In this first part of a two-part series on the state-of-the-art comparability of corrected emission spectra, four National Metrology Institutes active in high-precision steady-state fluorometry performed a first comparison of fluorescence measurement capabilities by evaluating physical transfer standard (PTS)-based and reference material (RM)-based calibration methods. To identify achievable comparability and sources of error in instrument calibration, the emission spectra of three test dyes in the wavelength region from 300 to 770 nm were corrected and compared using both calibration methods. The results, obtained for typical spectrofluorometric (0°/90° transmitting) and colorimetric (45°/0° front-face) measurement geometries, demonstrated a comparability of corrected emission spectra within a relative standard uncertainty of 4.2% for PTS- and 2.4% for RM-based spectral correction when measurements and calibrations were performed under identical conditions. Moreover, the emission spectra of RMs F001 to F005, certified by BAM, Federal Institute for Materials Research and Testing, were confirmed. These RMs were subsequently used for the assessment of the comparability of RM-based corrected emission spectra of field laboratories using common commercial spectrofluorometers and routine measurement conditions in part 2 of this series (subsequent paper in this issue).


PLOS ONE | 2013

Beetroot-Pigment-Derived Colorimetric Sensor for Detection of Calcium Dipicolinate in Bacterial Spores

Letícia Christina Pires Gonçalves; Sandra M. Da Silva; Paul C. DeRose; Rômulo A. Ando; Erick L. Bastos

In this proof-of-concept study, we describe the use of the main red beet pigment betanin for the quantification of calcium dipicolinate in bacterial spores, including Bacillus anthracis. In the presence of europium(III) ions, betanin is converted to a water-soluble, non-luminescent orange 1∶1 complex with a stability constant of 1.4×105 L mol–1. The addition of calcium dipicolinate, largely found in bacterial spores, changes the color of the aqueous solution of [Eu(Bn)+] from orange to magenta. The limit of detection (LOD) of calcium dipicolinate is around 2.0×10–6 mol L–1 and the LOD determined for both spores, B. cereus and B. anthracis, is (1.1±0.3)×106 spores mL–1. This simple, green, fast and low cost colorimetric assay was selective for calcium dipicolinate when compared to several analogous compounds. The importance of this work relies on the potential use of betalains, raw natural pigments, as colorimetric sensors for biological applications.


Cytometry Part A | 2014

An automated protocol for performance benchmarking a widefield fluorescence microscope

Michael Halter; Elianna Bier; Paul C. DeRose; Gregory A. Cooksey; Steven J. Choquette; Anne L. Plant; John T. Elliott

Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other imaging applications, few published methods exist for evaluating and benchmarking the analytical performance of a microscope. Easy‐to‐use benchmarking methods would facilitate the use of fluorescence imaging as a quantitative analytical tool in research applications, and would aid the determination of instrumental method validation for commercial product development applications. We describe and evaluate an automated method to characterize a fluorescence imaging systems performance by benchmarking the detection threshold, saturation, and linear dynamic range to a reference material. The benchmarking procedure is demonstrated using two different materials as the reference material, uranyl‐ion‐doped glass and Schott 475 GG filter glass. Both are suitable candidate reference materials that are homogeneously fluorescent and highly photostable, and the Schott 475 GG filter glass is currently commercially available. In addition to benchmarking the analytical performance, we also demonstrate that the reference materials provide for accurate day to day intensity calibration. Published 2014 Wiley Periodicals Inc.


Analytical Chemistry | 2012

State-of-the Art Comparability of Corrected Emission Spectra. 2. Field Laboratory Assessment of Calibration Performance Using Spectral Fluorescence Standards

Ute Resch-Genger; Wolfram Bremser; Dietmar Pfeifer; Monika Spieles; Angelika Hoffmann; Paul C. DeRose; Joanne C. Zwinkels; François Gauthier; Bernd Ebert; R. Dieter Taubert; Jan Voigt; J. Hollandt; Rainer Macdonald

In the second part of this two-part series on the state-of-the-art comparability of corrected emission spectra, we have extended this assessment to the broader community of fluorescence spectroscopists by involving 12 field laboratories that were randomly selected on the basis of their fluorescence measuring equipment. These laboratories performed a reference material (RM)-based fluorometer calibration with commercially available spectral fluorescence standards following a standard operating procedure that involved routine measurement conditions and the data evaluation software LINKCORR developed and provided by the Federal Institute for Materials Research and Testing (BAM). This instrument-specific emission correction curve was subsequently used for the determination of the corrected emission spectra of three test dyes, X, QS, and Y, revealing an average accuracy of 6.8% for the corrected emission spectra. This compares well with the relative standard uncertainties of 4.2% for physical standard-based spectral corrections demonstrated in the first part of this study (previous paper in this issue) involving an international group of four expert laboratories. The excellent comparability of the measurements of the field laboratories also demonstrates the effectiveness of RM-based correction procedures.


Need for and Metrological Approaches Towards Standardization of Fluorescence Measurements From the View of National Metrology Institutes | 2008

Need for and Metrological Approaches Towards Standardization of Fluorescence Measurements from the View of National Metrology Institutes

Paul C. DeRose; Lili Wang; Adolfas K. Gaigalas; Gary W. Kramer; Ute Resch-Genger; Ulrich Panne

The need for standardization in fluorescence measurements to improve quality assurance and to meet regulatory demands is addressed from the viewpoint of National Metrology Institutes (NMIs). Classes of fluorescence standards are defined, including instrument calibration standards for the determination and correction of instrument bias, application-specific standards based on commonly used fluorescent labels, and instrument validation standards for periodic checks of instrument performance. The need for each class of standard is addressed and on-going efforts by NMIs and others are described. Several certified reference materials (CRMs) that have recently been developed by NMIs are highlighted. These include spectral correction standards, developed independently by both NIST and BAM (Germany), and fluorescence intensity standards for flow cytometry, developed by NIST. In addition, future activities at both institutes are addressed such as the development of day-to-day intensity standards.


Photochemistry and Photobiology | 2007

Photophysical properties of ricin.

Adolfas K. Gaigalas; Kenneth D. Cole; S Bykadi; Lili Wang; Paul C. DeRose

The molar absorption coefficient of ricin in phosphate‐buffered saline (PBS) at 279 nm was measured as (93 900 ± 3300) L mol−1 cm−1. The concentration of ricin was determined using amino acid analysis. The absorption spectrum of ricin was interpreted in terms of 69% contribution from absorption by tryptophan residues and 31% contribution from absorption by tyrosine residues. The total dipole strength of the ricin band at 280 nm was determined to be (147 ± 8) D2 and was consistent with the combined dipole strengths of 10 tryptophan ([11.7 ± 1.0] D2) and 23 tyrosine ([1.4 ± 0.2] D2) residues. The structure of ricin was used to determine the coupling of the tryptophan residues in ricin. The maximum interaction energy was found to be 424 cm−1/ɛ while the average interaction between any two pairs of tryptophan residues was approximately 18 cm−1/ɛ. In this study, ɛ is the dielectric constant inside the protein. The fluorescence from ricin, excited at 280 nm, was dominated by fluorescence from tryptophan residues suggesting the presence of energy transfer from tyrosine to tryptophan residues. The absorbance and fluorescence of ricin increased slightly when ricin was denatured in a high concentration of guanidine. Irreversible thermal unfolding of ricin occurred between 65°C and 70°C.


Analytical and Bioanalytical Chemistry | 2018

The NISTmAb Reference Material 8671 value assignment, homogeneity, and stability

John E. Schiel; Abby Turner; Trina Mouchahoir; Katharina Yandrofski; Srivalli Telikepalli; Jason King; Paul C. DeRose; Dean C. Ripple; Karen W. Phinney

AbstractThe NISTmAb Reference Material (RM) 8671 is intended to be an industry standard monoclonal antibody for pre-competitive harmonization of best practices and designing next generation characterization technologies for identity, quality, and stability testing. It must therefore embody the quality and characteristics of a typical biopharmaceutical product and be available long-term in a stable format with consistent product quality attributes. A stratified sampling and analysis plan using a series of qualified analytical and biophysical methods is described that assures RM 8671 meets these criteria. Results for the first three lots of RM 8671 highlight the consistency of material attributes with respect to size, charge, and identity. RM 8671 was verified to be homogeneous both within and between vialing lots, demonstrating the robustness of the lifecycle management plan. It was analyzed in concert with the in-house primary sample 8670 (PS 8670) to provide a historical link to this seminal material. RM 8671 was verified to be fit for its intended purpose as a technology innovation tool, external system suitability control, and cross-industry harmonization platform. Graphical abstractThe NISTmAb Reference Material (RM) 8671 is intended to be an industry standard monoclonal antibody for pre-competitive harmonization of best practices and designing next generation characterization technologies for identity, quality, and stability testing.

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Ute Resch-Genger

Bundesanstalt für Materialforschung und -prüfung

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Steven J. Choquette

National Institute of Standards and Technology

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Adolfas K. Gaigalas

National Institute of Standards and Technology

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Gary W. Kramer

National Institute of Standards and Technology

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Kenneth D. Cole

National Institute of Standards and Technology

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Lili Wang

National Institute of Standards and Technology

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Anne L. Plant

National Institute of Standards and Technology

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Gregory A. Cooksey

National Institute of Standards and Technology

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