Paul Delamarche

Catecholamines and the Effects of Exercise, Training and Gender

Stress hormones, adrenaline (epinephrine) and noradrenaline (norepinephrine), are responsible for many adaptations both at rest and during exercise. Since their discovery, thousands of studies have focused on these two catecholamines and their importance in many adaptive processes to different stressors such as exercise, hypoglycaemia, hypoxia and heat exposure, and these studies are now well acknowledged. In fact, since adrenaline and noradrenaline are the main hormones whose concentrations increase markedly during exercise, many researchers have worked on the effect of exercise on these amines and reported 1.5 to >20 times basal concentrations depending on exercise characteristics (e.g. duration and intensity). Similarly, several studies have shown that adrenaline and noradrenaline are involved in cardiovascular and respiratory adjustments and in substrate mobilization and utilization. Thus, many studies have focused on physical training and gender effects on catecholamine response to exercise in an effort to verify if significant differences in catecholamine responses to exercise could be partly responsible for the different performances observed between trained and untrained subjects and/or men and women. In fact, previous studies conducted in men have used different types of exercise to compare trained and untrained subjects in response to exercise at the same absolute or relative intensity. Their results were conflicting for a while.As research progressed, parameters such as age, nutritional and emotional state have been found to influence catecholamine concentrations. As a result, most of the recent studies have taken into account all these parameters. Those studies also used very well trained subjects and/or more intense exercise, which is known to have a greater effect on catecholamine response so that differences between trained and untrained subjects are more likely to appear. Most findings then reported a higher adrenaline response to exercise in endurance-trained compared with untrained subjects in response to intense exercise at the same relative intensity as all-out exercise. This phenomenon is referred to as the ‘sports adrenal medulla’. This higher capacity to secrete adrenaline was observed both in response to physical exercise and to other stimuli such as hypoglycaemia and hypoxia. For some authors, this phenomenon can partly explain the higher physical performance observed in trained compared with untrained subjects. More recently, these findings have also been reported in anaerobic-trained subjects in response to supramaximal exercise. In women, studies remain scarce; the results are more conflicting than in men and the physical training type (aerobic or anaerobic) effects on catecholamine response remain to be specified. Conversely, the works undertaken in animals are more unanimous and suggest that physical training can increase the capacity to secrete adrenaline via an increase of the adrenal gland volume and adrenaline content.

Metabolic and Hormonal Responses to Exercise in Children and Adolescents

AbstractEthical and methodological factors limit the availability of data on metabolic and hormonal responses to exercise in children and adolescents. Despite this, it has been reported that young individuals show age-dependent responses to short and long term exercise when compared with adults.Adenosine triphosphate (ATP) and phosphocreatine stores are not age-dependent in children and adolescents. However, phosphorus-31 nuclear magnetic resonance spectroscopy (31PNMR) studies showed smaller reductions in intramuscular pH in children and adolescents during high intensity exercise than adults. Muscle glycogen levels at rest are less important in children, but during adolescence these reach levels observed in adults.Immaturity of anaerobic metabolism in children is a major consideration, and there are several possible reasons for this reduced glycolytic activity. There appear to be higher proportions of slow twitch (type I) fibres in the vastus lateralis part of the quadriceps in children than in untrained adults, and anaerobic glycolytic ATP rephosphorylation may be reduced in young individuals during high intensity exercise. Reduced activity of phosphofructokinase-1 and lactate dehydrogenase enzymes in prepubertal children could also explain the lower glycolytic capacity and the limited production of muscle lactate relative to adults. These observations may be related to reduced sympathetic responses to exhaustive resistance exercise in young people.In contrast, children and adolescents are well adapted to prolonged exercise of moderate intensity. Growth and maturation induce increases in muscle mass, with proliferation of mitochondria and contractile proteins. However, substrate utilisation during exercise differs between children and adults, with metabolic and hormonal adaptations being suggested. Lower respiratory exchange ratio values are often observed in young individuals during prolonged moderate exercise. Data indicate that children rely more on fat oxidation than do adults, and increased free fatty acid mobilisation, glycerol release and growth hormone increases in preadolescent children support this hypothesis.Plasma glucose responses during prolonged exercise are generally comparable in children and adults. When glucose is ingested at the beginning of moderate exercise, plasma glucose levels are higher in children than in adults, but this may be caused by decreased insulin sensitivity during the peripubertal period (as shown by glucose: insulin ratios). Conclusions: Children are better adapted to aerobic exercise because their energy expenditure appears to rely more on oxidative metabolism than is the case in adults. Glycolytic activity is age-dependent, and the relative proportion of fat utilisation during prolonged exercise appears higher in children than in adults.

Lactate and catecholamine responses in male and female sprinters during a Wingate test.

A total of six male and six female sprinters at the same national competition level and aged 18–20 years performed a force/velocity test and a 30-s supramaximal exercise test (Wingate test) on 2 different days, separated by a maximal interval of 15 days. The maximal anaerobic power (Wmax) was determined from the force/velocity test, and the mean anaerobic power (W) from the Wingate test. Immediately after the Wingate test, a 5-ml venous blood sample was drawn via a heparinized catheter in an antebrachial vein for subsequent catecholamine (adrenaline and noradrenaline) analysis. After 5 min recovery a few microlitres of capillary blood were also taken for an immediate lactate determination. Even expressed per kilogram lean body mass,Wmax andW were significantly lower in women. The lactate and adrenaline responses induced by the Wingate test were also less pronounced in this group whereas the noradrenaline levels were not significantly different in men and women. Above all, very different relationships appeared between lactate, adrenaline, noradenaline and W according to sex. Thus, as reported by other authors, the adrenergic response to a supramaximal exercise seemed to be lower in women than in men. Nevertheless a different training status between the two groups, even at same national competition level, could not be excluded and might contribute, at least in part, to the gender differences observed in the present study.

Inverse relationship between percentage body weight change and finishing time in 643 forty-two-kilometre marathon runners

Objective The purpose of this study was to determine the relationship between athletic performance and the change in body weight (BW) during a 42 km marathon in a large cohort of runners. Methods The study took place during the 2009 Mont Saint-Michel Marathon (France). 643 marathon finishers (560 males and 83 females) were studied. The change in BW during the race was calculated from measurements of each runners BW immediately before and after the race. Results BW loss was 2.3±2.2% (mean±SEM) (p<0.01). BW loss was −3.1±1.9% for runners finishing the marathon in less than 3 h; −2.5±2.1% for runners finishing between 3 and 4 h; and −1.8±2.4% for runners who required more than 4 h to complete the marathon. The degree of BW loss was linearly related to 42 km race finishing time (p<0.0000001). Neither age nor gender influenced BW loss during the race. Conclusions BW loss during the marathon was inversely related to race finishing time in 643 marathon runners and was >3% in runners completing the race in less than 3 h. These data are not compatible with laboratory-derived data suggesting that BW loss greater than 2% during exercise impairs athletic performance. They match an extensive body of evidence showing that the most successful athletes in marathon and ultra-marathon running and triathlon events are frequently those who lose substantially more than 3–4% BW during competition.

Glucose and free fatty acid utilization during prolonged exercise in prepubertal boys in relation to catecholamine responses.

SummaryTen prepubertal boys performed 60-min cycle exercise at about 60% of their maximal oxygen uptake as previously measured. To measure packed cell volume, plasma glucose, free fatty acids (FFA), glycerol and catecholamines, blood samples were drawn at rest using a heparinized cathether and at the 15th, 30th and 60th min of the exercise and after 30 min of recovery. At rest, the blood glucose concentrations were at the lowest values for normal. Exercise induced a small decrease of blood glucose which was combined with an abrupt increase of the noradrenaline concentration during the first 15 min. The FFA and glycerol concentrations increased throughout the exercise linearly with that of adrenaline. Compared to adults, the FFA uptake expressed per minute and per litre of oxygen uptake was greater in children. These results suggested that it is difficult for children to maintain a constant blood glucose concentration and that prolonged exercise provided a real stimulus to hypoglycaemia. An immediate and large increase in noradrenaline concentration during exercise and a greater utilization of FFA was probably used by children to prevent hypoglycaemia.

Activity, inactivity and quality of life among Lebanese adolescents

Background:  The aim of the present study was to investigate recent overweight and obesity prevalence rates for Lebanese adolescents, and to examine differences in physical activity, screen time (sum of time spent in front of TV, computer, and videogames), and health‐related quality of life (HRQOL) for the first time among normal, overweight, and obese adolescents.

Intense exercise training induces adaptation in expression and responsiveness of cardiac β-adrenoceptors in diabetic rats

BackgroundInformations about the effects of intense exercise training on diabetes-induced myocardial dysfunctions are lacking. We have examined the effects of intense exercise training on the cardiac function of diabetic rats, especially focusing on the Langendorff β-adrenergic responsiveness and on the β-adrenoceptors protein expression.MethodsControl or Streptozotocin induced-diabetic male Wistar rats were randomly assigned to sedentary or trained groups. The training program consisted of 8 weeks running on a treadmill (10° incline, up to 25 m/min, 60 min/day) and was considered to be intense for diabetic rats.ResultsThis intense exercise training amplified the in vivo diabetes-induced bradycardia. It had no effect on Langendorff basal cardiac contraction and relaxation performances in control and diabetic rats. In diabetic rats, it accentuated the Langendorff reduced responsiveness to β-adrenergic stimulation. It did not blunt the diabetes-induced decrease of β1-adrenoceptors protein expression, displayed a significant decrease in the β2-adrenoceptors protein expression and normalized the β3-adrenoceptors protein expression.ConclusionsIntense exercise training accentuated the decrease in the myocardial responsiveness to β-adrenergic stimulation induced by diabetes. This defect stems principally from the β2-adrenoceptors protein expression reduction. Thus, these results demonstrate that intense exercise training induces specific effects on the β-adrenergic system in diabetes.

Normal physical working capacity in prepubertal children with type 1 diabetes compared with healthy controls.

Background: Exercise testing has become a valuable help for the physician to examine the influence of recommended exercise training on physical fitness. However, the question as to how diabetic prepubertal children differ from their non‐diabetic peers in their performance capacity has only partial and sometimes conflicting answers in the literature. Aim and methods: The aim of the current study was thus to evaluate aerobic fitness during an incremental submaximal test (measure of the Physical Working Capacity 170 (PWC170)) in 17 well‐controlled prepubertal insulin‐dependent diabetic boys aged 8.5–13 y. Eighteen healthy prepubertal boys matched for age, body size and physical activity served as controls. Part of the method was to check capillary blood glucose level in the diabetic patients and in nine of the healthy subjects throughout the exercise. Results: From this experiment it appeared that the level of physical fitness was similar in diabetic and healthy boys (PWC170 2.28±0.09 vs 2.37±0.13 W·kg−1). While glucose homeostasis was well maintained in the healthy group, diabetic children showed a marked fall in blood glucose during the exercise. In addition, the PWC170 level correlated significantly with the estimate of energy expenditure attributed to vigorous activities in the diabetic boys.

Glucoregulation and hormonal changes during prolonged exercise in boys and girls

A group of 17 children, 8.5–11 years old, performed a 60-min cycle exercise at 60% of maximal oxygen uptake (VO2max) 2 h after a standardized breakfast. They were 10 young boys (pubertal stage =1) and 7 young girls (pubertal stage ⩽2) of similarVO2max (respective values were 48.5 ml min−1 kg−1, SEM 1.8; 42.1 ml min−1 kg−1, SEM 2.4). Blood samples of 5 ml were withdrawn by heparinized catheter, the subjects being in a supine position, 30 min before the test, then after 0, 15, 30 and 60 min of exercise and following 30 min recovery. Haematocrit was immediately measured. Thereafter plasma was analysed for glucose, non-esterified fatty acid, glycerol, catecholamine (noradrenaline, adrenaline), insulin and glucagon concentrations. This study showed two main results. First, the onset of exercise induced a significant glucose decrease (of about 11,4%) in all the children. Secondly, both the glycaemic and the hormonal responses were obviously different according to the sex. In boys only, the initial glucose drop was significantly correlated to the pre-exercise insulin values. Whatever the time, the glycaemic levels and the catecholamine responses were lower in girls than in boys, whereas the insulin values remained higher. However, none of these two hormonal parameters seemed to be really responsible for the lower glucose values in girls. On the one hand, the great individual variability of noradrenaline and adrenaline and differences in their relative intensity at the end of the exercise between boys and girls might contribute to the lower catecholamine levels in girls. On the other hand, the lack of a significant relationship in girls between the glucose decrease after exercise and the pre-exercise insulin values might be explained by a relative insulin insensitivity concomitant with the earlier growth spurt in girls, as demonstrated in subjects at rest by other authors. Finally the mechanisms of all these gender differences remain to be clarified and might be accounted for by a different maturation level in boys and girls.

Professional tennis players' serve: correlation between segmental angular momentums and ball velocity

The purpose of the study was to identify the relationships between segmental angular momentum and ball velocity between the following events: ball toss, maximal elbow flexion (MEF), racket lowest point (RLP), maximal shoulder external rotation (MER), and ball impact (BI). Ten tennis players performed serves recorded with a real-time motion capture. Mean angular momentums of the trunk, upper arm, forearm, and the hand-racket were calculated. The anteroposterior axis angular momentum of the trunk was significantly related with ball velocity during the MEF–RLP, RLP–MER, and MER–BI phases. The strongest relationships between the transverse-axis angular momentums and ball velocity followed a proximal-to-distal timing sequence that allows the transfer of angular momentum from the trunk (MEF–RLP and RLP–MER phases) to the upper arm (RLP–MER phase), forearm (RLP–MER and MER–BI phases), and the hand-racket (MER–BI phase). Since sequence is crucial for ball velocity, players should increase angular momentums of the trunk during MEF–MER, upper arm during RLP–MER, forearm during RLP–BI, and the hand-racket during MER–BI.