Paulo Branco

Prioritizing barrier removal to improve functional connectivity of rivers

Summary 1. Freshwater systems are severely impacted by connectivity reduction due to the construction of dams and weirs. The breach of this longitudinal connectivity imperils freshwater fish species world-wide. There is thus an increasing need for numerical tools that help decisionmakers correctly allocate resources to prioritize restoration actions. 2. This study provides a methodology for prioritizing the removal of barriers. It is based on spatial graphs, which represent structural units as nodes and relationships between nodes as links, and uses habitat suitability modelling (Boosted Regression Trees) to weight nodes. To exemplify the application of this procedure, we used the Tagus River network and evaluated the impact of the dams (29 built between 1928 and 2004) on the occurrence of each of two fish species (Iberian barbel Luciobarbus bocagei – representing large potamodromous fish; and southern Iberian chub Squalius pyrenaicus – representing small water-column residents) and on the combination of both. 3. Results show that dam construction on the Tagus was responsible for a 484–544% reduction in river connectivity for different fish species. Actions to promote connectivity in just seven of the dams would increase connectivity by 350–372%. 4. The removal of a single barrier chosen through prioritization had a greater overall connectivity increase than the random removal of seven barriers. 5. Synthesis and applications. The proposed prioritization method, using spatial graphs and habitat suitability modelling, makes it possible to model the impact of the removal or placement of an insurmountable barrier on the overall functional connectivity of a river network, facilitating resource allocation and minimizing the impact of new barrier implementation.

Pool-type fishways: two different morpho-ecological cyprinid species facing plunging and streaming flows.

Fish are particularly sensitive to connectivity loss as their ability to reach spawning grounds is seriously affected. The most common way to circumvent a barrier to longitudinal connectivity, and to mitigate its impacts, is to implement a fish passage device. However, these structures are often non-effective for species with different morphological and ecological characteristics so there is a need to determine optimum dimensioning values and hydraulic parameters. The aim of this work is to study the behaviour and performance of two species with different ecological characteristics (Iberian barbel Luciobarbus bocagei–bottom oriented, and Iberian chub Squalius pyrenaicus–water column) in a full-scale experimental pool-type fishway that offers two different flow regimes–plunging and streaming. Results showed that both species passed through the surface notch more readily during streaming flow than during plunging flow. The surface oriented species used the surface notch more readily in streaming flow, and both species were more successful in moving upstream in streaming flow than in plunging flow. Streaming flow enhances upstream movement of both species, and seems the most suitable for fishways in river systems where a wide range of fish morpho-ecological traits are found.

Electric bicycle using batteries and supercapacitors

In this paper, a traction system useful for an autonomous Electric Vehicle of individual use is described. The developed system is constituted in a first approach by two different power sources: one is constituted by batteries or by fuel cells, and the other by supercapacitors. This paper describes a technical solution joining and accomplishing the usage of two energy storage systems in the same traction system. In the developed system, the supercapacitors run as element that store energy temporarily and that can be used to retrieve energy. Starting from the functional characteristics of typical electrical vehicles and characterization of a typical routing profile, the energy consumption is obtained. In order to characterize and design the system, this is described in detail, namely the supercapacitors models, the battery, the power converters and the implemented strategy of control. According to the obtained results, a control strategy that allows an effective management of the stored energy in the system regarding the vehicles optimal functioning and increasing its autonomy is also presented and discussed. Based on experimental and simulation results, the advantages and disadvantages of the proposed solution are presented.

New trends in recognizing experimental drives: fuzzy logic and formal language theories

Drive systems today determine the productivity and quality of industrial processes. However, they exhibit considerable complexities related with their behavior as large uncertainties at a structure and parameter levels, multidimensionality, and strong mutual interactions. This paper aims to analyze common features, and the potential, but also the drawbacks that fuzzy logic and formal language theories show when used for recognition of patterns in experimental drives. Two prototype systems are used: an electrohydraulic drive and an induction motor drive. We underline the similarities and various aspects of the recognition methodologies, despite their use on different systems. A set of experimental learning situations with critical effects on their performance are presented and discussed.

Potamodromous fish movements under multiple stressors: Connectivity reduction and oxygen depletion.

Rivers are impacted by multiple stressors that can interact to create synergistic, additive or antagonistic effects, but experimental studies on fish encompassing more than one stressor are seldom found. Thus, there is the need to study stressors through multifactorial approaches that analyse the impact of fish exposure to multiple stressors and evaluate fish sensitivity to stressor combinations. Some of the most common impacts to Mediterranean rivers are of two natures: i) water abstraction and ii) diffuse pollution. Therefore, the present study aims at studying the responses of potamodromous fish facing combinations of: 1) a primary stressor (two levels of connectivity reduction due to water scarcity), and 2) a secondary stressor (three levels of oxygen depletion due to increase organic load - of anthropogenic nature). Schools of five wild fish from a cyprinid species (Luciobarbus bocagei) were placed in a flume, equipped with see-through sidewalls to allow for behavioural analysis, and subjected to different combinations of the stressors. Results show that at the unconnected level the primary stressor (lack of connectivity) overrode the effect of the secondary stressor (oxygen depletion), but when connectivity existed oxygen depletion caused a reduction of fish movements with decreasing oxygen concentrations. This multifactorial study contributes to improved prediction of fish responses upon actual or projected pressure scenarios.

Sensitivity of river fishes to climate change: The role of hydrological stressors on habitat range shifts.

Climate change will predictably change hydrological patterns and processes at the catchment scale, with impacts on habitat conditions for fish. The main goal of this study is to assess how shifts in fish habitat favourability under climate change scenarios are affected by hydrological stressors. The interplay between climate and hydrological stressors has important implications in river management under climate change because management actions to control hydrological parameters are more feasible than controlling climate. This study was carried out in the Tamega catchment of the Douro basin. A set of hydrological stressor variables were generated through a process-based modelling based on current climate data (2008-2014) and also considering a high-end future climate change scenario. The resulting parameters, along with climatic and site-descriptor variables were used as explanatory variables in empirical habitat models for nine fish species using boosted regression trees. Models were calibrated for the whole Douro basin using 254 fish sampling sites and predictions under future climate change scenarios were made for the Tamega catchment. Results show that models using climatic variables but not hydrological stressors produce more stringent predictions of future favourability, predicting more distribution contractions or stronger range shifts. The use of hydrological stressors strongly influences projections of habitat favourability shifts; the integration of these stressors in the models thinned shifts in range due to climate change. Hydrological stressors were retained in the models for most species and had a high importance, demonstrating that it is important to integrate hydrology in studies of impacts of climate change on freshwater fishes. This is a relevant result because it means that management actions to control hydrological parameters in rivers will have an impact on the effects of climate change and may potentially be helpful to mitigate its negative effects on fish populations and assemblages.

Do small barriers affect the movement of freshwater fish by increasing residency

River fragmentation due to artificial barriers directly impacts fish communities by limiting migratory movements. This work aims to understand how small barriers affect the movements of a potamodromous cyprinid species - the Iberian barbel (Luciobarbus bocagei (Steindachner, 1864)) - in a 5.6km upstream segment of a stream impacted only by the presence of physical barriers. Fish were marked with Visible Implant Elastomer tags (VIE), and barriers were seasonally characterized. A total of 683 fish were tagged, with 104 recaptures, during five sampling seasons (spring 2012 - early summer 2013). Eleven of the recaptured fish moved past a barrier, without any preference in terms of direction of movement. There were no differences in length, either between migrants and residents, or between upstream and downstream migrants. The results show that although barbel are able to negotiate small barriers, part of the population did not move between fragmented reaches - an indication that under such conditions, fish species populations may adjust their life-history strategy to augment residency as it was hypothesized from the results.

Broad-scale sampling of primary freshwater fish populations reveals the role of intrinsic traits, inter-basin connectivity, drainage area and latitude on shaping contemporary patterns of genetic diversity

Background. Worldwide predictions suggest that up to 75% of the freshwater fish species occurring in rivers with reduced discharge could be extinct by 2070 due to the combined effect of climate change and water abstraction. The Mediterranean region is considered to be a hotspot of freshwater fish diversity but also one of the regions where the effects of climate change will be more severe. Iberian cyprinids are currently highly endangered, with over 68% of the species raising some level of conservation concern. Methods. During the FISHATLAS project, the Portuguese hydrographical network was extensively covered (all the 34 river basins and 47 sub-basins) in order to contribute with valuable data on the genetic diversity distribution patterns of native cyprinid species. A total of 188 populations belonging to 16 cyprinid species of Squalius, Luciobarbus, Achondrostoma, Iberochondrostoma, Anaecypris and Pseudochondrostoma were characterized, for a total of 3,678 cytochrome b gene sequences. Results. When the genetic diversity of these populations was mapped, it highlighted differences among populations from the same species and between species with identical distribution areas. Factors shaping the contemporary patterns of genetic diversity were explored and the results revealed the role of latitude, inter-basin connectivity, migratory behaviour, species maximum size, species range and other species intrinsic traits in determining the genetic diversity of sampled populations. Contrastingly, drainage area and hydrological regime (permanent vs. temporary) seem to have no significant effect on genetic diversity. Species intrinsic traits, maximum size attained, inter-basin connectivity and latitude explained over 30% of the haplotype diversity variance and, generally, the levels of diversity were significantly higher for smaller sized species, from connected and southerly river basins. Discussion. Targeting multiple co-distributed species of primary freshwater fish allowed us to assess the relative role of historical versus contemporary factors affecting genetic diversity. Since different patterns were detected for species with identical distribution areas we postulate that contemporary determinants of genetic diversity (species’ intrinsic traits and landscape features) must have played a more significant role than historical factors. Implications for conservation in a context of climate change and highly disturbed habitats are detailed, namely the need to focus management and conservation actions on intraspecific genetic data and to frequently conduct combined genetic and demographic surveys.

Historical changes in the functional connectivity of rivers based on spatial network analysis and the past occurrences of diadromous species in Portugal

The disruption of longitudinal connectivity caused by artificial barriers in rivers is considered to be a major threat to freshwater fish communities at the global scale. In Portugal, the construction of several big dams all through the 20th century was a major cause of disappearance or decline of long distance migratory fish species from various rivers. In this study we analysed the historical changes of functional connectivity for diadromous fish at the Tagus basin river network (Portugal), using a spatial graph approach that integrates both structural connectivity and habitat suitability. Due to the presence of many artificial barriers, the current distribution of species does not reflect natural environmental conditions. Therefore, historical data on the occurrence of species is paramount to assess the potential habitat suitability. The compilation of historical data on the distribution of diadromous species [Sea Lamprey—Petromyzon marinus (Linnaeus 1758) and Allis shad—Alosa alosa (Linnaeus 1758)] was based on geographical dictionaries and geo-referenced information for Portugal dated between 1700 and 1900. Habitat suitability was expressed as the probabilities of occurrence of empirical predictive models based on the historical records and a set of regionalized environmental data. The loss of connectivity caused by big dam construction between 1928 and 2004 was then analysed using a spatial graph approach. The output of this analysis will aid decision-makers to optimize targets of connectivity restoration actions aiming at enhancing the functional connectivity of the river network for diadromous species, using the historical situation as a benchmark.

Macroinvertebrate short-term responses to flow variation and oxygen depletion: A mesocosm approach

In Mediterranean rivers, water scarcity is a key stressor with direct and indirect effects on other stressors, such as water quality decline and inherent oxygen depletion associated with pollutants inputs. Yet, predicting the responses of macroinvertebrates to these stressors combination is quite challenging due to the reduced available information, especially if biotic and abiotic seasonal variations are taken under consideration. This study focused on the response of macroinvertebrates by drift to single and combined effects of water scarcity and dissolved oxygen (DO) depletion over two seasons (winter and spring). A factorial design of two flow velocity levels - regular and low (vL) - with three levels of oxygen depletion - normoxia, medium depletion (dM) and higher depletion (dH) - was carried out in a 5-artificial channels system, in short-term experiments. Results showed that both stressors individually and together had a significant effect on macroinvertebrate drift ratio for both seasons. Single stressor effects showed that macroinvertebrate drift decreased with flow velocity reduction and increased with DO depletion, in both winter and spring experiments. Despite single stressors opposing effects in drift ratio, combined stressors interaction (vL×dM and vL×dH) induced a positive synergistic drift effect for both seasons, but only in winter the drift ratio was different between the levels of DO depletion. Stressors interaction in winter seemed to intensify drift response when reached lower oxygen saturation. Also, drift patterns were different between seasons for all treatments, which may depend on individuals life stage and seasonal behaviour. Water scarcity seems to exacerbate the oxygen depletion conditions resulting into a greater drifting of invertebrates. The potential effects of oxygen depletion should be evaluated when addressing the impacts of water scarcity on river ecosystems, since flow reductions will likely contribute to a higher oxygen deficit, particularly in Mediterranean rivers.