Paulo Eduardo Bennemann

Desempenho reprodutivo de fêmeas suínas submetidas à inseminação artificial intra-uterina ou à tradicional

A total of 298 Camborough 22® sows was distributed in two treatments: T1 (n=154): intrauterine insemination (IUI) with 0.5 billion sperms in 20ml total volume; T2 (n=144): traditional insemination (TAI) with 3.0 billion sperms in 90ml total volume. It was possible to perform the IUI in 98.1% of sows. It was observed presence of blood on the catheter tip or pipete in 8.4% of IUI sows. The pregnancy (PR) and adjusted farrowing rates did not differ (P>0.05) among treatments. Litter size (LS) differ among treatments (P<0.05), being observed a reduction of 0.8 piglet in IUI. The blood presence in the IUI did not affect the PR significantly, but resulted in a reduction of 2.6 piglets per litter.

A hipertermia durante o estro pode afetar o desempenho reprodutivo de fêmeas suínas

The objective of this study was to evaluate the effect of hyperthermia at the day of artificial insemination (AI) up to day 13th on reproductive performance of gilts and sows. In the experiment 1, 488 females were divided in two groups according to the rectal temperature: G1= £ 39.5oC (normothermia), and G2 = > 39.5oC (hiperthermia). The females having hyperthermia showed higher return to estrus rate (ERR), and lower farrowing rate (FR) and litter size (LS) (p<0.05) than females having normothermia. In the second experiment, 764 females were grouped in four groups: G1 = hyperthermia on the day of AI; G2 = hyperthermia on one of the first four days after AI; G3 = hyperthermia in one day of the first two groups, and, G4 = hyperthermia betwen 10-13th day of pregnancy. Females having hyperthermia at the day of the first AI and during the first four days after AI, had a higher ERR and lower FR (p<0.01) in G1 and G2, and lower LS in G1, G2 and G3 (p<0.01), than the females with normothermia. There were not differences among the females havig hyperthermia or normothermia in G4. In the experiment 3, from 102 gilts, 88 were slaughtered from 29 to 34 days after AI. The ERR were higher (p<0.01)and the pregnant rate (PR) at up to 24 days, the number of viable embryos (NVE) and the embryo survival rate (ESR) were lower in females having hyperthermia (p<0.05). In conclusion, hyperthermia at the day of the first AI and during the first four days after AI can be deleterious to the female reproductive performance.

MOTILIDADE ESPERMÁTICA E INTEGRIDADE ACROSSOMAL EM DOSES DE SÊMEN SUÍNO REFRIGERADAS E INOCULADAS COM ESCHERICHIA COLI E STAPHYLOCOCCUS AUREUS

Twelve ejaculates were collected in the most aseptic manner and distributed in seven treatments (control group T1). Semen were inoculated with S. aureus (T2, T3 e T4) or E. coli (T5, T6 e T7) in three concentrations (5 x 105, 5 x 106 and 5 x 107 CFU/ml). The sperm motility, the percentage of normal apical range (NAR), the pH and the number of colony unit former (CFU/ml) of bacteria, for 96 hours, were evaluated. The bacterial development was decreasing during the first 96 hours. Except for the treatment with the inoculation of 5 x 107 CFU of E. coli/ml, there was no significant effect of the bacteria on the sperm motility (p>0.05). Also, there was no significant effect (p>0.05) of the S. aureus or E. coli on the percent of NAR and the pH during the 96 hours. When compared to the control group, only the inoculation of 5 x 107 CFU/ml of E. coli differed at the 96 hours in relation to the percentage of NAR (p<0.05). There was no correlation among the sperm motility, NAR, pH and CFU.Foram utilizados 12 ejaculados, coletados de maneira asseptica, distribuidos em sete tratamentos, sendo um grupo controle e os demais inoculados com tres diferentes concentracoes de S. aureus ou E. coli (5 x 105, 5 x 106 e 5 x 107 UFC/ml). Durante 96 horas, foram avaliados a motilidade espermatica, o percentual de acrossomas intactos (NAR), o pH e o numero de unidades formadoras de colonia (UFC/ml) das bacterias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com excecao do tratamento com a inoculacao de 5 x 107 UFC de E. coli/ml, nao foi observado efeito significativo das bacterias sobre a motilidade espermatica (p>0,05). Da mesma forma, nao houve efeito significativo (p>0,05) do S. aureus ou da E. coli sobre o percentual de NAR e pH nas 96 horas. Quando comparado ao controle, somente a inoculacao de 5 x 107 UFC/ml de E. coli diferiu, as 96 horas, em relacao ao percentual de NAR (p£0,05). Nao foi observada correlacao entre as variaveis motilidade, NAR, pH e UFC.

Lesões em linfonodos de suínos causadas por Mycobacterium grupo III (Runyon)

Condenacoes de suinos no momento do abate devido a lesoes tuberculoides causadas por micobacterias sao descritas em diversos paises, inclusive no Brasil. No presente trabalho, 146 animais de idades variando entre 2,5 e > 14 meses, provenientes de uma propriedade com problema de condenacoes por tuberculose foram abatidos e os linfonodos examinados quanto ao tipo de lesao macro e microscopica e a presenca de micobacterias. Ao mesmo tempo, a racao, a agua e a maravalha utilizadas na propriedade foram investigadas quanto a presenca de micobacterias. Macroscopicamente, os linfonodos afetados apresentavam nodulos arredondados de 1-2 mm de diâmetro, de coloracao amarelo-clara com conteudo pastoso a caseoso, delimitados por uma capsula. Em linfonodos de 12 animais foi possivel visualizar bacilos alcool-acido resistentes, sendo que em 4 deles foi isolado o Mycobacterium-grupo III (Runyon). As lesoes foram encontradas principalmente em animais entre 6 e 14 meses de idade. A mesma bacteria foi isolada da maravalha, que pode ter sido a fonte de infeccao dos animais.

SPERM MOTILITY AND ACROSSOMAL INTEGRITY IN LIQUID BOAR SEMEN DOSIS INOCULATED WITH ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS

Twelve ejaculates were collected in the most aseptic manner and distributed in seven treatments (control group T1). Semen were inoculated with S. aureus (T2, T3 e T4) or E. coli (T5, T6 e T7) in three concentrations (5 x 105, 5 x 106 and 5 x 107 CFU/ml). The sperm motility, the percentage of normal apical range (NAR), the pH and the number of colony unit former (CFU/ml) of bacteria, for 96 hours, were evaluated. The bacterial development was decreasing during the first 96 hours. Except for the treatment with the inoculation of 5 x 107 CFU of E. coli/ml, there was no significant effect of the bacteria on the sperm motility (p>0.05). Also, there was no significant effect (p>0.05) of the S. aureus or E. coli on the percent of NAR and the pH during the 96 hours. When compared to the control group, only the inoculation of 5 x 107 CFU/ml of E. coli differed at the 96 hours in relation to the percentage of NAR (p<0.05). There was no correlation among the sperm motility, NAR, pH and CFU.Foram utilizados 12 ejaculados, coletados de maneira asseptica, distribuidos em sete tratamentos, sendo um grupo controle e os demais inoculados com tres diferentes concentracoes de S. aureus ou E. coli (5 x 105, 5 x 106 e 5 x 107 UFC/ml). Durante 96 horas, foram avaliados a motilidade espermatica, o percentual de acrossomas intactos (NAR), o pH e o numero de unidades formadoras de colonia (UFC/ml) das bacterias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com excecao do tratamento com a inoculacao de 5 x 107 UFC de E. coli/ml, nao foi observado efeito significativo das bacterias sobre a motilidade espermatica (p>0,05). Da mesma forma, nao houve efeito significativo (p>0,05) do S. aureus ou da E. coli sobre o percentual de NAR e pH nas 96 horas. Quando comparado ao controle, somente a inoculacao de 5 x 107 UFC/ml de E. coli diferiu, as 96 horas, em relacao ao percentual de NAR (p£0,05). Nao foi observada correlacao entre as variaveis motilidade, NAR, pH e UFC.

Sperm motility and acrosomal integrity in boar semen inoculated with Escherichia coli and Staphylococcus aureus.

Twelve ejaculates were collected in the most aseptic manner and distributed in seven treatments (control group T1). Semen were inoculated with S. aureus (T2, T3 e T4) or E. coli (T5, T6 e T7) in three concentrations (5 x 105, 5 x 106 and 5 x 107 CFU/ml). The sperm motility, the percentage of normal apical range (NAR), the pH and the number of colony unit former (CFU/ml) of bacteria, for 96 hours, were evaluated. The bacterial development was decreasing during the first 96 hours. Except for the treatment with the inoculation of 5 x 107 CFU of E. coli/ml, there was no significant effect of the bacteria on the sperm motility (p>0.05). Also, there was no significant effect (p>0.05) of the S. aureus or E. coli on the percent of NAR and the pH during the 96 hours. When compared to the control group, only the inoculation of 5 x 107 CFU/ml of E. coli differed at the 96 hours in relation to the percentage of NAR (p<0.05). There was no correlation among the sperm motility, NAR, pH and CFU.Foram utilizados 12 ejaculados, coletados de maneira asseptica, distribuidos em sete tratamentos, sendo um grupo controle e os demais inoculados com tres diferentes concentracoes de S. aureus ou E. coli (5 x 105, 5 x 106 e 5 x 107 UFC/ml). Durante 96 horas, foram avaliados a motilidade espermatica, o percentual de acrossomas intactos (NAR), o pH e o numero de unidades formadoras de colonia (UFC/ml) das bacterias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com excecao do tratamento com a inoculacao de 5 x 107 UFC de E. coli/ml, nao foi observado efeito significativo das bacterias sobre a motilidade espermatica (p>0,05). Da mesma forma, nao houve efeito significativo (p>0,05) do S. aureus ou da E. coli sobre o percentual de NAR e pH nas 96 horas. Quando comparado ao controle, somente a inoculacao de 5 x 107 UFC/ml de E. coli diferiu, as 96 horas, em relacao ao percentual de NAR (p£0,05). Nao foi observada correlacao entre as variaveis motilidade, NAR, pH e UFC.