Pei-Chia Chan
National Yang-Ming University
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Publication
Featured researches published by Pei-Chia Chan.
Biochimica et Biophysica Acta | 2015
Cheng-Han Tsai; Liang-Ting Lin; Chung-Yih Wang; Yu-Wen Chiu; Yen-Ting Chou; Shu-Jun Chiu; Hsin-Ell Wang; Ren-Shyan Liu; Chun-Yi Wu; Pei-Chia Chan; Muh-Hwa Yang; Shih-Hwa Chiou; Man-Jyun Liao; Yi-Jang Lee
Cofilin-1, a non-muscle isoform of actin regulatory protein that belongs to the actin-depolymerizing factor (ADF)/cofilin family is known to affect cancer development. Previously, we found that over-expression of cofilin-1 suppressed the growth and invasion of human non-small cell lung cancer (NSCLC) cells in vitro. In this study, we further investigated whether over-expression of cofilin-1 can suppress tumor growth in vivo, and performed a microRNA array analysis to better understand whether specific microRNA would be involved in this event. The results showed that over-expression of cofilin-1 suppressed NSCLC tumor growth using the xenograft tumor model with the non-invasive reporter gene imaging modalities. Additionally, cell motility and invasion were significantly suppressed by over-expressed cofilin-1, and down-regulation of matrix metalloproteinase (MMPs) -1 and -3 was concomitantly detected. According to the microRNA array analysis, the let-7 family, particularly let-7b and let-7e, were apparently up-regulated among 248 microRNAs that were affected after over-expression of cofilin-1 up to 7 days. Knockdown of let-7b or let-7e using chemical locked nucleic acid (LNA) could recover the growth rate and the invasion of cofilin-1 over-expressing cells. Next, the expression of c-myc, LIN28 and Twist-1 proteins known to regulate let-7 were analyzed in cofilin-1 over-expressing cells, and Twist-1 was significantly suppressed under this condition. Up-regulation of let-7 microRNA by over-expressed cofilin-1 could be eliminated by co-transfected Twist-1 cDNA. Taken together, current data suggest that let-7 microRNA would be involved in over-expression of cofilin-1 mediated tumor suppression in vitro and in vivo.
Molecular Imaging and Biology | 2014
Chun-Yi Wu; Pei-Chia Chan; Lin-Shan Chou; Chi-Wei Chang; Feng-Yi Yang; Ren-Shyan Liu; Shih-Hwa Chiou; Yi-Wei Chen; Sang-Hue Yen; Hsin-Ell Wang
PurposeThis study aims to demonstrate that pulsed high-intensity focused ultrasound (pulsed-HIFU) may enhance the fructose-conjugated 4-borono-L-phenylalanine (BPA-Fr) accumulation in tumor lesion using 18F-FBPA-Fr microPET scans.ProceduresTo the mice bearing orthotopic SASC03 human tongue squamous carcinoma xenograft, a 2-min pulsed-HIFU was applied to tumor. Immediately after pulsed-HIFU treatment, 18F-FBPA-Fr was intravenously injected, and biological characterizations including microPET imaging and biodistribution were conducted.ResultsBoth biodistribution studies and microPET imaging performed after intravenous injection of 18F-FBPA-Fr revealed higher tumor uptake in HIFU-treated mice than that of the control. CD31 and Ki-67 histochemical staining of tumor sections and H&E staining of nearby normal tissues revealed no significant difference between the pulsed-HIFU-treated mice and the control.ConclusionThis study demonstrated that pulsed-HIFU was beneficial to the accumulation of boron drug in the head and neck tumor lesion and may enhance the therapeutic efficacy of clinical BNCT.
Molecular Imaging and Biology | 2015
Pei-Chia Chan; Chun-Yi Wu; Lin-Shan Chou; Chung-Hsien Ho; Chi-Wei Chang; Shih-Hwa Chiou; Wuu-Jyh Lin; Fu-Du Chen; C. Allen Chang; Jeng-Jong Hwang; Ren-Shyan Liu; Hsin-Ell Wang
PurposeThis study employed 3′-deoxy-3′-[18F]-fluorothymidine ([18F]FLT) microPET scanning to assess the treatment response of histone deacetylase inhibitors (HDACi), e.g., N1-hydroxy-N8-phenyloctanediamide (SAHA) and its iodinated derivative ISAHA, in a hepatoma mouse model.ProceduresThe in vitro cytotoxicity of HDACi in various hepatoma cell lines was determined by MTT assay and flow cytometry. ISAHA and SAHA were used to treat HepG2 hepatoma xenograft-bearing mice. The treatment responses were characterized in terms of tumor burden, microPET imaging, and immunohistochemical staining of tumor sections.ResultsISAHA effectively inhibited HepG2 hepatoma cell survival and tumor growth. A significantly reduced tumor uptake during HDACi treatment was noticed in [18F]FLT microPET imaging, which was consistent with the findings in immunohistochemical staining.ConclusionsISAHA can suppress tumor cell proliferation both in vitro and in vivo. [18F]FLT PET is a promising modality for evaluating the in vivo therapeutic efficacy of HDACi at the early stage of treatment.
Molecular Imaging and Biology | 2013
Chun-Yi Wu; Lin-Shan Chou; Pei-Chia Chan; Chung-Hsien Ho; Ming-Hsien Lin; Chih-Chieh Shen; Ren-Shyan Liu; Wuu-Jyh Lin; Hsin-Ell Wang
Journal of Biomedical Science | 2016
Jia-Je Li; Shun-Fu Chang; I-Iu Liau; Pei-Chia Chan; Ren-Shyan Liu; Sang-Hue Yen; Hsin-Ell Wang; Cheng Allen Chang
Archive | 2017
Cheng Allen Chang; Keng-Li Lan; Hsin-Ell Wang; Shun-Fu Chang; Jia-je Li; Pei-Chia Chan
Molecular Imaging and Biology | 2017
Chun-Yi Wu; Jo-Hsin Tang; Pei-Chia Chan; Jia-Je Li; Ming-Hsien Lin; Chih-Chieh Shen; Ren-Shyan Liu; Hsin-Ell Wang
Society of Nuclear Medicine Annual Meeting Abstracts | 2014
Jo-Hsin Tang; Kuo-Yi Kan; Chun-Yi Wu; Pei-Chia Chan; Chao-Cheng Chen; Ming-Hsien Lin; Hsin-Ell Wang
Society of Nuclear Medicine Annual Meeting Abstracts | 2013
Pei-Chia Chan; Chun-Yi Wu; Chi-Wei Chang; Feng-Yi Yang; Ren-Shyan Liu; Sang-Hue Yen; Hsin-Ell Wang
Society of Nuclear Medicine Annual Meeting Abstracts | 2013
Chao-Cheng Chen; Jia-Je Li; Pei-Chia Chan; Keng-Li Lan; Jo-Hsin Tang; Yu-Ling Hsieh; Ren-Shyan Liu; C. Allen Chang; Hsin-Ell Wang